LOCUS       sequence-481921-        3050 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Overexpress truncated alpha-Synuclein protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ni X, McGlinchey RP, Jiang J, Lee JC
  TITLE     Structural Insights into alpha-Synuclein Fibril Polymorphism: 
            Effects of Parkinson's Disease-Related C-Terminal Truncations.
  JOURNAL   J Mol Biol. 2019 Sep 6;431(19):3913-3919. doi: 
            10.1016/j.jmb.2019.07.001. Epub 2019 Jul 8.
  PUBMED    31295458
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2019.07.001"; journalName: "J Mol Biol"; date: 
            "2019-09-6- 6"; volume: "431"; issue: "19"; pages: "3913-3919"
COMMENT     Sequence Label: pRK172 asyn(1-122)
FEATURES             Location/Qualifiers
     source          1..3050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(649..667)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      663..710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(755..773)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        841..945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(946..1014,1015..1806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1164..1183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1977..2565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2466..2485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2719..2736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2751..2890
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2816..2835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2976..2998
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atggatgtat tcatgaaagg actttcaaag gccaaggagg
      121 gagttgtggc tgctgctgag aaaaccaaac agggtgtggc agaagcagca ggaaagacaa
      181 aagagggtgt tctctatgta ggctccaaaa ccaaggaggg agtggtgcat ggtgtggcaa
      241 cagtggctga gaagaccaaa gagcaagtga caaatgttgg aggagcagtg gtgacgggtg
      301 tgacagcagt agcccagaag acagtggagg gagcagggag cattgcagca gccactggct
      361 ttgtcaaaaa ggaccagttg ggcaagaatg aagaaggagc cccacaggaa ggaattctgg
      421 aagatatgcc tgtggatcct gacaattgag cttatgaaat gccgtggatc ctgacaattg
      481 agcttatgaa atgccttctg aggaagggta tcaagactat gaacctgaag cctaagaaat
      541 atctttgctc ccagtttctt gagatctgct gacagatgtt ccatccaagc ttgaattccg
      601 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      661 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      721 gaactatatc cggataattc ttgaagacga aagggcctcg tgatacgcct atttttatag
      781 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     1201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     1501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2761 tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa
     2821 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     2881 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     2941 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3001 ttcaccgtca tcaccgaaac gcgcgaggca ggatctcgat cccgcgaaat
//
