LOCUS       sequence-481979-       14673 bp    DNA     circular SYN 05-AUG-2025
DEFINITION  Express TNIK-NanoLuc Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14673)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TNIK-NanoLuc Fusion Vector
FEATURES             Location/Qualifiers
     source          1..14673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     502..521
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(607..654)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(669..1026)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      683..818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        835..1026
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     983..1003
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    1271..1392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1308..1327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1362..1381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1429..1447
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1535..2047)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6161..6180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6162..6180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6222..6241)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          6224..6356
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(6492..6695)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6521..6541)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6696..7075
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7520..7537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7654..8199)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7734..7753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    14342..14390
                     /note="synthetic polyadenylation signal"
     CDS             complement(join(14454..14673,1..575))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14565..14584)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
       61 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
      121 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
      181 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
      241 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
      301 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
      361 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
      421 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
      481 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
      541 ggagaacctg cgtgcaatcc atcttgttca atcatgatct gcctcctggg tttagttcct
      601 caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt caacagttaa
      661 ttaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
      721 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      781 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
      841 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      901 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      961 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg
     1021 cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca tggtgctgcg
     1081 cagatccgcg tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1141 ggaaattgta agcgttaata ttttgttaaa attcgatttt accacatttg tagaggtttt
     1201 acttgcttaa aaaaacctcc cacatctccc cctgaacctg aaacataaaa tgaatgcaat
     1261 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1381 caatgtatct tatcatgtct gctcgaagcg gccgccccaa ggggttatgc tagttattgc
     1441 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatcagct
     1501 tgcatgcctg caggtcgact ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
     1561 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
     1621 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
     1681 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
     1741 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
     1801 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
     1861 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
     1921 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
     1981 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
     2041 tgaagacgcc gctcgagccg agagaaaccc agttcatcat ggaatttctg ttgagggtca
     2101 tgaaaaacac ttggctactt cctccagatc gcacggatgc aaaaaatacc ttatcatttc
     2161 tttcacatag aaactttaac ctttgagctc gcttatgcat aaatactcca tccaaatgtc
     2221 ctgtttccac tgaccggatc tcaatagctt tctcgcccca gcccattatc tgattggaat
     2281 gaatgtaggc cacagacgtg ggcatttctc cccattggag caccacatcc ttagttatcc
     2341 ggccataggt gtttacatac accccctcat cctcatagca aacaagcatt tccattccat
     2401 ctgttttagg caagatgaca atagcatgag gagtgatatt gccctgaata tgagatggta
     2461 tgtagatatc ataagagttt cctgaatcaa catcaattac atggaaacca gtgtgtgaac
     2521 caaaaataac ctttaatctt tgaccttctt ctaccgtgag atcaactagc agaggcttgt
     2581 gctggagatc tgcaaaagac ttaaatgcca tgaatttatg atacggttta ggagcccaag
     2641 catatatttc cacagcattc tttaaggcaa tcaccaaaaa tttgatcctt tcatatttaa
     2701 caactttata atgtatacag ccttccaagt ccccaacagt gatccagcct tgtttctttt
     2761 ctacttctgg gtcattatgt agtattctgt ttcttaacca tgaaagatag taaactcgta
     2821 gcttattctt ctttcctgaa attgtcacaa ggacattcag tccctctagc acatccatct
     2881 gctgaaatcg cctccggttg atcagattat agactttgcc ttgcccactt cggtccaaaa
     2941 gcatcaggcc attttcagtc cccaccagaa ggtttacacc ccacagagct gcacaaagta
     3001 tttctgagtt gaatcgtttc ttgtattttc tgatttctgg tgtgtcgcta tgaggccgaa
     3061 tgttggttgg gtttacattt accaccgaaa tctttcttgc ttcattgagt ttggcctgtt
     3121 cttgcctaag aagttcgcta gtaaacagag ctgcggctga tgattcctca tcctcttcat
     3181 cttcatcagt gggagacgtc tggtatactc tggggtccac aaagggggtg aaggaggctt
     3241 tggtgctgct ccccatgcca tattcagtcc cagagtccat ctcctgggaa tgggttgaga
     3301 cgcgccccag tccctcagtc ggggttccag ctggagaatg gctctgctgc accaggtcag
     3361 ggaggttgat gtggccagca aagccattgc tgtcactgtg gccagatcgc ttcttctctc
     3421 cagacgtctc tctaatcatc aaggttcctt ctcttgaaat actgccgctg aaactgtccg
     3481 catgagaggt ctccagccca tgcgtcccca ccattcccac attgtactgc tcgttgctgc
     3541 ctggagctcc tgttggtatc agtctgggta tgtcgctgac agccactgtc ccatcatggg
     3601 tctcgctctc tccatcttcc tcctcttcct cgctactttc tgactcctca ctggaggagg
     3661 agtaatcagt caccttcttc attgggcggt ttgtttcttc aatccggagt tctcttagtt
     3721 ctttggctaa tgccgtcaga tcctcatcta tagctttttt gtagctagct ggtcgactgg
     3781 gccgggtaat gtccctggat tcttctggtt tcaccttggc aggctcatgg ggaagcacag
     3841 gtgatccttc tgacttactg ttggctcgaa ctctggtgcg ttcactggat cctgcttgtg
     3901 atccaggctg ggagcctcct tgggagctgg gctgggagct aggggtgctg gagctggagg
     3961 aactgccact gctggtcctc tgcaaggggc tctccaagat gggctcagtt ctccggagat
     4021 cagggttgct tgctctgatg ggttgagatc ctagtctggg tcccagagca ctaccattcc
     4081 caggagaatt ctttctggct aatgctgggg atatagaagt tgttctttga ggcacctttg
     4141 gtggaatgtc ttcttcctgt cgtaaccaag agcttcggtc aaacttttca atgcgagtgg
     4201 ggagaggagg attttccgag gtgggatctg agttctggcg tggcatctcc acgcggtggg
     4261 aggtcacgtt cagagcctcc tgaaacccag agaggccctt tgtgggctgc tcgtgcactg
     4321 actgggaggc ggtcaaggca ggtccctggg attttacagc taccagatgt gggatctggg
     4381 gatcgactgg tctgagcatg gggggtgttc gagcaggctg aactccacta atgctgaagg
     4441 actccgacct tgggggcagg ttggggtcag atatcctgtt ggcaaccttg tgaggcatgg
     4501 caggggaact ttgccggttg agccttgacc gttcttctac ctccttggcc catgctggct
     4561 tctcactagg actcattcct tctttgtaat ggtacagtgg cttcttctcc acaggcctct
     4621 gctcctgccg ctgatgctga agggaaacta agtagtctct ttcttgcttt agctgcctct
     4681 gcagtctttc tgcttgtctc tgttcttcca attgtttgcg cttatattcc agaagtagag
     4741 cttgttcatg cagtagctgc tgctgcaaga tctctaactg tctctgctcc tcctctaact
     4801 gtcgcctgat gtattcctgt tcatgctccg cacgcctcct ctcctcctcc cggcgcatct
     4861 gctcctcata gtgccggcgc tgctccctct cctgctgctt ccgcagctcc ttctctcgcc
     4921 tttgttgctc ctccagccgc cgcctctgct ctttctgctc ctcgatgcgc ttctgacgct
     4981 cggccagcag ctgccgcttg tgctcctcat tctcccgctg ctgctgctcc agctgctgcc
     5041 tccgtagggc ctcagaacgc tccttgttgg ccagctgcag cctcagaaag tccctccgca
     5101 gcgtcgactc ccctggcaga ttcaggatgg agctgggctc tcctgagtca ttctcctcct
     5161 cttcttcctc acttccactg tactcatact ctgtctcatc tttttctcct cgcttcttct
     5221 ttgttctatc aatatggtcc ttgagttgaa tgcggacctg tcgctcatta ggttggtctc
     5281 gtataaatgg atgcttcatc aattgttctg ttgctggtcg ctggctgtga ttctttacca
     5341 agcagctctc aataaatgac tggaattttt ttgaccactt cttagacttc agccgaggcg
     5401 ctggattccg ggggatgagg aagagagctc tcatggggtg catgtcacag agagggggag
     5461 caccttctgc catttcaatg gcggtgatac ccaaagacca caagtcactc ttgaaatcat
     5521 atgtggcatc tgggttttca tcacaggcaa taacttctgg tgccatccag tagggagttc
     5581 caatgaaagt attcctcctg cccactgttc gatcaagctg agcactgact ccaaagtcca
     5641 ctagtttaac ttctgcattt tcagtcagca agacattttg ccctttaata tctcgatgaa
     5701 tcactttatg ctggtgcagg tgactcagcc cccgtaagat ttccctgcag atgtatgcaa
     5761 tccactcctc tttcaacgtg ttaccttttg tgttcttgat caggtcggtg acagagccag
     5821 caccacaaaa ctccatcacc aaccaaagtt ggtcatccat gcctggtggg ttctttttga
     5881 taaaagcacc atagtatgta gcaatattcc ggtgatgaga atatttcttc aacatgttaa
     5941 tttcttgttt gatttcttcc tcttcatccc ctgtgacatc cataaccttg atggctgcaa
     6001 gctggcccgt tttgacatga cgacccttat aaacttgccc gtatgttcca tttccaacaa
     6061 gttccaccaa ttcaaagatc cctgcagggt ccctcagagc cgagagatct atttcatcca
     6121 ggcttcgagc cggggagtcg ctcgccatgg cgatcgctag ccctatagtg agtcgtatta
     6181 atactctagc cttaagagct gtaattgaac tgggagtgga cacctgtgga gagaaaggca
     6241 aagtggatgt cagtaagacc aataggtgcc tatcagaaac gcaagagtct tctctgtctc
     6301 gacaagccca gtttctattg gtctccttaa acctgtcttg taaccttgat acttacctgc
     6361 ccagtgcctc acgaccaact tctgcagctt aagttcgaga ctgttgtgtc agaagcactg
     6421 actgcgttag caatttaact gtgataaact accgcaataa agcttctagt gatctgacgg
     6481 ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
     6541 gtcaacgggg cggggttatt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
     6601 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
     6661 ccacgcccat tggtgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
     6721 tagatgtact gccaagtagg aaagtcccgt aaggtcatgt actgggcata atgccaggcg
     6781 ggccatttac cgtcattgac gtcaataggg ggcggacttg gcatatgata cacttgatgt
     6841 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     6901 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     6961 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     7021 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgccaacat
     7081 ggcggtcata ttggacatga gccaatataa atgtacatat tatgatatag atacaacgta
     7141 tgcaatggcc aatagccaat attgatttat gctatataac caatgaataa tatggctaat
     7201 ggccaatatt gaagatctcg atcctctacg ccggacgcat cgtggccgga gtactgcggt
     7261 ttatctcgat ggctacgagg gcagacagta agtggattta ccataatccc ttaattgtac
     7321 gcaccgctaa aacgcgtaat ctcatgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     7381 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     7441 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     7501 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     7561 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     7621 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     7681 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     7741 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     7801 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     7861 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     7921 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     7981 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     8041 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     8101 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     8161 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct taggggtttg
     8221 agggccaatg gaacgaaaac gtacgtttat ggtataactt attgattata atacagtata
     8281 caaaggtccc ttttcaggga ccctttcgta tgttgtagct gattttattt tcttcttttc
     8341 ttttgggtgc ttttaatagc catatatgat gtttcatata aagttaaaag tttccccatc
     8401 tgtctatcta tagcgtcatg tttttcagga tttcttaatt tctgcagcag tttagatata
     8461 tatttcgcag gtaactttat tattaatgaa ttatgggatt gatacatttc ctttgtccaa
     8521 tctaaatctt ttcctgattt caatacctca ctctcatctc tattaattat aaaaccgagc
     8581 tttccatacg gacctgttaa atatgattgt aattgtctat actcagatgg acctaattct
     8641 tcaaaatttt tagcatcaaa aacaacttgt cttgttttat agtcctccaa tactcgtttc
     8701 caaaaatcag atttgccacc attggtgcct ataatatctc gtctctgaac tgcgttacca
     8761 tttggatggg acttgatgtc tgttaggtgg gatgcaaata ctattcttag tgcgtctaaa
     8821 caccattgtt caaattcagt ggcaccttca tttcctatcg gtatctgatc tagatgagta
     8881 gttatttgac ctattgtttt atttctaatg gctgaattat ctgaaataat attaatatca
     8941 tattcgtcat ttatctcctc tgcttcctct ggagcaagtg cattacgatt tagattcaaa
     9001 ccaagccagt aacatggatg aattaataat ttctcattac tttcaaaacc tttatctgga
     9061 gttcgcccgt catggcaaaa tgaataggat gaggtgtttt tatcacgaat accaacaaat
     9121 cctacactat acaaactttg gagaattcca cttgccttta acaactgtat ttcagacgtt
     9181 attttaggat ctccattttc ttcgattaat tcgaaagatg cttctatttt ttttaagcac
     9241 gtataaactg ttaattcagg ttcaatgcta cgaaatgcac tagttataac ctgtattgaa
     9301 ggaaagatct tctgatactc tttccagaga tcttcaagtc tggccatgga aattgacttg
     9361 gctgcatatt ctaggtcagt gtttatgata gtttctctat tctctctgaa tgcggaaaaa
     9421 aaagcttcat tcaacaatga tagtaaatcc ctgggccggt aaagggtaaa ttgcaaacat
     9481 cgcttaaaac cattcctccc tttaagatca tccgctgtgc atctatccca aactcgttga
     9541 tctttctcaa tatctagctt aaatgctact ttcattcttt tagctgacag cattaggagt
     9601 tgtgcccagt cccaatgcaa ccttatgact tgaccctcta tatttctcga gtaatcagga
     9661 tcttcctttg atagcgacct aaatatatta tcccttaaaa aaattattgg acgaatgcat
     9721 tttgcttttt gatttaattc aatagatgca tatgctagac ctgcaatgat tccaattcct
     9781 atattatccg gttcatacgc ctcatctagc ttatccatta atatgacaac tttcctgtct
     9841 gagcgttcaa gaagtgatac tatattattt tctatttctg agatattcaa attgaattga
     9901 agatcaccaa ttgattcttc tgggttattt tcatctaaat actcctttgc gacaagccta
     9961 cactttctta aaatgtcacc ttgtgcagaa ttccattttt tcaaatgttc attcaacaat
    10021 gtttctgatg atatttgaga tgacaatttg taatgagatg atatatatga tgctatctcc
    10081 attagcatag cgtatcgcca taatagtctt gttgctgccc ttgctaaatt aaatgatcct
    10141 gtaaatggtt tcaacattga tctgaaacca ataatttgag aatcgtctgg tgagaaactc
    10201 aggattaata tttttttgtc tttcttccaa tgctcattta gctgaataaa taaagcactt
    10261 ttacctgtcc ctcgtctacc aacaacaatg gtcctgtcat cagtttcaat tagagtccta
    10321 aagtcagcag tttcaatgaa agcattactc aacatctttt tatcattttc tgctgtcgta
    10381 tcaccaaacg gattagactt tgaagtaata ttcaattcca tattcaacct tttatgttag
    10441 ttgctttcat ttattacttt atatactgtt gaacgagcaa tattcattgt ttttgatata
    10501 tgtgaggcac ctaacccctg ttgccacata tttaatactg catctctatc tatttttctt
    10561 tttctaccaa aaacaactcc ttttgccatt gcctcttggc gaccttcatt ggtacgctct
    10621 agtattctct gtcgttctgc ctgggccact gcagatagaa tagtgacaac cattttaccc
    10681 atctccccat cggtactgat tccgtcatca ataaaccgaa tggatacacc ttgggcgtca
    10741 aactctttta ttaactggat catgtcagca gtatcgcgcc caaggcggtc aagtttcttc
    10801 accaagatga cgtcaccttc ctccaccttc atcctcagca agtccagccc tttccgatcg
    10861 cttgaactgc ccgatgcctt gtcagtaaag atgcgatttg ctttcacgcc tgcgtctttg
    10921 agtgcccgaa cctgaatatc gagagattgc tggctggttg atacccgtgc gtaaccaaaa
    10981 agtcgcataa aaatgtatcc taaatcaaat atcggacaag cagtgtctgt tataacaaaa
    11041 aatcgattta atagacacat taacagcact gtttttatgt gtgcgataat ttataatatt
    11101 tcggacggtt gcaaaattgt taatgaataa ctgtcaggta gggagcctga tatgcctgtc
    11161 gactttctaa cctctgacca aaaacagaat tacggttgtt atgctgcaga acctaatgac
    11221 gtgcaactgg cgcgctattt tcatcttgat gaacgggatc tggccttcat taaccaacga
    11281 cggggcaaac ataataggct gggcattgcg cttcagctca ccacagcccg ttttctggga
    11341 acatttctga cggatttaac tcaggttctg cctggtgttc aacattttgt cgcggtacag
    11401 cttaatatcc accgtccaga agttctctcc cgctatgctg aacgggacac tacccttaga
    11461 gaacatactg cattaattaa ggaatattac ggctatcatg aatttggtga ttttccatgg
    11521 tctttccgcc tgaagcgtct gctatatacc cgggcgtggc tcagtaatga gcgaccgggt
    11581 ctgatgtttg attttgccac tgcatggttg cttcaaaata aggtattact gcccggagca
    11641 accacactag tacgtctcat cagtgaaatt cgtgaaaggg caaatcagcg gctgtggaaa
    11701 aagctggccg cactgccgaa caaatggcag gcagctcaag tgatggagct tctggtcatt
    11761 ccggaaggtc agcgtgtatc agcactggaa cagttgaaaa aaggccctgt tacagtcagt
    11821 ggacctgcgt ttaatgaagc gctggaacga tatatccgat tacgaagtct tgagttttcc
    11881 cgactgaact tttccggtct gcctgccatt caactgcgta atctggctcg ttatgctggc
    11941 atggcgtcgg taaaatatat cgctcgaatg ccacagcaga gaaagcttgc tgtacttact
    12001 gcattcgtta aagcacagga aataacggca ttagacgatg ccgttgatgt gcttgatatg
    12061 ctaattctgg acattatccg cgaagcaaag aaaaccgggc aaaaaaaaag actcaggaca
    12121 ctgaaagatc ttgatcaggc cgcattgtta ctggcgcggg catgtgcatt gttgctggat
    12181 gataatacag atgtcccaga tctcaggcag gttatcttca agtgcgtacc caaaaacaga
    12241 ctggcagaat ctgtaagcaa ggttaatgaa cttgctcgtc cacagaacaa taatttccat
    12301 gacgaaatgg ttgagcagta cggtcgggtt aaacgttttc ttccggcggt gttgcgggac
    12361 ctgcatttcc gtgcggcacc ggcaggtgaa catgtactgg ctgcgattca ttatctggca
    12421 gaactgaatg gttcgaaaaa gcgcatcctt gatgatgcgc ctgaacatat tatcaccggt
    12481 ccctggaaac gcctcgtata cgatgcggag ggacggatac agcgtgcagg ttattcacta
    12541 tgtttgctgg aacgccttca ggatgcactg cgccgccggg acatctggct tgaaaacagt
    12601 gatcgctggg gagatcctcg cgagaagttg ttgcaaggtg aagagtggca gactcagcgt
    12661 attcctgtct gtcgggcact gggacatcct gtcgatggac gtaaaggtgt gcaacaactg
    12721 gctattcagc tggatgagac ctggaaagcc gtggcatcac gatttgaaaa gaatgcggaa
    12781 gttcatatct gtaatgaagg taaatatcca tccctgacta tcagttgtct ggagaaacag
    12841 gaagagccac catcattgct tcgtctaaat aatcggatca aacagctact cccaccggta
    12901 gatttaacgg aactgttact tgagatagat gcccagacag gatttacaca tgagtttgcg
    12961 catgtcagtg aatctggtgc tcgagcgcaa gatttgcaca tcagtttatg tgcggtattg
    13021 atggctgaag cctgtaatat cggactggaa ccgctgataa agcacaatat accagcactg
    13081 acccgccatc ggctcagttg ggtgaaacag aattaccttc gtgcagaaac gctggtcagc
    13141 gccaatgccc gcctggttga ttttcagtcc acactggagc ttgctggtcg ttggggaggt
    13201 ggagaagtgg catcagctga cggcatgcgc tttgtcacac cagtgaagac catcaactca
    13261 ggatctaaca gaaaatattt tggttctgga cgaggcatca cctggtataa cttcgtatct
    13321 gatcagtact ctgggttcca tggcattgtg gtacccggta cattacggga ttcgattttt
    13381 gtactggaag gacttcttga gcagcagaca gggctgaatc cagttgaaat catgacagac
    13441 actgcgggta gcagcgatat tattttcggt ctgttctggc tactggggta tcagttttcc
    13501 ccccggcttg ccgatgcagg tgaggcggtg ttctggcggg tgaataaatc ggcaaactac
    13561 ggagtactgg ataagttggc ccgaggatat gcagatctgt caaaagcgga gagtcagtgg
    13621 gatgagatga tgcgaaccgc tggttcgctg aagttgggta caattcatgc gtcagaactc
    13681 attcgctctt tactgaaaag ctcgcgccca tcaggactgg cacaggcaat tatggaagta
    13741 gggcgtgtaa acaaaacgct atatctcctc aattatattg atgatgaaga ttatcgtcgg
    13801 cggatcctga cgcagcttaa tcgaggagaa ggccgccacg ctgtggctcg tgcgatttgt
    13861 tacggacaac gcggagagat cagaaagcgt tatcgtgaag ggcaggaaga tcaactgggg
    13921 gcgctgggcc tggttactaa tgcagtggtt ctgtggaaca cgctctatat ggaggaagcg
    13981 ttgagctgga tgcgccgtaa tggcgaagaa attatagatg aagatatcgc tcggctatct
    14041 cccctgatgc acgggcatat caatatgttg ggccattata cattcacgtt gccagaggat
    14101 attttaaaag gggaactgag agctctaaat ttaaatataa acaacgaatt atctccttaa
    14161 cgtacgtttt cgttccattg gccctcaaac ccctcttatt aatcagataa aatatttcta
    14221 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
    14281 gttgtaattc tcatgtttga cagcttatca tcgcatgagc ggatccttat cgctatcgat
    14341 tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt gcggccatcg
    14401 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
    14461 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
    14521 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
    14581 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
    14641 aagcggccat tttccaccat gatattcggc aag
//
