LOCUS       sequence-482137-        1175 bp    DNA     linear   UNA 03-OCT-2025
DEFINITION  Calcium binding defective human Munc13-4 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ptwin strep HRV3C Flag Munc13-4 C2AB
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(68..73,74..97,98..133,134..157)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             176..199
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             200..223
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggnanatt agcagacngg ttngtgaacc gtcagatccg ctagcgctac cggtcgccac
       61 catgggcagc gcatggagtc atcctcaatt cgagaaaggt ggaggttctg gcggtggatc
      121 gggaggttca gcgtggagcc acccgcagtt cgaaaaatcc ggaggtgctt ctggtctgga
      181 agttctgttc cagggacccg attacaagga tgacgacgat aagtccggac tcagatctcg
      241 agctcaaatg gcgacactcc tctcccatcc gcagcagcgc cctcccttct tgcgccaggc
      301 catcaagata aggcgccgca gagtcagaga tctacaggat cccccgcccc aaatggcccc
      361 ggagatccag cctccatccc accacttctc ccccgagcag cgggccctgc tctacgagga
      421 cgcactctac actgtcttgc accgcctggg tcatcctgag cccaaccatg tgacggaggc
      481 ctctgagctg ctgcgatacc tgcaggaggc cttccacgtg gagcccgagg agcaccagca
      541 gacactgcag cgggtcaggg agcttgagaa gccaatattt tgtctgaagg caacagtgaa
      601 acaggccaag ggcattctgg gcaaaaatgt cagtgggttc agcaacccct actgcctgct
      661 gggcattgag cagggggtag gtgtgccagg gggcagcccc gggtcccggc atcggcagaa
      721 ggctgtggtg aggcacacca tccccgagga ggagacccac cgcacgcagg tcatcaccca
      781 gacactcaac cccgtctggg acgagacctt catcctggag tttgaggaca tcaccaatgc
      841 gagctttcat ctggacatgt gggacctgga cactgtggan tctgtccgan agaagcttgg
      901 ggagctcacg gatctgcatg ggcttcgcag gatctttaaa aggcccggaa ggacaaangc
      961 cagnacgant ttttggggaa ngtggtttna agctgcagaa ctgccctgcc aaaagaacaa
     1021 tggtacccct ggancccgca ntgaaactac caaacgagcc agngcccctc agttcanttn
     1081 tcataanggn anncnttggc ancgttnaan caataacngg gaactcnctt tgnaaaattt
     1141 gtnccnagtc ccccaaaggg gaaaccttgg gnggt
//
