LOCUS       sequence-481896-        2892 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Overexpress mutant alpha-Synuclein protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   McGlinchey RP, Ramos S, Dimitriadis EK, Wilson CB, Lee JC
  TITLE     Defining essential charged residues in fibril formation of a 
            lysosomal derived N-terminal alpha-synuclein truncation.
  JOURNAL   Nat Commun. 2025 Apr 23;16(1):3825. doi: 10.1038/s41467-025-58899-9.
  PUBMED    40268916
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-04-23"; volume: "16(1):3825. doi"; pages: " 
            10.1038/s41467-025-58899-9"
COMMENT     Sequence Label: pT7-7 asyn K96A
FEATURES             Location/Qualifiers
     source          1..2892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(42..833,834..902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     665..684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(903..1007)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1075..1093
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      1222..1810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1711..1730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1964..1981
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2216..2238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2303..2322
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2303..2321
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2353..2375
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
       61 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      121 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      181 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      241 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      301 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
      361 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
      421 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
      481 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
      541 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
      601 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
      661 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
      721 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
      781 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
      841 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
      901 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      961 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1021 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1081 cgtatcacga ggccctttcg tcttcaagaa taaaaggatc taggtgaaga tcctttttga
     1141 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1681 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1741 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1801 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1861 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1921 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1981 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2041 ccgcatagga agatcttccg gaagatcttc ctatggtgca ctctcagtac aatctgctct
     2101 gatgcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     2161 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2221 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggcc cagcgattcg
     2281 aacttctgat agacttcgaa attaatacga ctcactatag ggagaccaca acggtttccc
     2341 tctagaaata attttgttta actttaagaa ggagatatac atatggatgt attcatgaaa
     2401 ggactttcaa aggccaagga gggagttgtg gctgctgctg agaaaaccaa acagggtgtg
     2461 gcagaagcag caggaaagac aaaagagggt gttctctatg taggctccaa aaccaaggag
     2521 ggagtggtgc atggtgtggc aacagtggct gagaagacca aagagcaagt gacaaatgtt
     2581 ggaggagcag tggtgacggg tgtgacagca gtagcccaga agacagtgga gggagcaggg
     2641 agcattgcag cagccactgg ctttgtcgcg aaggaccagt tgggcaagaa tgaagaagga
     2701 gccccacagg aaggaattct ggaagatatg cctgtggatc ctgacaatga ggcttatgaa
     2761 atgccttctg aggaagggta tcaagactat gaacctgaag cctaagaaat atctttgctc
     2821 ccagtttctt gagatctgct gacagatgtt ccatccaagc ttatcgatga taagctgtca
     2881 aacatgagaa tt
//
