LOCUS       sequence-482117-        1056 bp    DNA     linear   UNA 03-OCT-2025
DEFINITION  Distribution and localization of human wild-type Munc13-4 in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP Munc13-4
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    31..96
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    220..341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(257..276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     311..330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(348..803)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(485..506)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     697..716
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        830..934
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacggacnc nggcaggacg agctgtacag tccggactca gatctcgagc tcaagcttcg
       61 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
      121 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      181 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      301 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
      361 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
      421 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      481 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      541 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
      601 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
      661 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
      721 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
      781 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      901 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
      961 aatgggtgtc agttaggggn gnggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1021 angcangcat ctcnantnag tcagcaccna tagtcc
//
