LOCUS       sequence-482207-        8136 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  Expresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega 
            HiBiT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Kassel S, Yuan K, Bunnag N, Neitzel LR, Lu W, Schwarzkopf A, Maines 
            B, Loberg MA, Xu G, Adams A, McCray AD, Cho A, Rockouski M, Orton G,
            Goldsmith L, Aronno MMA, Spencer ZT, Khan OM, Ye F, Williams C, 
            Lebensohn AM, Rohatgi R, Wang X, Weiss VL, Hong CC, Kettenbach AN, 
            Robbins DJ, Ahmed Y, Lee E
  TITLE     The TRIP12 E3 ligase induces SWI/SNF component BRG1-beta-catenin 
            interaction to promote Wnt signaling.
  JOURNAL   Nat Commun. 2025 Jun 5;16(1):5248. doi: 10.1038/s41467-025-60535-5.
  PUBMED    40473626
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-06-5"; volume: "16(1):5248. doi"; pages: " 
            10.1038/s41467-025-60535-5"
COMMENT     Sequence Label: pRP-BRG1-HA-SmBiT
FEATURES             Location/Qualifiers
     source          1..8136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    81..101
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        162..465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        466..669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     620..640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    690..714
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5806..5832
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5920..5944)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5973..6094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5984..6003)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6038..6057
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6210..6227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6381..6969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6461..6480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7140..7931,7932..8000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7763..7782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8001..8105)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcggcgcg ccgcggccgc caactttgta tagaaaagtt gtagttatta atagtaatca
      121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      661 agcagagctg gtttagtgaa ccgtcagatc caagtttgta caaaaaagca ggctgccacc
      721 atgtccactc cagacccacc cctgggcgga actcctcggc caggtccttc cccgggccct
      781 ggcccttccc ctggagccat gctgggccct agcccgggtc cctcgccggg ctccgcccac
      841 agcatgatgg ggcccagccc agggccgccc tcagcaggac accccatccc cacccagggg
      901 cctggagggt accctcagga caacatgcac cagatgcaca agcccatgga gtccatgcat
      961 gagaagggca tgtcggacga cccgcgctac aaccagatga aaggaatggg gatgcggtca
     1021 gggggccatg ctgggatggg gcccccgccc agccccatgg accagcactc ccaaggttac
     1081 ccctcgcccc tgggtggctc tgagcatgcc tctagtccag ttccagccag tggcccgtct
     1141 tcggggcccc agatgtcttc cgggccagga ggtgccccgc tggatggtgc tgacccccag
     1201 gccttggggc agcagaaccg gggcccaacc ccatttaacc agaaccagct gcaccagctc
     1261 agagctcaga tcatggccta caagatgctg gccagggggc agcccctccc cgaccacctg
     1321 cagatggcgg tgcagggcaa gcggccgatg cccgggatgc agcagcagat gccaacgcta
     1381 cctccaccct cggtgtccgc aacaggaccc ggccctggcc ctggccctgg ccccggcccg
     1441 ggtcccggcc cggcacctcc aaattacagc aggcctcatg gtatgggagg gcccaacatg
     1501 cctcccccag gaccctcggg cgtgcccccc gggatgccag gccagcctcc tggagggcct
     1561 cccaagccct ggcctgaagg acccatggcg aatgctgctg cccccacgag cacccctcag
     1621 aagctgattc ccccgcagcc aacgggccgc ccttcccccg cgccccctgc cgtcccaccc
     1681 gccgcctcgc ccgtgatgcc accgcagacc cagtcccccg ggcagccggc ccagcccgcg
     1741 cccatggtgc cactgcacca gaagcagagc cgcatcaccc ccatccagaa gccgcggggc
     1801 ctcgaccctg tggagatcct gcaggagcgc gagtacaggc tgcaggctcg catcgcacac
     1861 cgaattcagg aacttgaaaa ccttcccggg tccctggccg gggatttgcg aaccaaagcg
     1921 accattgagc tcaaggccct caggctgctg aacttccaga ggcagctgcg ccaggaggtg
     1981 gtggtgtgca tgcggaggga cacagcgctg gagacagccc tcaatgctaa ggcctacaag
     2041 cgcagcaagc gccagtccct gcgcgaggcc cgcatcactg agaagctgga gaagcagcag
     2101 aagatcgagc aggagcgcaa gcgccggcag aagcaccagg aatacctcaa tagcattctc
     2161 cagcatgcca aggatttcaa ggaatatcac agatccgtca caggcaaaat ccagaagctg
     2221 accaaggcag tggccacgta ccatgccaac acggagcggg agcagaagaa agagaacgag
     2281 cggatcgaga aggagcgcat gcggaggctc atggctgaag atgaggaggg gtaccgcaag
     2341 ctcatcgacc agaagaagga caagcgcctg gcctacctct tgcagcagac agacgagtac
     2401 gtggctaacc tcacggagct ggtgcggcag cacaaggctg cccaggtcgc caaggagaaa
     2461 aagaagaaaa agaaaaagaa gaaggcagaa aatgcagaag gacagacgcc tgccattggg
     2521 ccggatggcg agcctctgga cgagaccagc cagatgagcg acctcccggt gaaggtgatc
     2581 cacgtggaga gtgggaagat cctcacaggc acagatgccc ccaaagccgg gcagctggag
     2641 gcctggctcg agatgaaccc ggggtatgaa gtagctccga ggtctgatag tgaagaaagt
     2701 ggctcagaag aagaggaaga ggaggaggag gaagagcagc cgcaggcagc acagcctccc
     2761 accctgcccg tggaggagaa gaagaagatt ccagatccag acagcgatga cgtctctgag
     2821 gtggacgcgc ggcacatcat tgagaatgcc aagcaagatg tcgatgatga atatggcgtg
     2881 tcccaggccc ttgcacgtgg cctgcagtcc tactatgccg tggcccatgc tgtcactgag
     2941 agagtggaca agcagtcagc gcttatggtc aatggtgtcc tcaaacagta ccagatcaaa
     3001 ggtttggagt ggctggtgtc cctgtacaac aacaacctga acggcatcct ggccgacgag
     3061 atgggcctgg ggaagaccat ccagaccatc gcgctcatca cgtacctcat ggagcacaaa
     3121 cgcatcaatg ggcccttcct catcatcgtg cctctctcaa cgctgtccaa ctgggcgtac
     3181 gagtttgaca agtgggcccc ctccgtggtg aaggtgtctt acaagggatc cccagcagca
     3241 agacgggcct ttgtccccca gctccggagt gggaagttca acgtcttgct gacgacgtac
     3301 gagtacatca tcaaagacaa gcacatcctc gccaagatcc gttggaagta catgattgtg
     3361 gacgaaggtc accgcatgaa gaaccaccac tgcaagctga cgcaggtgct caacacgcac
     3421 tatgtggcac cccgccgcct gctgctgacg ggcacaccgc tgcagaacaa gcttcccgag
     3481 ctctgggcgc tgctcaactt cctgctgccc accatcttca agagctgcag caccttcgag
     3541 cagtggttta acgcaccctt tgccatgacc ggggaaaagg tggacctgaa tgaggaggaa
     3601 accattctca tcatccggcg tctccacaaa gtgctgcggc ccttcttgct ccgacgactc
     3661 aagaaggaag tcgaggccca gttgcccgaa aaggtggagt acgtcatcaa gtgcgacatg
     3721 tctgcgctgc agcgagtgct ctaccgccac atgcaggcca agggcgtgct gctgactgat
     3781 ggctccgaga aggacaagaa gggcaaaggc ggcaccaaga ccctgatgaa caccatcatg
     3841 cagctgcgga agatctgcaa ccacccctac atgttccagc acatcgagga gtccttttcc
     3901 gagcacttgg ggttcactgg cggcattgtc caagggctgg acctgtaccg agcctcgggt
     3961 aaatttgagc ttcttgatag aattcttccc aaactccgag caaccaacca caaagtgctg
     4021 ctgttctgcc aaatgacctc cctcatgacc atcatggaag attactttgc gtatcgcggc
     4081 tttaaatacc tcaggcttga tggaaccacg aaggcggagg accggggcat gctgctgaaa
     4141 accttcaacg agcccggctc tgagtacttc atcttcctgc tcagcacccg ggctgggggg
     4201 ctcggcctga acctccagtc ggcagacact gtgatcattt ttgacagcga ctggaatcct
     4261 caccaggacc tgcaagcgca ggaccgagcc caccgcatcg ggcagcagaa cgaggtgcgt
     4321 gtgctccgcc tctgcaccgt caacagcgtg gaggagaaga tcctagctgc agccaagtac
     4381 aagctcaacg tggaccagaa ggtgatccag gccggcatgt tcgaccagaa gtcctccagc
     4441 catgagcggc gcgccttcct gcaggccatc ctggagcacg aggagcagga tgagagcaga
     4501 cactgcagca cgggcagcgg cagtgccagc ttcgcccaca ctgcccctcc gccagcgggc
     4561 gtcaaccccg acttggagga gccacctcta aaggaggaag acgaggtgcc cgacgacgag
     4621 accgtcaacc agatgatcgc ccggcacgag gaggagtttg atctgttcat gcgcatggac
     4681 ctggaccgca ggcgcgagga ggcccgcaac cccaagcgga agccgcgcct catggaggag
     4741 gacgagctcc cctcgtggat catcaaggac gacgcggagg tggagcggct gacctgtgag
     4801 gaggaggagg agaagatgtt cggccgtggc tcccgccacc gcaaggaggt ggactacagc
     4861 gactcactga cggagaagca gtggctcaag aaaattacag gaaaagatat ccatgacaca
     4921 gccagcagtg tggcacgtgg gctacaattc cagcgtggcc ttcagttctg cacacgtgcg
     4981 tcaaaggcca tcgaggaggg cacgctggag gagatcgaag aggaggtccg gcagaagaaa
     5041 tcatcacgga agcgcaagcg agacagcgac gccggctcct ccaccccgac caccagcacc
     5101 cgcagccgcg acaaggacga cgagagcaag aagcagaaga agcgcgggcg gccgcctgcc
     5161 gagaaactct cccctaaccc acccaacctc accaagaaga tgaagaagat tgtggatgcc
     5221 gtgatcaagt acaaggacag cagcagtgga cgtcagctca gcgaggtctt catccagctg
     5281 ccctcgcgaa aggagctgcc cgagtactac gagctcatcc gcaagcccgt ggacttcaag
     5341 aagataaagg agcgcattcg caaccacaag taccgcagcc tcaacgacct agagaaggac
     5401 gtcatgctcc tgtgccagaa cgcacagacc ttcaacctgg agggctccct gatctatgaa
     5461 gactccatcg tcttgcagtc ggtcttcacc agcgtgcggc agaaaatcga gaaggaggat
     5521 gacagtgaag gcgaggagag tgaggaggag gaagagggcg aggaggaagg ctccgaatcc
     5581 gaatctcggt ccgtcaaagt gaagatcaag cttggccgga aggagaaggc acaggaccgg
     5641 ctgaagggcg gccggcggcg gccgagccga gggtcccgag ccaagccggt cgtgagtgac
     5701 gatgacagtg aggaggaaca agaggaggac cgctcaggaa gtggcagcga agaagactct
     5761 ggtggcggag gctcgggcgg aggtgggtcg ggtggcggcg gatcataccc atacgatgtt
     5821 ccagattacg cttctggtgg cggaggctcg ggcggaggtg ggtcgggtgg cggcggatca
     5881 atggtgagcg gctggagact gttcaagaag atcagctaaa cccagctttc ttgtacaaag
     5941 tggtgatggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     6001 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     6061 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     6121 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     6181 tggtagcggc cgcggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6541 gcctttctct cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8101 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
