LOCUS       sequence-482060-       10918 bp    DNA     circular SYN 05-AUG-2025
DEFINITION  Over-express Sc rev3-FLAG (Sc Pol zeta subunit) in yeast 
            (integrated).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10918)
  TITLE     O'Donnell lab plasmids 2025
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Sc rev3-FLAG-pRS402/GAL
FEATURES             Location/Qualifiers
     source          1..10918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        161..536
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             537..2252
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     rep_origin      complement(2457..2912)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2594..2615)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2806..2825
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3038..3060
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3052..3069
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3053..3069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3079..3098
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3079..3097
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3122..3138
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3123..3139
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3167..3831
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    3383..3500
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3795..3818
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             8353..8418
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(8458..8478)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8458..8476)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8497..8513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8497..8513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8510..8532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8521..8537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8545..8551,8552..8569,8570..8575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8590..8611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8728..8745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8899..9487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8979..8998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9658..10449,10450..10518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10281..10300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10519..10623)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10691..10709
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10747..10769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10869..10888
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
       61 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
      121 gcaaaatccc ttataaatca aaagaataga tctgaattaa ttcttgaata atacataact
      181 tttcttaaaa gaatcaaaga cagataaaat ttaagagata ttaaatatta gtgagaagcc
      241 gagaattttg taacaccaac ataacactga catctttaac aacttttaat tatgatacat
      301 ttcttacgtc atgattgatt attacagcta tgctgacaaa tgactcttgt tgcatggcta
      361 cgaaccgggt aatactaagt gattgactct tgctgacctt ttattaagaa ctaaatggac
      421 aatattatgg agcatttcat gtataaattg gtgcgtaaaa tcgttggatc tctcttctaa
      481 gtacatccta ctataacaat caagaaaaac aagaaaatcg gacaaaacaa tcaagtatgg
      541 attctagaac agttggtata ttaggagggg gacaattggg acgtatgatt gttgaggcag
      601 caaacaggct caacattaag acggtaatac tagatgctga aaattctcct gccaaacaaa
      661 taagcaactc caatgaccac gttaatggct ccttttccaa tcctcttgat atcgaaaaac
      721 tagctgaaaa atgtgatgtg ctaacgattg agattgagca tgttgatgtt cctacactaa
      781 agaatcttca agtaaaacat cccaaattaa aaatttaccc ttctccagaa acaatcagat
      841 tgatacaaga caaatatatt caaaaagagc atttaatcaa aaatggtata gcagttaccc
      901 aaagtgttcc tgtggaacaa gccagtgaga cgtccctatt gaatgttgga agagatttgg
      961 gttttccatt cgtcttgaag tcgaggactt tggcatacga tggaagaggt aacttcgttg
     1021 taaagaataa ggaaatgatt ccggaagctt tggaagtact gaaggatcgt cctttgtacg
     1081 ccgaaaaatg ggcaccattt actaaagaat tagcagtcat gattgtgagg tctgttaacg
     1141 gtttagtgtt ttcttaccca attgtagaga ctatccacaa ggacaatatt tgtgacttat
     1201 gttatgcgcc tgctagagtt ccggactccg ttcaacttaa ggcgaagttg ttggcagaaa
     1261 atgcaatcaa atcttttccc ggttgtggta tatttggtgt ggaaatgttc tatttagaaa
     1321 caggggaatt gcttattaac gaaattgccc caaggcctca caactctgga cattatacca
     1381 ttgatgcttg cgtcacttct caatttgaag ctcatttgag atcaatattg gatttgccaa
     1441 tgccaaagaa tttcacatct ttctccacca ttacaacgaa cgccattatg ctaaatgttc
     1501 ttggagacaa acatacaaaa gataaagagc tagaaacttg cgaaagagca ttggcgactc
     1561 caggttcctc agtgtactta tatggaaaag agtctagacc taacagaaaa gtaggtcaca
     1621 taaatattat tgcctccagt atggcggaat gtgaacaaag gctgaactac attacaggta
     1681 gaactgatat tccaatcaaa atctctgtcg ctcaaaagtt ggacttggaa gcaatggtca
     1741 aaccattggt tggaatcatc atgggatcag actctgactt gccggtaatg tctgccgcat
     1801 gtgcggtttt aaaagatttt ggcgttccat ttgaagtgac aatagtctct gctcatagaa
     1861 ctccacatag gatgtcagca tatgctattt ccgcaagcaa gcgtggaatt aaaacaatta
     1921 tcgctggagc tggtggggct gctcacttgc caggtatggt ggctgcaatg acaccacttc
     1981 ctgtcatcgg tgtgcccgta aaaggttctt gtctagatgg agtagattct ttacattcaa
     2041 ttgtgcaaat gcctagaggt gttccagtag ctaccgtcgc tattaataat agtacgaacg
     2101 ctgcgctgtt ggctgtcaga ctgcttggcg cttatgattc aagttataca acgaaaatgg
     2161 aacagttttt attaaagcaa gaagaagaag ttcttgtcaa agcacaaaag ttagaaactg
     2221 tcggttacga agcttatcta gaaaacaagt aatatataag tttattgata tacttgtaca
     2281 gcaaataatt ataaaatgat atacctattt tttaggcttt gttatgatta catcaaatgt
     2341 ggacttcata catagaaatc aacgcttaca ggtgtccttt tttaagaatt tcatacataa
     2401 gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2461 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2521 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2581 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2641 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2701 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2761 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2821 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2881 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     2941 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3001 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3061 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc
     3121 ctcgaggtcg acggtatcga taagcttgat atcgaattcc ttgaattttc aaaaattctt
     3181 actttttttt tggatggacg caaagaagtt taataatcat attacatggc attaccacca
     3241 tatacatatc catatacata tccatatcta atcttactta tatgttgtgg aaatgtaaag
     3301 agccccatta tcttagccta aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac
     3361 tgctcattgc tatattgaag tacggattag aagccgccga gcgggtgaca gccctccgaa
     3421 ggaagactct cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg
     3481 cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga
     3541 agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatgaacgaa tcaaattaac
     3601 aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg
     3661 aagcgatgat ttttgatcta ttaacagata tataaatgca aaaactgcat aaccacttta
     3721 actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc
     3781 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccggatcc
     3841 atgtcgaggg agtcgaacga cacaatacag agcgatacgg ttagatcatc ctctaaatca
     3901 gactatttta gaatccagct aaataatcaa gactactata tgtcaaaacc cacctttttg
     3961 gacccatcgc acggtgaaag cctgccctta aaccagtttt cacaggtccc aaacatcagg
     4021 gtgtttggtg ctttacccac agggcatcaa gtattgtgcc acgtccatgg cattctacct
     4081 tacatgttca tcaagtatga tggacaaata actgacacga gtacattgag acaccaaaga
     4141 tgtgcccaag ttcataaaac gctggaagta aaaattaggg catcctttaa aaggaaaaaa
     4201 gatgataaac acgatttagc cggcgacaaa cttggaaatc tcaattttgt cgctgatgtc
     4261 tctgttgtaa agggtatacc attttatggt taccacgttg gctggaatct gttttacaaa
     4321 atttctttgc ttaatccttc ttgtctaagt aggatttcgg aattgattag agatggcaag
     4381 attttcggaa agaaattcga aatttatgaa tcacatattc cttacttgct acaatggacc
     4441 gcagatttta acttatttgg atgttcatgg ataaacgtgg ataggtgtta ttttcgctct
     4501 cctgttttaa atagtatact ggacatagat aagctgacaa ttaatgatga tcttcagttg
     4561 ttactagatc gattttgtga cttcaagtgt aacgtactca gtagaaggga ttttccacgg
     4621 gttggaaatg gattgattga gatagatata ctaccgcaat tcatcaaaaa tagagaaaag
     4681 ttacagcata gagatataca tcacgacttt ttggaaaaac tgggagatat ttctgacata
     4741 cctgtcaaac catatgtatc ctctgctagg gacatgataa atgaacttac catgcaacga
     4801 gaggagttgt ccttgaagga atataaagaa ccaccagaaa caaagcgcca tgtgagtggt
     4861 catcaatggc aatcttctgg agagttcgaa gcgttttata agaaagcaca acataaaaca
     4921 agtacttttg atggtcaaat accaaatttt gagaacttta tagataagaa tcaaaaattc
     4981 tcagccataa atacgcccta tgaagcatta ccgcaattgt ggccaaggct tccacagatt
     5041 gaaataaaca ataatagtat gcaagataaa aagaacgacg accaagttaa tgcatcgttc
     5101 accgaatacg agatatgtgg tgttgataat gagaacgaag gtgtaaaagg aagcaatata
     5161 aagtctcggt cttattcatg gttaccggaa agcattgcgt ctccgaaaga ttctactata
     5221 ctattagatc atcaaacaaa atatcacaat acgataaatt tttcaatgga ttgtgctatg
     5281 acgcaaaata tggcaagtaa acgaaaactt agatcatctg tttctgctaa taaaacatcg
     5341 ttgctgtctc ggaaaagaaa gaaggttatg gctgcgggat tacgctatgg aaaaagagcc
     5401 tttgtttatg gtgagcctcc tttcggttat caagatattc tgaataaatt ggaagacgag
     5461 ggattcccaa aaatagacta taaggaccct ttcttttcga atccagttga tctagaaaat
     5521 aaaccttacg cgtacgcagg taagaggttt gaaataagct cgacacatgt atcaacgaga
     5581 atacccgtcc aatttggggg agagaccgta tcagtttata acaagccaac tttcgacatg
     5641 ttttcctcct ggaaatatgc gttaaaacca cctacgtatg atgctgttca aaaatggtac
     5701 aataaagtac cctcgatggg aaacaaaaaa actgagtctc agataagcat gcacacccct
     5761 catagtaagt ttttatacaa atttgctagt gacgtttctg gaaaacaaaa aagaaaaaaa
     5821 agtagcgttc acgattctct tactcatctg acacttgaga ttcatgcaaa cacgagaagt
     5881 gataaaattc cagaccctgc aatagatgaa gtttccatga ttatatggtg tcttgaagaa
     5941 gaaactttcc ctttagattt ggatatcgct tacgaaggga ttatgatagt ccacaaagct
     6001 tctgaggata gtacatttcc tactaaaatt caacattgta ttaacgaaat tcccgtgatg
     6061 ttctatgaaa gtgaatttga aatgtttgag gctctaactg atttggtatt acttcttgat
     6121 cctgatattc tttccggttt cgaaatacat aatttttcgt ggggttatat aattgaaagg
     6181 tgtcaaaaaa tacatcaatt tgacattgtc agggaacttg ccagagtcaa atgccaaatt
     6241 aagacgaagt tgtcagatac ttggggatat gcccattcct caggaattat gattaccgga
     6301 agacatatga ttaatatatg gagagcgttg aggtccgacg taaatttaac gcaatatact
     6361 attgaaagtg ctgcgttcaa tatcctccat aaacgattac ctcacttttc atttgagtca
     6421 ttaactaata tgtggaacgc taaaaagagc acaaccgaat taaagactgt gttaaattat
     6481 tggttatcaa gagctcagat aaatatacaa ctattaagaa agcaggatta cattgctcga
     6541 aatatcgaac aggcaagatt gataggaata gatttccatt ctgtatatta cagaggatca
     6601 caatttaaag ttgagtcttt tttaatccga atatgcaagt ccgaaagttt tatccttctt
     6661 tctccaggca aaaaggatgt tcgtaagcaa aaagcacttg aatgtgtgcc tttggttatg
     6721 gagccagaat ctgctttcta caaaagtcct ttaattgtgc tggatttcca atcattgtat
     6781 ccatccatta tgattggata caactattgc tattcgacta tgataggaag agtgcgagaa
     6841 ataaacttaa cggaaaataa ccttggagta tctaagtttt cattaccaag aaacatttta
     6901 gctttattaa aaaatgatgt aactatcgct ccaaatggtg ttgtttatgc caagacctct
     6961 gttagaaaat caacgttatc caaaatgtta acagatatcc ttgatgtcag agtgatgata
     7021 aagaaaacaa tgaacgaaat aggtgatgac aacactaccc taaaaaggct tttgaataac
     7081 aaacagttag cactaaaatt attggcgaat gtcacctacg gttatacatc agcttcattt
     7141 tctggacgaa tgccatgctc tgatttagct gatagcattg tacaaacagg cagagaaaca
     7201 ttggagaaag caatagatat tattgagaaa gatgaaactt ggaacgccaa agttgtctat
     7261 ggagacacag atagtttatt tgtatatcta cctggaaaaa ctgctattga ggctttttct
     7321 ataggacatg ctatggcaga aagagttact caaaacaatc caaaaccaat ctttttgaag
     7381 tttgaaaaag tataccatcc ctccatatta attagcaaaa aaaggtacgt aggattttcc
     7441 tatgaaagtc cttcgcagac ccttcctatt tttgatgcta agggtattga aactgttaga
     7501 agggatggta tcccagccca gcagaagatt attgaaaaat gtattcgatt actttttcaa
     7561 accaaagacc tgtcaaaaat aaaaaagtat cttcaaaatg aattttttaa gattcaaata
     7621 ggaaaggtat ctgcccaaga tttttgtttt gcaaaagaag ttaaattagg agcgtataaa
     7681 agcgaaaaga cagcccctgc aggtgccgtg gttgtaaaaa gaagaataaa tgaagaccat
     7741 agagcagaac cccaatacaa ggagcgtata ccttaccttg ttgttaaagg aaagcaagga
     7801 caactgcttc gggaaagatg cgtatcacca gaagagttct tagaaggtga gaatttagag
     7861 ttagattcgg agtattatat aaacaaaatt ctgatacctc ctcttgatag attgttcaat
     7921 ttgattggta taaatgttgg caactgggct caggaaatag taaaatccaa aagggcgagc
     7981 acaactacta caaaagtgga aaacataaca agagtaggaa cttctgcaac atgttgtaat
     8041 tgtggtgaag aattgactaa aatatgttca cttcagttat gtgatgactg tttagagaaa
     8101 agaagcacca caaccttatc atttctcata aagaaattaa agagacaaaa agaataccaa
     8161 acactaaaga ccgtgtgcag gacgtgcagt tatcgttaca cttccgatgc aggcatcgaa
     8221 aatgaccata tagctagtaa atgcaattca tatgactgtc cagtatttta ctctcgtgtc
     8281 aaagcagaaa gatacttgag agataatcaa tctgttcaaa gggaagaagc gctaatatct
     8341 ctaaatgatt gggactacaa agaccatgac ggtgattata aagatcatga catcgactac
     8401 aaggatgacg atgacaagtg agcggccgcc accgcggtgg agctccagct tttgttccct
     8461 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8521 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8581 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8641 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8701 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8761 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8821 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8881 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8941 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9001 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9061 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9121 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9181 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9241 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9301 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9361 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9421 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9481 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9541 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9601 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9661 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9721 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9781 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9841 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9901 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9961 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10021 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10081 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10141 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10201 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10261 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10321 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10381 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10441 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10501 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10561 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10621 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10681 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10741 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10801 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10861 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaata
//
