LOCUS       sequence-482294-        9686 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  Dox-inducible expression of Rac1-P29S FLARE sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Weiss BG, Keth JM, Bhatt K, Doyal M, Hahn KM, Noh J, Isogai T, 
            Danuser G
  TITLE     Morphological control of merlin-Rac antagonism in 
            proliferation-promoting signaling.
  JOURNAL   Sci Signal. 2025 May 20;18(887):eadk0922. doi: 
            10.1126/scisignal.adk0922. Epub 2025 May 20.
  PUBMED    40392939
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2025-05-20"; volume: "18(887):eadk0922. doi"; 
            pages: " 10.1126/scisignal.adk0922. Epub 2025 May 20"
COMMENT     Sequence Label: pBabe-Tre-Rac1-P29S (FLARE)
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(1330..1338,1339..1374,1375..1377,1378..1413,
                     1414..1416,1417..1452,1453..1458,1459..2076))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(2077..2130)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2131..2727)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2211..2231)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2708..2727
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2737..3247)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2800..2818)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(3257..3274)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    3292..3316
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3662..4161)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3710..3729)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4103..4126
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    4194..4210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(4224..4802)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFSGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     CDS             complement(join(4830..5540,5541..5543))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKA
                     NFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4872..4893)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(5550..5606)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5616..5669)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6677..6686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6778..6802)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6799..6837)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(6870..6888,6889..6911,6912..6930,6931..6953,6954..6972,
                     6973..6995,6996..7014,7015..7037,7038..7056,7057..7079,
                     7080..7098,7099..7121,7122..7140)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             complement(7181..7597)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7197..7213)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7229..7251)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7798..7997
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             8062..8531
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        8562..8666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8667..8735,8736..9527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8885..8904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg gccatttaat
      841 gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt gaaagacccc
      901 cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt
      961 cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg
     1021 gaggactggc gccccgagtg aggggttgtg ggctcaggga tctcctacct tgatctgaac
     1081 ttctctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     1141 ttgccaaacc tacaggtggg gtctttcatt cccccctttt tctggagact aaataaaatc
     1201 ttttatttta tcgatcacat agcgtaaaag gagcaacata gttaagaata cgatatcttg
     1261 gatcgatcac atagcgtaaa aggagcaaca tagttaagaa tacgatatct tggatcgatc
     1321 cttacttagt tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag
     1381 catatcaagg tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc
     1441 aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat
     1501 tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc
     1561 ttgtctcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac
     1621 ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc
     1681 gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag
     1741 ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg
     1801 tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc
     1861 gccttccagg gggcagaagt gggtatgatg cctgtccagc atctcgattg gcagggcatc
     1921 gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt
     1981 ttgagcgagt ttccttgtcg tcaggccttc gataccgaca ccattgagta attccagagc
     2041 tccgtttatg actttgctct tgtccagtct agacattgga ccagggtttt cttcaacatc
     2101 accacaagtg aggagagaac ctctaccttc ggcaccgggc ttgcgggtca tgcaccaggt
     2161 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     2221 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     2281 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     2341 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     2401 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     2461 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     2521 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     2581 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     2641 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     2701 gcgcaccgtg ggcttgtact cggtcatggt gaattgctgg ggagagaggt cggtgattcg
     2761 gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc ttgcagaatg cggaacaccg
     2821 cgcgggcagg aacagggccc acactaccgc cccacacccc gcctccagca ccgccccttc
     2881 ccggccgctg ctctcggcgc gccctgctga gcagccgcta ttggccacag cccatcgcgg
     2941 tcggcgcgct gccattgctc cctggcgctg tccgtctgcg agggtactag tgagacgtgc
     3001 ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag gaaccttccc gacttagggg
     3061 cggagcagga agcgtcgccg gggggcccac aagggtagcg gcgaagatcc gggtgacgct
     3121 gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg ccgctgcgtt tcccggaacc
     3181 acgcccagag cagccgcgtc cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc
     3241 caaccccgga tccttagtgg tggtggtggt ggtggaccgg ttcattacta aaccactttg
     3301 tacaagaaag ctgggtatta ttcaaggtca tcgtgatgct gagaagtttc gttgataacc
     3361 tgtccatctc tagtttcaac cgtcttaatc agaagtgtcc tttttgagtg ggtatcaacc
     3421 agagggagtg aatccagatt agtttccctc aggttcaggg aggaaaagtt tggaagaggc
     3481 agagaaatcc tgctctcctc gccttccagc agcttcctgt aggtggcaat ctcaatgtca
     3541 agggccatct taacattgag caggtcttgg tattcacgaa ggtgacgagc catttcctcc
     3601 ttcatattct gaatctcatc ctgcagactg gctgtgtata agaagcttat tggctgcagg
     3661 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
     3721 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
     3781 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
     3841 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
     3901 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
     3961 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
     4021 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
     4081 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
     4141 ggaaaagcgc ctcccctacc cggtagaatt gacctgcagg ggccctcgac aaattgttat
     4201 ccgctcacaa ttccacactc gagtcacaac agcaggcatt ttctcttcct cttcttcacg
     4261 ggaggcgggc agaggactgc tcggatcgct tcgtcaaaca ctgtcttgag gcctcgctgt
     4321 gtgagcgccg agcactccag gtattttacg gcaccaatct ccttagccat ggctagaccc
     4381 tgcggatagg tgatgggagt cagcttcttc tccttcagtt tctcgatcgt gtctttatca
     4441 tccctaagat caagtttagt tcccactagg atgatgggag tgttgggaca gtggtgccgc
     4501 acctcaggat accactttgc acggacattt tcaaatgatg caggactcac aagggaaaag
     4561 caaattaaga acacatctgt ttgcggatag gatagggggc gtaatctgtc ataatcttct
     4621 tgtccagctg tatcccataa gcccagattc accggttttc catctaccat aacattggca
     4681 gaataattgt caaagacagt agggatatat tctccagaaa atgcattggt tgtgtaactg
     4741 atcagtaggc aagttttacc tacagctccg tctcccacca ccacacactt gatggcctgc
     4801 atggatccga gctcagatct gagtccggac ttgtacagct cgtccatgcc gagagtgatc
     4861 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     4921 ctcagtgcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     4981 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgcag
     5041 ttgaggccga agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg
     5101 ccgtggatgg cgttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     5161 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     5221 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     5281 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc
     5341 acgccccagg acagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     5401 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     5461 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     5521 aacagctcct cgcccttgct cacgctagca ggtccagggt tctcctccac gtctccagcc
     5581 tgcttcagca ggctgaagtt agtagctccg cttcctgggc caggattctc ctcgacgtca
     5641 ccgcatgtta gcagacttcc tctgccctct ccactgccat taattttctg gctgttggat
     5701 gtcttcttcg agttgtaaaa ctccaacaca tccagaacag cctgcgggtt tttcttctgc
     5761 tccgacttag tgatatttga tgtctgaagc aagcgggccc actgctctgg cattcccgta
     5821 aactcccctg tgacagcatc aaaaccgaca tgaattgtgt gttcaaaatc tgaagggaga
     5881 gaaatctctg gccgctcttt ctctttcttt ttatttgttt tatctccagg taaaatggat
     5941 ccgagctcag atctgagtcc ggacttgtac agctcgtcca tgccctcggt gatgccggcg
     6001 gcggtcagga actccagcag caccatgtgg tcccgcttct cgttggggtc cttgaacagg
     6061 gcgctctggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     6121 gtgttctgct ggtagtggtc ggccagctgc acgccgccgt cctcgatgtt gtgccggatc
     6181 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatgtacac gttgtggctg
     6241 ttgtagttgt actccagctt gtggcccagg atgttgccgt cctccttgaa gtcgatgccc
     6301 ttcagctcga tccggttcac cagggtgtcg ccctcgaact tcacctcggc ccgggtcttg
     6361 tagttgccgt cgtccttgaa gaagatggtc cgctcctgca cgtagccctc gggcatggcg
     6421 ctcttgaaga agtcgtgctg cttcatgtgg tcggggtacc gggcgaagca ctgcacgccg
     6481 tagcccaggg tggtcaccag ggtgggccag ggcacgggca gcttgccggt ggtgcacagc
     6541 agcttcaggg tcagcttgcc gtaggtggcg tcgccctcgc cctcgccgct cacgctgaac
     6601 ttgtggccgt tcacgtcgcc gtccagctcc accaggatgg gcaccacgcc ggtgaacagc
     6661 tcttcgccct tgctcaccat ggtggcgaat tcactttacg gatctggggc cgcggaggct
     6721 ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat
     6781 ctgacggttc actaaacgag ctctgcttat ataggcctcc caccgtacac gcctacctcg
     6841 acccgggtac cgagctcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
     6901 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
     6961 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
     7021 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
     7081 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     7141 gtggtaaact cgacgacgaa agggcctcgt ggatccggcc ggcgcctaga gaaggagtga
     7201 gggctggata aagggaggat cgaggcgggg tcgaacgagg aggttcaagg gggagagacg
     7261 gggcggatgg aggaagagga ggcggaggct tagggtgtac aaagggcttg acccagggag
     7321 gggggtcaaa agccaaggct tcccaggtca cgatgtaggg gacctggtct gggtgtccat
     7381 gcgggccagg tgaaaagacc ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc
     7441 cgtctcgcgg ccatccgacg ttaaaggttg gccattctgc agagcagaag gtaacccaac
     7501 gtctcttctt gacatctacc gactggttgt gagcgatccg ctcgacatct ttccagtgac
     7561 ctaaggtcaa acttaaggga gtggtaacag tctggcccta attttcagac aaatacagaa
     7621 acacagtcag acagagacaa cacagaacga tgctgcagcg ctgcagcaga caagacgcgc
     7681 ggcttcggtt ccaaaccgaa agcaaaaatt cagacggagg cgggaactgt tttaggttct
     7741 cgtctcctac cagaaccaca tatcctgacg gggtcggatt ccacatcgac tcccttcctc
     7801 aggtcgggcc acaaaaacgg cccccaaagt ccctgggacg tctcccaggg ttgcggccgg
     7861 gtgttcagaa ctcgtcagtt ccaccacggg tccgccagat acagagctag ttagctaact
     7921 agtaccgacg caggcgcata aaatcagtca tagacactag acaatcggac agacacagat
     7981 aagttgctgg ccagcttgcc tcccggtggt gggtcggtgg tccctgggca ggggtctccc
     8041 gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat cactcagagg
     8101 agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg
     8161 atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag tgaggggttg
     8221 tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg
     8281 attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat
     8341 ctgatggttc tctagaaact gctgagggct ggaccgcatc tggggaccat ctgttcttgg
     8401 ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt
     8461 tccatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     8521 cccatattca ggctgcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9541 tttactcata tatactttag attgatttgc ggccgcaaaa ggatctaggt gaagatcctt
     9601 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9661 cccgtagaaa agatcaaagg atcttc
//
