LOCUS       sequence-482431-        8839 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  -end oriented kinesin for optogenetic pulling experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Pasolli M, Meiring JCM, Conboy JP, Koenderink GH, Akhmanova A
  TITLE     Optogenetic and chemical genetic tools for rapid repositioning of 
            vimentin intermediate filaments.
  JOURNAL   J Cell Biol. 2025 Sep 1;224(9):e202504004. doi: 
            10.1083/jcb.202504004. Epub 2025 Jul 8.
  PUBMED    40627079
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-09-1"; volume: "224(9):e202504004. doi"; pages: 
            " 10.1083/jcb.202504004. Epub 2025 Jul 8"
COMMENT     Sequence Label: iLID-GFP-GCN4-ppKin14
FEATURES             Location/Qualifiers
     source          1..8839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     941..963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1035..1472
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             join(1572..1574,1575..2285)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1572..1574,1575..2285)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1614..1635)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1875..1894)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2222..2243
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2295..2319
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2783..2791
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    3996..4130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4033..4052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4087..4106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4144..4160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4144..4160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4157..4179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4168..4184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4192..4198,4199..4216,4217..4222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4237..4258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4375..4392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4546..5134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4626..4645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5305..6096,6097..6165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5928..5947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6166..6270)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6338..6356
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6394..6416)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6516..6535
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6729..6751
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6743..6760
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6744..6760
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8561..8837
                     /label=chicken beta-actin promoter
ORIGIN
        1 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
       61 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      121 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      181 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      241 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      301 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
      361 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
      421 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
      481 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
      541 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
      601 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
      661 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      721 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      781 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      841 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg
      901 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      961 accggcgggg tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg
     1021 ggcgcgccac catgctggca accacactgg aacggatcga gaaaaatttc gtgattactg
     1081 atccgagact gcctgacaac ccaatcattt ttgcgagcga ttccttcctg cagctgacag
     1141 aatattctcg ggaagagatc ctggggcgca attgccgttt tctgcaggga cccgagacag
     1201 accgtgccac tgttcggaaa atcagagatg ctattgacaa ccagactgaa gtgaccgttc
     1261 agctgatcaa ttataccaag agcggcaaga agttctggaa cgtgttccac ctgcagccga
     1321 tgcgcgatta taagggcgac gtccagtact tcattggcgt gcagctggat ggcaccgaac
     1381 gtcttcatgg cgccgctgag cgtgaggcgg tctgcctgat caaaaagaca gcctttcaga
     1441 ttgctgaggc agcgaacgac gaaaattact tttctagagg tggcgggggc agtgggggag
     1501 ggggaagtgg aggcggcggt tctggcgggg gaggctccgg tggcgggggg agcgggggtg
     1561 ggagtaagct tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1621 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1681 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1741 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1801 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1861 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1921 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1981 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2041 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2101 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2161 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2221 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2281 acaagcaccg gtatacaagt ttgtacaaaa aagcaggctc cgctattgaa gacaagttgg
     2341 aagaaatact ttcgaaattg taccacattg aaaatgagtt ggccagaatt aagaaattat
     2401 tgggcgaaat tggtggaggt agtggtggag gtagtgcggc cgccagagat gatttgaggg
     2461 gacgacttgt aactctcgag aagaaattag cagagcgtac ccaagatttg agtcttgcag
     2521 agtctacttt ggcaactcgt gagggtgaat tcgaatccct gcaaagcaat ctaatggagc
     2581 ttaatgagct ccgggagatg aaggaggata tcgacagaaa gaatgagcaa acagcagcta
     2641 tattgaagcg acaagcagac cagataactg aattagaaac tctttatagg gaagaacaag
     2701 tccttcgaaa acgatacttc aacatgatgg aagacatgaa aggtaagatc agggtatatg
     2761 ctcggtggag acccctaagt agtaaggagg tcaaagagcg gcaacaaaac gtgttgatag
     2821 cacctgatga attcaccatc gaacatcctt ggaaagacga caaacccaaa caacatcaat
     2881 ttgatcacgt tttcgaccac catgcgaccc aagaagaggt ctttgaggat acaaagtatt
     2941 tggtccaatc agcaattgac ggatacaatg tctgtatatt tgcatacggg caaactgggt
     3001 caggcaaaac gtttacgatt tacggttctg acaacaaccc aggtctaact ccacgagcta
     3061 ccaaagaact ttttggctat cttaagcgag acgccaataa gttttcgttt gctctgaagg
     3121 tttacatgct tgaaatatat caagattcgc tcatcgatct gcttctacca aagagtgctg
     3181 caaagccacg aaagcttgag attaagaagg actccaaggg aatggttgtc gtagagaatg
     3241 caactctact gccgattgca tcccatgatg aactgcaagc cattgttcat aagggtctgg
     3301 agagacgaca tgtttcgggc acccacatga atgcggagag ttcacggtca cacctcattc
     3361 tatcagtaat cgttgaaagc accaaccgac agtctcaagt tcttgtgaag ggcaagctga
     3421 gttttgtgga tcttgcgggc tctgaaagag tgaagaaatc tggatccagt ggtgaacaac
     3481 tgaaagaggc ccaaagcatt aataaatctt tgtcagcact aggtgatgtc attagcgccc
     3541 ttgcaacaga ggagcagcat attccttacc gtaaccacaa gctaacaatg cttatgagcg
     3601 attctctcgg tggaaatgcg aagaccctca tgtttgtaaa tatctcacca gcagagtcta
     3661 atctggacga gacccataat tcactctgct atgccacaag ggtaagatcc atcatcaatg
     3721 aggccagtaa aaatactaca acgaaagaga tactgcgact gaagaaacaa atcgcgtact
     3781 ggaaggagca agcgggcagg atgggagggt ccgaggatct ggaagaggtt atggatgatc
     3841 gtggaatcaa aactgcagat taaacgcgta ccggtcctag ataagtaatg atcataatca
     3901 gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3961 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4021 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4081 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aattcgtaat
     4141 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6301 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     6361 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6481 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     6541 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6601 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6661 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6721 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagttgg gatctttgca
     6781 ttggcccacg gctctcagga tggggatgct ccccttcagc acccggttcc ccttggaaac
     6841 tgatggtcct ggctctgtgg catggcagtg gcactgtgag gagcccccta ccagcagcac
     6901 acagtggggt tggcactgcc acgctccgga tgccgcgctc tgatccaacc ccataatcaa
     6961 gggaacccga attgccccat cattgccccc accaccccca tcctgccggg ccctcacacc
     7021 ccacgctgcc ttctggtgac attccccagc ccaaacccac ggcttcatgg ctaccgcggg
     7081 gcatttccca ttgccgcccc attatcagct ctgcacacct cccgctgtac ccatgcctcg
     7141 tggctgccct tctttgacgt ataatcttct aattaatacc cggccttgtc aaagtggagc
     7201 acaaacgtta attaattccc cagcaggcag gtaattaaca gtgtgactcc ctttttgctg
     7261 cgagtggggc tgatacagag agatgtggca ctatggagcc cacggggtcc tggcactggg
     7321 tgcccacgga ggtccccatg tgctgcagtg tcaccgcctc cgaggagaca gtattgtccc
     7381 tgcggtgtcc ctgcagctca gctctgtcca cagggccacc tccagtttgg aggggacaca
     7441 atgcagcccc gatgcaaccc atcctcgcag catcccaggg acaaagaccc cactgcaaga
     7501 ccgcacacag ggctgggtcc cgctccccta atatctacag tgcttcttgc atggcccctt
     7561 aatcaatgca gttaatcagc atgcgctcat gcaccgctct ggagctgcaa agcccctcgc
     7621 agcgctgctc accaacaccg cgcaccgccc cggcccagcc tgcagcacgc gctgcaaaca
     7681 ggaaagaaac aaaatattgc ccaaatgtag gcaaaggcat tcggctgcct tgacctccgc
     7741 cgggccgggc cctgcctgac tcagctcctt actcagcgct cgcttcctcc ctccggctgc
     7801 caccgccgca gcgcacaccc tgacaaagag tggcccttaa cgggctctga ggtgcaccca
     7861 gcagtgcact cagcagtcca agggccggcc tggaggtttg caccgctacg tgctgacatt
     7921 agcattgaac ttggccctgg gtagtgctgc aggccgggcg gggtgggtgt agagagtgca
     7981 gcgcgcgttg cacccggtgc cccttcccct cccttgcatc ccagcaggct gcaccccagc
     8041 accaggcccg tgcatgcatg ctcctggtgt tattgcagcc tggtgcatgc atgcgtcttg
     8101 atggtgcagc gctgtgcatg catcctcctt ggtgtgtagc agtctagtgc atgcataccc
     8161 ctcggtgtta ttgctgctct gtgcacgcac gctcattgta tcacttcatc ccagtgcatg
     8221 cactcacact ggagcgattg ctgctcggtg cacgcacact cattgtatca ctgcagctca
     8281 gtggctgcac gcacaccggt gttattgctg ctcggtgcgt gcatgcacat cagtgtcgct
     8341 gcagctcagt gcatgcacgc tcattgccca tcgctatccc tgcctctcct gctggcgctc
     8401 cccgggaggt gacttcaagg ggaccgcagg accacctcgg gggtgggggg agggctgcac
     8461 acgcggaccc cgctccccct ccccaacaaa gcactgtgga atcaaaaagg ggggaggggg
     8521 gatggagggg cgcgtcacac ccccgcccca caccctcacc tcgaggtgag ccccacgttc
     8581 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     8641 taattatttt gtgcagcgat gggggcgggg gggggggggg gcgcgcgcca ggcggggcgg
     8701 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     8761 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     8821 gaagcgcgcg gcgggcggg
//
