LOCUS       sequence-482450-        5468 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  Expresses mouse Aoah protein in the transposon vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5468)
  TITLE     Cancer immunology data engine reveals secreted AOAH as an 
            immunotherapy target
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PKT2/CLP-Aoah
FEATURES             Location/Qualifiers
     source          1..5468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(143..952)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     866..885
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     944..963
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1305..1323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2271..2297
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4158..4279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4169..4188)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4223..4242
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4381..4399
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4709..4726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4880..5468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4960..4979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt tgccagtgtt
      121 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
      181 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
      241 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
      301 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
      361 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
      421 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
      481 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
      541 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
      601 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttt ccggggatcg
      661 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
      721 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
      781 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga
      841 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
      901 ccatgttgga atttaatcgc ggcctcgacg tttcccgttg aatatggctc ataacacccc
      961 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgcagct ggatccagat
     1021 ccctatacag ttgaagtcgg aagtttacat acacttaagt tggagtcatt aaaactcgtt
     1081 tttcaactac tccacaaatt tcttgttaac aaacaatagt tttggcaagt cagttaggac
     1141 atctactttg tgcatgacac aagtcatttt tccaacaatt gtttacagac agattatttc
     1201 acttataatt cactgtatca caattccagt gggtcagaag tttacataca ctaagttgac
     1261 tgtgccttta aacagcttgg aaaattccag aaaatgatgg tctccctata gtgagtcgta
     1321 ttagatcccg ggattactag tttaaaccta ggatgcatac cggtcaagct agcgataatt
     1381 cctgcaggag tcaatgggaa aaacccattg gagccaagta cactgactca atagggactt
     1441 tccattgggt tttgcccagt acataaggtc aatagggggt gagtcaacag gaaagtccca
     1501 ttggagccaa gtacattgag tcaataggga ctttccaatg ggttttgccc agtacataag
     1561 gtcaatggga ggtaagccaa tgggtttttc ccattactga catgtatact gagtcattag
     1621 ggactttcca atgggttttg cccagtacat aaggtcaata ggggtgaatc aacaggaaag
     1681 tcccattgga gccaagtaca ctgagtcaat agggactttc cattgggttt tgcccagtac
     1741 aaaaggtcaa tagggggtga gtcaatgggt ttttcccatt attggcacat acataaggtc
     1801 aataggggtg actagtggag aagagcatgc ttgagggctg agtgcccctc agtgggcaga
     1861 gagcacatgg cccacagtcc ctgagaagtt ggggggaggg gtgggcaatt gaactggtgc
     1921 ctagagaagg tggggcttgg gtaaactggg aaagtgatgt ggtgtactgg ctccaccttt
     1981 ttccccaggg tgggggagaa ccatatataa gtgcagtagt ctctgtgaac attcaagctt
     2041 ctgccttctc cctcctgtga gtttggtaag tcactgactg tctatgcctg ggaaagggtg
     2101 ggcaggagat ggggcagtgc aggaaaagtg gcactatgaa ccctgcagcc ctaggaatgc
     2161 atctagacaa ttgtactaac cttcttctct ttcctctcct gacaggttgg tgtacagtag
     2221 cttccaccat gggctgcgtt tgctcgtcga atcccgagga cgacgctgct tacccatacg
     2281 atgttccaga ttacgcttcg catatgaagt ttccctggaa agtcttcaag accacccttc
     2341 tgcttcttct gctctctcac tcattggcct ctgtgccctc tgaggaccag ccaggtgaca
     2401 gctactccca tggccaaagc tgtttagggt gtgtggtact ggtatctgtg attgagcaac
     2461 tggctgaagt tcacaactct tcagtccagg tggcgatgga gagactctgc agctacctgc
     2521 ctgaaaagct gttcttgaag accgcctgct atttcttggt tcagacattt ggctcagaca
     2581 tcataaaact actcgatgaa gctatgaagg ctgatgtggt gtgctatgct ctggaatttt
     2641 gcaagcgggg tgctgtccaa ccacagtgtc atctctaccc acttccccag gaggcctggg
     2701 agtctgcact ggagaaggcg agacaagtcc tcaggaggtc ctctaccatg aaatatccca
     2761 gaagtggtcg taacatttgt tccctaccat ttttgaccaa gatctgccag aaaattgaat
     2821 tatctataaa gaaggccgtg ccattcaaag acatagattc agacaagcac agtgttttcc
     2881 caacgctgcg gggctaccac tggcgagggc gtgactgtaa tgacagtgac aagacagtgt
     2941 acccgggcag aaggccagac aactgggata tccatcagga ctccaactgt aatggcatct
     3001 ggggtattga tccgaaagat ggaattccat atgagaagaa attctgtgaa ggttcacagc
     3061 ccaggggaat cattttgctg ggagactcag ctggggctca ttttcacatc ccccctgaat
     3121 ggctgacagc ttcacagatg tctgtgaact ccttcctcaa cctgccgtcc gcccttaccg
     3181 atgagctcaa ctggccccaa ctctctggtg taactggatt tctggactcc acgagtggaa
     3241 ttgaagaaaa atccatttac catcgtttga gaaagagaaa ccattgcaat cacagagact
     3301 atcagagtat ctcaaaaaat ggtgcatctt ccagaaatct gaagaatttt attgaaagct
     3361 tgtctaggaa ccaggcatca gaccatccag ccattgtcct ttatgccatg attggaaacg
     3421 atgtttgtaa ttcgaaagct gacacagtac cagaaatgac cactcctgaa caaatgtacg
     3481 ccaacgtcat gcagactctg acacatctaa attctcacct gcccaatggc agccacgtta
     3541 ttttatacgg cttaccagat ggaacttttc tctgggatag cttgcacaac cgataccacc
     3601 ctctaggcca gctaaataaa gatgtgacct atgcacagtt cttctccttt ctaagatgcc
     3661 tgcagctcaa tccctgtaac ggctggatgt cttccaacaa gaccctccgg actctcactt
     3721 cagagagagc cgaacaactc tccaatacac tgaaaaaaat tgcaaccacc gagacgtttg
     3781 caaacttcga tcttttctac gtggattttg ccttccatga aataatagag gactggcaga
     3841 aaagaggtgg acagccatgg cagcttattg aacctgtgga tggattccac cccaatgagg
     3901 tggcttcact tctgcaagca aatcgtgtct gggaaaagat acagcttcaa tggcctcatg
     3961 tcctgggaaa ggagaatcca ttcaactctc agattgaaga ggtgtttgga gaccaaggag
     4021 gacactgagg cgcggtggac tggtttttat ttctggtgca tttgagaaag ccacgctgtc
     4081 ggattcctcg agaccgtacg tgcgcgcgaa tgcatccaga tctaggtacc agggcccagc
     4141 tagctggcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     4201 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4261 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     4321 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggaattatc
     4381 aattaaccct cactaaaggg agaccaagtt aaacaattta aaggcaatgc taccaaatac
     4441 taattgagtg tatgtaaact tctgacccac tgggaatgtg atgaaagaaa taaaagctga
     4501 aatgaatcat tctctctact attattctga tatttcacat tcttaaaata aagtggtgat
     4561 cctaactgac ctaagacagg gaatttttac taggattaaa tgtcaggaat tgtgaaaaag
     4621 tgagtttaaa tgtatttggc taaggtgtat gtaaacttcc gacttcaact gtatagggat
     4681 ctggtaccat ttaaatctgt tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4741 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4801 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4861 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4921 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4981 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5041 cctttctccc ttcgggaacc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5101 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5161 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5221 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5281 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5341 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5401 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5461 gatctcaa
//
