LOCUS       sequence-482360-       12334 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Over-express Sc mec1-S6-PreScission Protease Site-FLAG in yeast 
            (integrated).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12334)
  TITLE     O'Donnell lab plasmids 2025
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Sc mec1-S6-P/FLAG-pRS403/GAL-L
FEATURES             Location/Qualifiers
     source          1..12334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    122..143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(158..163,164..181,182..188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    196..212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     201..223
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     220..236
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..236
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     255..275
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        257..275
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(297..362)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(363..386)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        7548..8212
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(7561..7584)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    7879..7996
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(8240..8256)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8241..8257)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8281..8300)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8282..8300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8310..8327)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8310..8326)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8319..8341)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8467..8922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8554..8573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8764..8785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9182..9844)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(9845..10032)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(10179..10198)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10298..10320
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10358..10376)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10444..10548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10549..10617,10618..11409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10767..10786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11580..12168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12069..12088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(12322..12334,1..5)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
       61 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      121 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      181 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      241 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcttacttg
      301 tcatcgtcat ccttgtagtc gatgtcatga tctttataat caccgtcatg gtctttgtag
      361 tcgggcccct ggaacagaac ttccaggttc agcaggcgca gcagccagct caggctatcg
      421 ccccaaaatg gtaaccaacc aatatacatc ttgcttagat tgtcttctga tgttgcttct
      481 tggatcaatg tttctgtttg gccagcaaca ctcaatacca ggccatcctg cggatctata
      541 ccgcggattt tgtttcttaa gacctttagc gctttttgaa ttgagtggtc catgtttcta
      601 tcgtacataa ttgtttcgat cacattcatc aacgctactt cattttttct cattaaagcc
      661 aacgtgactt cactagactt cttaaatgtt ccttctgtcc caattatgcc caacgcatcc
      721 aataaatttg gtgttagtct gaagggaaca atttctggga caggaagcct tttgcctttc
      781 tcaaataaac agtcgaagtc tacatgaaga actttacccg tctgtatatc tagtaatatg
      841 ttttcacagt gcctatcacc tagacctaat atatggccaa ccattgccat gacggcgtaa
      901 gatctggcat acgtattcct ggcgttgaac caattgattg gatcaggaaa gttttctaaa
      961 aaccattggt acaagattgg aggaaattta tctacctgtt ccatgtaaaa ctcgagtttt
     1021 ccatctactg cggtgtgctg ccacctatca tgtagacttt tcaggctata cttaattttc
     1081 agactttcgt actttgtaga aagaatagat cttaaagtta caacattcgg taccatttcc
     1141 aatatcccac agtcttctcg aagagatagt acggagtaaa tatttatgcc caggcttctt
     1201 tttcttgaag ctatgtcctt actcagaaga aaatccattg ttgtggcgaa ctgcatatat
     1261 tggttatctt gtcggacatc ttccttctta cacatgatcc cataaatgtt gccatctgaa
     1321 cctattatgt tcaattgttt tggcttcttt aatgaagaaa acactttata agatgatccg
     1381 aatctaatta tagaaacaac cggcctaaat ggttgatagc ccctcattga gttagactct
     1441 agtggtgaaa tgatgtctaa attttttctt actggaacaa ccattgcaga tggggccacg
     1501 ttcatgtcaa atttgaagtc tttttctaaa gatttgcctg atctacttgt aatgcttttg
     1561 acatcttgca aacagacacg agttaatgct ttcgttaaat ccaatgcact agaaactaga
     1621 tcatgaggat tttgcgaatg ttgtctatac ttttctaaaa tatgcttacc acgaagaact
     1681 ctttttgaag aattggaatt tacaagggct gtgatatacc ataaaatatg agaggggtat
     1741 tcaacagcca aacttagcag tatgtgcatt attatctggg ccgatgattg atgagaatgt
     1801 aataacctag ataacaactg agtcaaaaca aagtaccaaa tataagtggg acaatgctgc
     1861 agcgcttctt caacatgact acatatatct tccgtagcct tactcagcat ttcctttctg
     1921 tttccaggag cttcagaaat tgatgcggcc gcaatatcta gccaaaacgt gataactttg
     1981 ggcaaatttt ctcttacttt agcagtgttc ttttcaaatg ccaataaaaa gtaagatatt
     2041 gccctgtact caaaacgacc attagtaata taaccctctg cttttttgcg ctcaagtagt
     2101 ctactatagt ataagccaat agagtaatat ggtttatccc atttagaatc aatctgaaaa
     2161 atatcctgat attgtttaat aatttgttcg gacgctgaat tgttcgaaag gtctaaccat
     2221 tcagtaaact ttaatagcac ggcggcacga tcgcgagcat taaccgagga attttcttga
     2281 tacttttcat gtatttcttg cactatcttt aaggctctat cattctcacc ttgcttccat
     2341 agtatttcag caaactccaa ctctgcctga ggcaaccgcc tttccaaaca atgcattaat
     2401 gattcggagg ctatatctag cctagcgttg tttctcgcca attgggctaa agtgaagaat
     2461 gtttttccta agtcagcgtc tgcttgttca ttcattttca attggtcaaa tgactttctc
     2521 attgacaata tgtaatgatt tggcacgaag tcagccccaa tacgttccat tcgataatcg
     2581 agtatggtag tgttactttt gtattcaaac ttatctttcg cagaagataa aaaactgagg
     2641 tcatataaac tgtgtaattt catcaataaa ttctgtttct ttagcagcgt cgtctcttta
     2701 gatgacgtta taaaatttgt tccgataagc ctaaaggaat tgtggatgta tttttgagtc
     2761 cttaatggat cctcgtttga gatggcaatt aaagctttcg caatattgta ctgcaaaagt
     2821 acttctctat cgtcaatatt tcttaaactc tctatttgtt ctacccaatt tttcaaagtt
     2881 tgaacattgc cttctagatt tgcagcttca agacctatgc tgtaccattc cttcacatct
     2941 ggagacaaag aaatttttcc gtctgaagaa tggaacgaag agttcgatat tatttgagaa
     3001 tacaattggt ggtcatacat agactttagc atcctggttg tagttttggg gtcatctgaa
     3061 aatttgccga ggacattaaa gcagtcttgt gcgagtttcc agttttcaga atattgcaat
     3121 tcttcaattt tagaaaccaa gtttcctgta gcaaaggttc tcagtacacc atcgagtgag
     3181 tcaatgtctc ctatttcttc gtatgtgatt tgtaaatttt tcagtagttg tccattttgg
     3241 ttcttatcgt gaggattctg tcgatagcac tgttcaaggt atagagcaga cctttcaaaa
     3301 gaatccgtct ctaaggagcg ttgagctagc aaatctgaag gggttgttcg caaaaactca
     3361 tctattctca aaagcatgtt agttgttttc gtatctttaa tgataaaagt gccgtgtagt
     3421 ttactgtaat tttgtttaaa ctcagttgcc cattttttgc aatattcgaa aactctgaag
     3481 atggattcat agcacattct taacgaatcc acttgcaagt tattcattcc ttccagattg
     3541 cacgtgaaaa tgctgtcaaa ttcaataata atcccgttta aaatatcagc gtatggagtt
     3601 cctttttctg ccttgataat aatgtccaga gaaatataag gtagcaaaaa atttgatagt
     3661 gagccatcat cttccctaat caaagaggaa aacacgtgta atggatgatt ttctgttgta
     3721 cctgttttca ataaatccaa tgtaaacctt ttcacccaaa ttttgtatcc ttccttgaag
     3781 ttattagaag gatattttag cgggacatat tctttccatg attgcgcaag atacaaggaa
     3841 gataaaagcg gatagatggt tgtcttggag acagagttaa acttttccca tagtttggct
     3901 tcatttggat ataattcttt atggttcata tcccatgact ctgaacttag cccgcaatat
     3961 tttaatgatt cctgtatgac aagggcaaca aacaattgct tgctgggatt ctcactttgc
     4021 caaaacgcag gaacgaggat atcatttatg acccatatca agaacttaat agtttgaaca
     4081 ctatcattca aatcataaac ttcgttttct gaatatgttg ttcttttaaa ctcatgcttt
     4141 gtaacgtcta aaacaccaat catactgata catttggcgc acttctcgca taggtccttg
     4201 tctaaatttc taaacttatg agaagtgtct aataaagcac ccaaaactaa tgttatatca
     4261 gaagtttgcc caaccttctt tggtgtaaaa tcaatcgatc tttctgtctg ctttctccga
     4321 agatatacct ctatatcgtc taaattttgg tttatgacat acttgttact acttttcaag
     4381 ttattagcaa atatgggtat aaggtccgtg gtacttctta ttttattcac cattcttgca
     4441 aattggccat cacgagctaa aatacctaat tcaggcttgc ctactaaagc caaagtagtg
     4501 taaggtttta atttcaaaat caggtccgat ttctctttga ttaaggtatc cagtatactg
     4561 tacactatat ttcgaagttt tccgttgaac tcagaccact tttgaagtat gaatgcaatt
     4621 aagctatcta taacagtaga gagttcttca tcatttagat ggcgaactaa cagatgccaa
     4681 catctaaagg cctcgtatcg aacttcctta agcccaagtc ctgtttgcaa acaaatacta
     4741 atctgggcta atgcggaaat tattgatttt ttgggggcat atatgataag aaaagaaatg
     4801 ccattgataa cccttaactt ttcgtagtaa gtggtgcggc cttcaacatc atgtatgtca
     4861 ctcgaaaaaa cttggaaaat acctaagata ttatcctgta gtttcttttg gaacgcttgt
     4921 tcctgatcat ccgtccagtt attgatattt ttgtacttcg aaccatgcct tttgtctttt
     4981 tcaaaattgg tgattaaaaa tcgcaaagag cataaaatca tattagcatt ctcgctgtca
     5041 cttgcatctt tagtaacgct gtttttgtag agcttcgtta tttcagctaa agttttataa
     5101 tcgggaaggt atgcagttat atatcctttg tcaaaagttg gagccctatt taaaaaaagg
     5161 gtttccaaga tatccaaaga aaataatccg tgttttacca aagctacggc aaatatttgc
     5221 ctactgtttt ttttcagtaa attaaccttc atttggttaa ttaaacttgt atcgccatca
     5281 cacataatct tagcaatttc acttagcaca tcgctcttat attgaataat tgcataaggg
     5341 atgatatatc tctggaaaat atcgagaatt gtttttgaag aatatcccaa taattctatt
     5401 aaattttgaa agccaacttt tctttccacg aggttttttc ccaactgtct tagtaataca
     5461 ggtaaaatag gcgatagtaa ttgatatggt gttttcttta aaattttggc catattttta
     5521 atttgcaaag tcatcattat tcgtaaacta taatcatcag aattgaaaat atcaatcagt
     5581 ttcaaaagta gtgtatcaaa tacatcatta gaagtcgtca atgttaattg tgtccaagca
     5641 atgactaaat tttctttcac cactggcaat ttttgagatt gtagaaactt tatcagcgtg
     5701 gcagtgtgtt catcttcgga attatgagag tctgatatat tgaataaagg tataattcta
     5761 gtacttaata aacgtaggta tctgttagaa ttcataaagc acttttgtac cagtttaaag
     5821 ctcttattga tttgaccgtt tccatctgta agttttggag gttgaaagtg tgaaaatatc
     5881 ctatgtagag aaaataagat gccacttaac aaagattcgc taaattcatc tatatttggg
     5941 tgagatagaa agtttgaatg aagtatcctg aagatttctg atttacccgc agcctctggt
     6001 ctatttggat ttattcgcac gaaagggttg ccagttttct caatatcgca taaatgacaa
     6061 acataccaat tatccatatc ctttgtggaa tttggcagag tgccggaaaa cttctctgat
     6121 tctagacagg ctagacgtcc gagaattaca gtccatgatg ttagccttgt tatattgttt
     6181 gtcttgaaac aagtttgaat ggatacagat gcttcattta aaattagctt gaaatcgttt
     6241 ccagctcttt gaaaaaatcc aggacttagc tggttcacgc ggtatttgag caaagttcct
     6301 ctttctgtct tgttaaactt ctttagtact agcaacctta gcttttccag ctgcttatat
     6361 cgatcatcaa agggagaagt gacatttttc aagctcgagt ccggctccga ttggtatacc
     6421 cagatgttga acttattatc gtactttaaa gcttcaacag gtagggacct tcttttcaaa
     6481 tcaaaatata caatcgagag agctttatta gtcaactgcg tgaacggaaa atgttcactt
     6541 tgtgaaaaat ctgcccactg gtcaattggg aagtggtcta caaagcattt tgagaaatcg
     6601 ttagaaatgc aaagagaata tagtcgaaga attcctttgg ccatagtggg gtcattatcc
     6661 aagagcccat acttgttatc caaaaaatga tgcatagtag tcaatacaaa ctttaattgt
     6721 tcacaacaaa ctaacgctgg ggcatccagg gctgtggaaa ttgttctatt gcaaacatag
     6781 tccacaacta gagaaatata tttgagttgg atttcggcat attctagtgc tgctagttcg
     6841 caggaatcaa agacgtaaag aacaatcctg attaatcgtt ggaagttaaa gtcaaatttc
     6901 gtgaacccca aagaagaatc taagaatgct atagagttcc caaaagtact atactccgaa
     6961 gtgaaccagt ataaaagaat gtatagaccc ttcaaggtgt cataaaattt gagaagctca
     7021 gtcttgccca acaagacttc agtcaaggcc tgttcgtaaa gattcatggt gttctcaaaa
     7081 tgtgcagtaa gctgaaactt agcaggtttt ccgtaaaatt cgacacatgc tttgcaccag
     7141 ctgcctaatt ttcgcctcat aaaccatatt ctgtggtaat tatcatagtt cagaacggat
     7201 atgttgagga aatcatctat cagcctttgt atctcaaagt tcccgttgga atccttcata
     7261 acaagcaaga aggggttgta ctccaacaat aaggcaaggg cggaaactgt tttcgaaaat
     7321 atagtatcat ttttcatgat attgttcctt ctttgatctt ttaggtttct tatgagggtg
     7381 ttcaggatct ttaaactctt ggcgtactcc tgagaagaag atggatccgt gggcactttt
     7441 ttaatctgca cctttgaatc cacccccgag ttcaggtctt ttattgccaa tatcaattcg
     7501 tcaagatatt tgacgtgtga ttccatatgc tcactagtgg atccggggtt ttttctcctt
     7561 gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat
     7621 aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg
     7681 catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa
     7741 taaggctaaa aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg
     7801 aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat
     7861 tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc
     7921 gaccggtgaa gacgaggacg cacggaggag agtcttcctt cggagggctg tcacccgctc
     7981 ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt actgaaagtt
     8041 ccaaagagaa ggttttttta ggctaagata atggggctct ttacatttcc acaacatata
     8101 agtaagatta gatatggata tgtatatgga tatgtatatg gtggtaatgc catgtaatat
     8161 gattattaaa cttctttgcg tccatccaaa aaaaaagtaa gaatttttga aaattcaagg
     8221 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccaattcg
     8281 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8341 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8401 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8461 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8521 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8581 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8641 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8701 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8761 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8821 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8881 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     8941 ccttacgcat ctgtgcggta tttcacaccg catagatccg tcgagttcaa gagaaaaaaa
     9001 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg
     9061 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat
     9121 aggtatacat atatacacat gtatatatat cgtatgctgc agctttaaat aatcggtgtc
     9181 actacataag aacacctttg gtggagggaa catcgttggt accattgggc gaggtggctt
     9241 ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga tcattcttgc
     9301 ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaagctt tcaagaaaat
     9361 gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca agttcgacaa
     9421 ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca tccaaaggcg
     9481 caaatcctga tccaaacctt tttactccac gcacggcccc tagggcctct ttaaaagctt
     9541 gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt aagtcaccaa
     9601 tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc atatggtcca
     9661 gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt tctgctactg
     9721 cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca ccctttaaag
     9781 agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct ttctgctctg
     9841 tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc gaagaaatca
     9901 cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa gaaaaaaaaa
     9961 gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat
    10021 agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa aggactgtgt
    10081 tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc ctagcgctca
    10141 ccaagctctt aaaacgggaa tttatggtgc actctcagta caatctgctc tgatgccgca
    10201 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
    10261 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    10321 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
    10381 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    10441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    10561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    10621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    10681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    10741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    10801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    10861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    10921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    10981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    11041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    11101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    11161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    11221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    11281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    11341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    11401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    11461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    11521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    11581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11701 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    11881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12001 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    12061 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    12121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    12181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    12241 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    12301 cgcagccgaa cgaccgagcg cagcgagtca gtga
//
