LOCUS       sequence-482202-       15085 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  EF1-alpha-driven expression of dCas9 fused to miniTurbo with GFP 
            (Adapted from plasmid #153209).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15085)
  TITLE     LncRNA CISTR-ACT regulates cell size in human and mouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dCas9-miniTurbo
FEATURES             Location/Qualifiers
     source          1..15085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3032
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     3537..3553
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3538..3554
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3557..4145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3663..3674
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4072..4092
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4170..4883,4884..4886))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(4173..4883,4884..4886))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4215..4236)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4564..4583
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(4887..4940)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4956..4982)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4992..5012)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5022..5792)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(5793..5834)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(join(5847..7361,7362..7364,7365..7430,7431..7436,
                     7437..9920,9921..9923,9924..9950))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(5847..7430,7431..7433,7434..9920,9921..9923,
                     9924..9950))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5847..9950)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(9960..9980)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9987..10052)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      10052..10061
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10062..11240)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10071..11009
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(10094..10114)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11365..11482
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11780..11824)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12009..12242
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12735..12860
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             12907..13087
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(13105..13303)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(13130..13150)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        13305..13684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     13856..13875
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        13950..14054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14055..14123,14124..14915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc
     3061 atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca
     3121 tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct
     3181 tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     3241 ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag
     3301 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     3361 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     3421 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     3481 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     3541 ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     3601 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     3661 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     3721 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     3781 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     3841 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     3901 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     3961 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     4021 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     4081 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     4141 tgattatcga taagcttgat atcgaattct tacttgtaca gctcgtccat gccgagagtg
     4201 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     4261 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     4321 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     4381 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     4441 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     4501 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     4561 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     4621 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     4681 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     4741 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     4801 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     4861 gtgaacagct cctcgccctt gctcactggg ccaggattct cctcgacgtc accgcatgtt
     4921 agcagacttc ctctgccctc tccactgccg ctagcagcgt aatctggaac atcgtatggg
     4981 taagcggccg ccaccttcct ctttttctta ggtccggatc ccttttcggc agaccgcaga
     5041 ctgatttctc cgcccatcca gggtttgata actccgtcct gttccagcag cagggctccc
     5101 tgtttatcaa tcccccggct aatcccgaaa atctctttgt ccccaatgat cagcttcacg
     5161 ggtctattga tgaagttatc cagcttctcc caccgtgaca gatatggagc caggccttcc
     5221 tgctcgaaca gttccagggc tgcccgcagc tctcggatca gcatagcggc cagagtattc
     5281 ctgtccaggt taatccctgc ttcctgcagt gtgatccagc cctgattgac cacgctttcc
     5341 tccacgcgcc tcatagccac gttaatcccg gcgccaatga cgatctgtgc agcatcgcct
     5401 gttattccgg ccagctccac caggatgcct gccagctttc tatcctgcag atacaggtca
     5461 ttgggccatt tgactcgcac cttgtctgct cccagctttc gcagcgcttc tgccatgaca
     5521 attccgatga ccgggcccag gccgattgct gctggtcccc gcttcaggcg ccaaaacata
     5581 ctcaggtaca ggttagctcc aaaaggagag aaccatttcc tccctctgct tcctctccct
     5641 gcctgctgat attctgcaat gcaagcatcc ccactcttca gctcgccgat tcgatccagc
     5701 aggtactgat tggtggagtc gaccacaggc aggactgcca cgctcccgcc gtccagctgt
     5761 cccagaatct gtttagcgtt cagcagcggg atggtgctgt ccaggcccag cagggggttg
     5821 gggatgggct tgccagaacc ccgcacgtct ccaccgagct gagagaggtc gattcttgtt
     5881 tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt ccaggacctc ctttgtagag
     5941 gtgtaccgct ttctgtctat ggtggtgtcg aagtacttga aggctgcagg cgcgcccaag
     6001 ttggtcagag taaacaagtg gataatgttt tctgcctgct ccctgatggg cttatccctg
     6061 tgcttattgt aagcagaaag caccttatcg aggttagcgt cggcgaggat cactcttttg
     6121 gagaattcgc ttatttgctc gatgatctca tcaaggtagt gtttgtgttg ttccacgaac
     6181 agctgcttct gctcattatc ttcgggagac cctttgagct tttcatagtg gctggccaga
     6241 tacaagaaat taacgtattt agagggcagt gccagctcgt tacctttctg cagctcgccc
     6301 gcactagcga gcattcgttt ccggccgttt tcaagctcaa agagagagta cttgggaagc
     6361 ttaatgatga ggtctttttt gacctcttta tatcctttcg cctcgagaaa gtcgatgggg
     6421 tttttttcga agcttgatcg ctccatgatt gtgatgccca gcagttcctt gacgcttttg
     6481 agttttttag acttcccttt ctccactttg gccacaacca gtacactgta agcgactgta
     6541 ggagaatcga atccgccgta tttcttgggg tcccaatctt ttttgcgtgc gatcagcttg
     6601 tcgctgttcc ttttcgggag gatactttcc ttggagaagc ctccggtctg tacttcggtc
     6661 tttttaacga tgttcacctg cggcatggac aggaccttcc ggactgtcgc gaaatcccta
     6721 cccttgtccc acacgatttc tcctgtttct ccgtttgttt cgataagtgg tcgcttccga
     6781 atctctccat tggccagtgt aatctcggtc ttgaaaaaat tcataatatt gctgtaaaag
     6841 aagtacttag cggtggcctt gcctatttcc tgctcagact ttgcgatcat tttcctaaca
     6901 tcgtacactt tatagtctcc gtaaacaaat tcagattcaa gcttgggata ttttttgata
     6961 agtgcagtgc ctaccactgc attcaggtag gcatcatgcg catggtggta attgttgatc
     7021 tctctcacct tataaaactg aaagtccttt ctgaaatctg agaccagctt agacttcaga
     7081 gtaataactt tcacctctcg aatcagtttg tcattttcat cgtacttggt gttcatgcgt
     7141 gaatcgagaa tttgggccac gtgcttggtg atctggcgtg tctcaacaag ctgccttttg
     7201 atgaagccgg ctttatccaa ctcagacagg ccacctcgtt cagccttagt cagattatcg
     7261 aacttccgtt gtgtgatcag tttggcgttc agcagctgcc gccaataatt tttcattttc
     7321 ttgacaactt cttctgaggg gacgttatca ctcttccctc tatttttatc ggatcttgtc
     7381 aacactttat tatcaataga atcatctttg agaaaagact ggggcacgat ggcatccacg
     7441 tcgtagtcgg agagccgatt gatgtccagt tcctgatcca cgtacatgtc cctgccgttc
     7501 tgcaggtagt acaggtagag cttctcattc tgaagctggg tgttttcaac tgggtgttcc
     7561 ttaaggattt gggaccccag ttcttttata ccctcttcaa tcctcttcat cctttcccta
     7621 ctgttcttct gtcccttctg ggtagtttgg ttctctcggg ccatctcgat aacgatattc
     7681 tcgggcttat gccttcccat tactttgacg agttcatcca cgaccttaac ggtctgcagt
     7741 attccctttt tgatagctgg gctacctgca agattagcga tgtgctcgtg aagactgtcc
     7801 ccctggccag aaacttgtgc tttctggatg tcctccttaa aggtgagaga gtcatcatgg
     7861 atcaactgca tgaagttccg gttggcaaat ccatcggact taagaaaatc caggattgtc
     7921 tttccactct gcttgtctcg gatcccattg atcagttttc ttgacagccg cccccatcct
     7981 gtatatcggc gcctcttgag ctgtttcatg actttgtcgt cgaagagatg agcgtaagtt
     8041 ttcaagcgtt cttcaatcat ctccctatct tcaaacaacg taagggtgag gacaatgtcc
     8101 tcaagaatgt cctcgttctc ctcattgtcc aggaagtcct tgtctttaat gattttcagg
     8161 agatcgtgat acgttcccag ggatgcgttg aagcgatcct ccactccgct gatttcaaca
     8221 gagtcgaaac attcaatctt tttgaaatag tcttctttga gctgtttcac ggtaactttc
     8281 cggttcgtct tgaagaggag gtccacgata gctttcttct gctctccaga caggaatgct
     8341 ggctttctca tcccttctgt gacgtatttg accttggtga gctcgttata aactgtgaag
     8401 tactcgtaca gcagagagtg tttaggaagc accttttcgt taggcagatt tttatcaaag
     8461 ttagtcatcc tttcgatgaa ggactgggca gaggccccct tatccacgac ttcctcgaag
     8521 ttccagggag tgatggtctc ttctgatttg cgagtcatcc acgcgaatct ggaatttccc
     8581 cgggcgaggg ggcctacata gtagggtatc cgaaatgtga ggattttctc aatcttttcc
     8641 ctgttatctt tcaaaaaggg gtagaaatcc tcttgccgcc tgaggatagc gtgcagttcg
     8701 cccaggtgaa tctggtgggg gatgcttcca ttgtcgaaag tgcgctgttt gcgcaacaga
     8761 tcttctctgt taagctttac cagcagctcc tcggtgccgt ccattttttc caagatgggc
     8821 ttaataaatt tgtaaaattc ctcctggctt gctccgccgt caatgtatcc ggcgtagcca
     8881 tttttagact gatcgaagaa aatttccttg tacttctcag gcagttgctg tctgacaagg
     8941 gccttcagca aagtcaagtc ttggtggtgc tcatcatagc gcttgatcat actagcgctc
     9001 agcggagctt tggtgatctc cgtgttcact cgcagaatat cactcagcag aatggcgtct
     9061 gacaggttct ttgccgccaa aaaaaggtct gcgtactggt cgccgatctg ggccagcaga
     9121 ttgtcgagat catcatcgta ggtgtctttg ctcagttgaa gcttggcatc ttcggccagg
     9181 tcgaagttag atttaaagtt gggggtcagc ccgagtgaca gggcgataag attaccaaac
     9241 aggccgttct tcttctcccc agggagctgt gcgatgaggt tttcgagccg ccgggatttg
     9301 gacagcctag cgctcaggat tgctttggcg tcaactccgg atgcgttgat cgggttctct
     9361 tcgaaaagct gattgtaagt ctgaaccagt tggataaaga gtttgtcgac atcgctgttg
     9421 tctgggttca ggtccccctc gatgaggaag tgtccccgaa atttgatcat atgcgccagc
     9481 gcgagataga tcaaccgcaa gtcagcctta tcagtactgt ctacaagctt cttcctcaga
     9541 tgatatatgg ttgggtactt ttcatggtac gccacctcgt ccacgatatt gccaaagatt
     9601 gggtggcgct cgtgcttttt atcctcctcc accaaaaagg actcctccag cctatggaag
     9661 aaagagtcat ccaccttagc catctcatta ctaaagatct cctgcaggta gcagatccga
     9721 ttctttctgc gggtatatct gcgccgtgct gttcttttga gccgcgtggc ttcggcggtt
     9781 tccccggagt cgaacaggag ggcgccaatg aggttcttct ttatgctgtg gcgatcggta
     9841 ttgcccagaa ctttgaattt tttgctcggc accttgtact cgtccgtaat gacggcccag
     9901 ccgacgctgt ttgtgccgat ggcgagccca atggagtact tcttgtccat tccgcggccc
     9961 accttcctct tcttcttggg ggccatcttg tcatcgtcat ccttgtaatc gatgtcatga
    10021 tctttataat caccgtcatg gtctttgtag tccatggtgg ctcacgacac ctgaaatgga
    10081 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa
    10141 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc
    10201 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta
    10261 ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca
    10321 catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc
    10381 cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc
    10441 catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg
    10501 atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt
    10561 ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca
    10621 cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc
    10681 gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa
    10741 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcgcacg
    10801 cggcggcccc agcgcccagg ccaggcctca actcaagcac gaggcgaagg ggctccttaa
    10861 gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa gctcgggatc aagaatcacg
    10921 tactgcagcc aggtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca
    10981 ggcccgcggg aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa
    11041 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
    11101 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
    11161 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
    11221 cactgacggg caccggagcc aattcccact cctttcaaga cctagaaggt ccattagctg
    11281 caaagattcc tctctgttta aaactttatc catctttgca ttaattaacc aaactggatc
    11341 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
    11401 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
    11461 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc agaattggcg
    11521 cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
    11581 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
    11641 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
    11701 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
    11761 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
    11821 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
    11881 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
    11941 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
    12001 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
    12061 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
    12121 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
    12181 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
    12241 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
    12301 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
    12361 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
    12421 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
    12481 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
    12541 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
    12601 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
    12661 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
    12721 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
    12781 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
    12841 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
    12901 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
    12961 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
    13021 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
    13081 gagacccagt acaggcaaaa cgcgctgctt atatagacct cccaccgtac acgcctaccg
    13141 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
    13201 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
    13261 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
    13321 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
    13381 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
    13441 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
    13501 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
    13561 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
    13621 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
    13681 tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc
    13741 agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg
    13801 cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca
    13861 tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga
    13921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    13981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    14041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    14101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    14161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    14221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    14281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    14341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    14401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    14461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    14521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    14581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    14641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    14701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    14761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    14821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    14881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    14941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    15001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    15061 ccgtagaaaa gatcaaagga tcttc
//
