LOCUS       sequence-481914-        3073 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Overexpress truncated alpha-Synuclein protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ni X, McGlinchey RP, Jiang J, Lee JC
  TITLE     Structural Insights into alpha-Synuclein Fibril Polymorphism: 
            Effects of Parkinson's Disease-Related C-Terminal Truncations.
  JOURNAL   J Mol Biol. 2019 Sep 6;431(19):3913-3919. doi: 
            10.1016/j.jmb.2019.07.001. Epub 2019 Jul 8.
  PUBMED    31295458
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2019.07.001"; journalName: "J Mol Biol"; date: 
            "2019-09-6- 6"; volume: "431"; issue: "19"; pages: "3913-3919"
COMMENT     Sequence Label: pRK172 asyn(1-103)
FEATURES             Location/Qualifiers
     source          1..3073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(778..796)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        864..968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(969..1037,1038..1829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1187..1206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2000..2588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2489..2508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2742..2759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2774..2913
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2839..2858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2999..3021
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atggatgtat tcatgaaagg actttcaaag gccaaggagg
      121 gagttgtggc tgctgctgag aaaaccaaac agggtgtggc agaagcagca ggaaagacaa
      181 aagagggtgt tctctatgta ggctccaaaa ccaaggaggg agtggtgcat ggtgtggcaa
      241 cagtggctga gaagaccaaa gagcaagtga caaatgttgg aggagcagtg gtgacgggtg
      301 tgacagcagt agcccagaag acagtggagg gagcagggag cattgcagca gccactggct
      361 ttgtcaaaaa ggaccagttg ggcaagaatt gagaaggagc cccacgggca agaattgaga
      421 aggagcccca cgttgggcaa gaattgagaa ggagccccac aggaaggaat tctggaagat
      481 atgcctgtgg atcctgacaa tgaggcttat gaaatgcctt ctgaggaagg gtatcaagac
      541 tatgaacctg aagcctaaga aatatctttg ctcccagttt cttgagatct gctgacagat
      601 gttccatcca agcttgaatt ccgatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggataa ttcttgaaga cgaaagggcc
      781 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      841 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      901 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      961 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1021 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1081 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1141 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1201 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1261 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1321 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     1381 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1441 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1501 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1561 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1621 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1681 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1741 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1801 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1861 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     2161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2701 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2761 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2821 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     2881 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     2941 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3001 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggatctc
     3061 gatcccgcga aat
//
