LOCUS       sequence-481941-        5219 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A segment of the Phi-X174 phage genome was inserted into the p15A 
            plasmid . The genome segment can be stably replicated in plasmid and
            can be genetically modified at the plasmid level.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Zhang H, Zhu R, Wang Z, He R, Zhang Y, Luan J, Yan Y, Zhang Y, Wang 
            H
  TITLE     Programming virulent bacteriophages by developing a multiplex genome
            engineering method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p15A-Phi-X174-F2
FEATURES             Location/Qualifiers
     source          1..5219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(155..700)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(235..254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(814..908)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(929..1588)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1503..1522
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1589..1691)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctagggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
       61 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
      121 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
      181 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
      241 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
      301 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
      361 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
      421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
      481 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
      541 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
      601 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
      661 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca
      721 gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
      781 ccccatacga tataagttgt tactagtgct tggattctca ccaataaaaa acgcccggcg
      841 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
      901 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
      961 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     1021 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     1081 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     1141 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     1201 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     1261 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     1321 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
     1381 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     1441 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     1501 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     1561 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     1621 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1681 cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc ttaagaccca ctttcacatt
     1741 taagttgttt ttctaatccg catatgatca attcaaggcc gaatagtata catggaaggc
     1801 gctgaattta cggaaaacat tattaatggc gtcgagcgtc cggttaaagc cgctgaattg
     1861 ttcgcgttta ccttgcgtgt acgcgcagga aacactgacg ttcttactga cgcagaagaa
     1921 aacgtgcgtc aaaaattacg tgcagaagga gtgatgtaat gtctaaaggt aaaaaacgtt
     1981 ctggcgctcg ccctggtcgt ccgcagccgt tgcgaggtac taaaggcaag cgtaaaggcg
     2041 ctcgtctttg gtatgtaggt ggtcaacaat tttaattgca ggggcttcgg ccccttactt
     2101 gaggataaat tatgtctaat attcaaactg gcgccgagcg tatgccgcat gacctttccc
     2161 atcttggctt ccttgctggt cagattggtc gtcttattac catttcaact actccggtta
     2221 tcgctggcga ctccttcgag atggacgccg ttggcgctct ccgtctttct ccattgcgtc
     2281 gtggccttgc tattgactct actgtagaca tttttacttt ttatgtccct catcgtcacg
     2341 tttatggtga acagtggatt aagttcatga aggatggtgt taatgccact cctctcccga
     2401 ctgttaacac tactggttat attgaccatg ccgcttttct tggcacgatt aaccctgata
     2461 ccaataaaat ccctaagcat ttgtttcagg gttatttgaa tatctataac aactatttta
     2521 aagcgccgtg gatgcctgac cgtaccgagg ctaaccctaa tgagcttaat caagatgatg
     2581 ctcgttatgg tttccgttgc tgccatctca aaaacatttg gactgctccg cttcctcctg
     2641 agactgagct ttctcgccaa atgacgactt ctaccacatc tattgacatt atgggtctgc
     2701 aagctgctta tgctaatttg catactgacc aagaacgtga ttacttcatg cagcgttacc
     2761 atgatgttat ttcttcattt ggaggtaaaa cctcttatga cgctgacaac cgtcctttac
     2821 ttgtcatgcg ctctaatctc tgggcatctg gctatgatgt tgatggaact gaccaaacgt
     2881 cgttaggcca gttttctggt cgtgttcaac agacctataa acattctgtg ccgcgtttct
     2941 ttgttcctga gcatggcact atgtttactc ttgcgcttgt tcgttttccg cctactgcga
     3001 ctaaagagat tcagtacctt aacgctaaag gtgctttgac ttataccgat attgctggcg
     3061 accctgtttt gtatggcaac ttgccgccgc gtgaaatttc tatgaaggat gttttccgtt
     3121 ctggtgattc gtctaagaag tttaagattg ctgagggtca gtggtatcgt tatgcgcctt
     3181 cgtatgtttc tcctgcttat caccttcttg aaggcttccc attcattcag gaaccgcctt
     3241 ctggtgattt gcaagaacgc gtacttattc gccaccatga ttatgaccag tgtttccagt
     3301 ccgttcagtt gttgcagtgg aatagtcagg ttaaatttaa tgtgaccgtt tatcgcaatc
     3361 tgccgaccac tcgcgattca atcatgactt cgtgataaaa gattgagtgt gaggttataa
     3421 cgccgaagcg gtaaaaattt taatttttgc cgctgagggg ttgaccaagc gaagcgcggt
     3481 aggttttctg cttaggagtt taatcatgtt tcagactttt atttctcgcc ataattcaaa
     3541 ctttttttct gataagctgg ttctcacttc tgttactcca gcttcttcgg cacctgtttt
     3601 acagacacct aaagctacat cgtcaacgtt atattttgat agtttgacgg ttaatgctgg
     3661 taatggtggt tttcttcatt gcattcagat ggatacatct gtcaacgccg ctaatcaggt
     3721 tgtttctgtt ggtgctgata ttgcttttga tgccgaccct aaattttttg cctgtttggt
     3781 tcgctttgag tcttcttcgg ttccgactac cctcccgact gcctatgatg tttatccttt
     3841 ggatggtcgc catgatggtg gttattatac cgtcaaggac tgtgtgacta ttgacgtcct
     3901 tccccgtacg ccgggcaata atgtttatgt tggtttcatg gtttggtcta actttaccgc
     3961 tactaaatgc cgcggattgg tttcgctgaa tcaggttatt aaagagatta tttgtctcca
     4021 gccacttaag tgaggtgatt tatgtttggt gctattgctg gcggtattgc ttctgctctt
     4081 gctggtggcg ccatgtctaa attgtttgga ggcggtcaaa aagccgcctc cggtggcatt
     4141 caaggtgatg tgcttgctac cgataacaat actgtaggca tgggtgatgc tggtattaaa
     4201 tctgccattc aaggctctaa tgttcctaac cctgatgagg ccgtccctag ttttgtttct
     4261 ggtgctatgg ctaaagctgg taaaggactt cttgaaggta cgttgcaggc tggcacttct
     4321 gccgtttctg ataagttgct tgatttggtt ggacttggtg gcaagtctgc cgctgataaa
     4381 ggaaaggata ctcgtgatta tcttgctgct gcatttcctg agcttaatgc ttgggagcgt
     4441 gctggtgctg atgcttcctc tgctggtatg gttgacgccg gatttgagaa tcaaaaagag
     4501 cttactaaaa tgcaactgga caatcagaaa gagattgccg agatgcaaaa tgagactcaa
     4561 aaagagattg ctggcattca gtcggcgact tcacgccaga atacgaaaga ccaggtatat
     4621 gcacaaaatg agatgcttgc ttatcaacag aaggagtcta ctgctcgcgt tgcgtctatt
     4681 atggaaaaca ccaatctttc caagcaacag caggtttccg agattatgcg ccaaatgctt
     4741 actcaagctc aaacggctgg tcagtatttt accaatgacc aaatcaaaga aatgactcgc
     4801 aaggttagtg ctgaggttga cttagttcat cagcaaacgc agaatcagcg gtatggctct
     4861 tctcatattg gcgctactgc aaaggatatt tctaatgtcg tcactgatgc tgcttctggt
     4921 gtggttgata tttttcatgg tattgataaa gctgttgccg atacttggaa caatttctgg
     4981 aaagacggta aagctgatgg tattggctct aatttgtcta ggaaataacc gtcaggattg
     5041 acaccctccc aattgtatgt tttcatgcct ccaaatcttg gaggcttttt tatggttcgt
     5101 tcttattacc cttctgaatg tcacgctgat tattttgact ttgagcgtat cgaggctctt
     5161 aaacctgcta ttgaggcttg tggcatttct actctttctc aatccccaat gctgtatac
//
