LOCUS       sequence-482469-        9066 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  pLAM12 vector backbone containing Abe1 recombitron with MspRecT and 
            a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium 
            smegmatis mc2 155.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  TITLE     Shipman lab plasmids 2025
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRECMYC-Abe1
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3907..4495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3987..4006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4826..5641)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5549..5568
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5633..5652
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(5802..5848)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taccgaaccg aagacccggc gcgccggcgt ccggcactcc ggacaggtcg ccgccgcatc
       61 gcgctcggag atcgcgcagg tgagatcgaa gggaccgcag tgggaacaac ggaatgtgta
      121 ggtcggcatg gtcacccctt tccattcacc cgaaatatat ccagatgaaa ataaaagtca
      181 agggtgaacg cccgcgccgg gcgcgggtgg ttacgaagtc aaattcgagg tggtgtcgac
      241 gagttcgcgc aactggtcga cgtccagccc gcacgattca aaagccgcac actcggcctc
      301 ggctaccgcg tccaccaggc ggttgcgcag cgtccgcccc cgcggggtca acgccacgag
      361 cacccgccgg cggtcatgca gatcggcagt ccggtagatg atcccttgcg acacaaggcg
      421 atcgacggtt ctggtgagag tcggacccgt gatcagcgtg gcctgcgcga gatcgctcat
      481 gggcacggtg cgccgtccgg ccaactcgtc gagcacgcgc cagtcctcga tcttgagcga
      541 cgagtccgcg agcacgcgtt cgatcgccga ctccgcgagg tgggtcagac gcaccaacgg
      601 cagcaggagg ttggggcggc gctgctgtcc ggcggccatg gtgggtgtct cctcgtgtcg
      661 acggcggggt ctgttcagaa tgaattattt ctgctagaaa gctttgaagc gcagactaca
      721 cgggatcgac ggtgatctgg agtgcaggac ggcgaggttg agttccgcgt cgggctggtg
      781 attccgcttc aagggccagc gggaatcttc gcgccttcgt gcgaggccgt ggccgagctc
      841 gcggccaagg aggtcaacga ccgaggcggc ctgcagggcc gcaaggtcac catcgaggtg
      901 ctcgacggcg gagcgcccgg cgacgacgtc gcccggaccg tcgccgaccg gttgcgaggt
      961 cacggtctcg acgccgtgac cggctggcac atctcggccg tgcgcaaccg catctccccg
     1021 gtggtccgcg accgcatccc gtacgtctac acctcgttgt acgagggcgg tgaacgcaca
     1081 ccgggcgtgt tctgcacagg cgagacaccg cagatccaga tcgcgcccgc gctcgcctgg
     1141 ctgcgcgacc acttcggcat ccggtcctgg tgcctggtcg gcgatgacta catctggccg
     1201 cgccgttccg ccgcggccgc ccgcgcgtac tgccgagatc tggacctcga gctcagacgg
     1261 gagatctacg tcccgtacgg caccgacgat ttccgcgcac ctgtccgcaa ggccatcgcg
     1321 tcgggggcgc aggccgtgtt gatgctgctc gtcggccagg acgccgtgtt gttcaaccgc
     1381 gagttcgcgc gcgccggcgg gcacgaccgc atggcgcggt tcagcccgct gatggaggag
     1441 aacatgctgc tggccagcgg cgccggctcc accgaaaacc tctatgtcgc cgcggcgtac
     1501 ttcagttcac tggccaccgc gggtgccatg gacctgatgg gcagctacgt cgcccgctac
     1561 ggcgccgacg cgccaccgct caacgcgatg gccgaatcct gttacgaggg tctgcttgcg
     1621 ctcgaggcca tcttccagcg ggcccactcc cccgagatac cggacctgat ggcatccgcg
     1681 cacgatgtcg ggttcgacgg gccccggggc ccgatgtgta tgcgcgacag tcaattcgac
     1741 cagcaggtct acatcgccag cgccgacggc tacgacttcg acatcctcga cacgctggcg
     1801 acgctcgacg cttgagaccg cgtcacttct ttatctagat ttaaagatct ggtaccgcgg
     1861 ccgcgccacg gatgccacca caggcactac aacggagttc gccacgtaca tcaccacaac
     1921 caccgattct ggcggtgagc tccccgatat tcagcggaaa tggcttggta tcgaccaaga
     1981 ttcgtagaac cccgtctcgt ctggctggta ttcaaaacgg gcgcaacgaa acacgcaacg
     2041 agacaggcat ggcccaaacc agaaaactag cgtctaccag gacttttacc tgtccgaccc
     2101 gttgcaacgg aaccccccac ggaacccccg cgacacccgc tccccaattg cgttagaaca
     2161 gcggtggatt gtcggcttcg ttgtgggcct tttgagccgc ttcctgttct gccgcacgct
     2221 ctttcctcgc ccgatagccg agtcgcttaa cggtgtccag atgcagcccg aaatgtttgg
     2281 ccgtttgcgg ccaagagtgg ccctcgtcgt cgtgataggc gcggatgcgt tcgcggcgtg
     2341 cagcctgctc ggcgagccac tcgctgcgtt cctgcgccac gagccggacg acgtggcgtt
     2401 cggatagtcc ggtgattcga gcgccttcgg cggcggtcac gcgccgcttt ttgcggacag
     2461 tcggctgccg gttgtagccg tcgctgtagc cgtcgctcat agcaatgcct ccatggctga
     2521 cgcggacttt gcgcgccgcg caactgtgct cgccgccgtg cgcgctgctg cgcccttccg
     2581 cgagatggcc gactggcgcg cactgagtgt ggcctcgtag accacgatcc cgtccgccca
     2641 aatgcgcgac ttggttgtga tccaacgcca aatgctgttg gcgatggcgc ggacctcgct
     2701 gtccggtagc ggtccgggac acacgtcgtt gcacggaaat tcggcgtttc gcgcgtggca
     2761 ctcggcatag atcgcgcggc cgagtccgtc cacgttccgg gtcggcaggt agatccgcat
     2821 gagggcggga cgataggccc acaacctgac ggaatcgaac agtgcgcaat tccgccctag
     2881 cggcgtcgga gccgctttgt acgtggtctg ctgacgccag cgcggcggtg gcatgttcgc
     2941 gccgagctcg gcctcgatgt ggctgagtgt gtagagatct gagtggagcc attccgtttc
     3001 ccaggcgatg tggccggggt ttttggtcat gaggcctgag taactgcggt cgccatcgac
     3061 ggcgcgccga aggccttcgg cgcacgccgc catgtatgcg agcggcttac gccgcgcgta
     3121 ttcggtgcgt ggaacagggg cgttgagtgc ccacactgcg tgtgcgtggc cgttggcgcg
     3181 attgcccacg atcgcgttgg gcagcggatg ggacccccgg gcgctgagcg ctcggagcgc
     3241 tgcgtctgga tggtctacgt ccacgaccag caggtttgcc agcgctgttg ggttcgcctc
     3301 gatgtaccgg cggcctaggg ccgacgcgcg gctttggcgg tagatcccct cgagcagatc
     3361 gtcgcttgcc agcggccagt acggcagcca gagctgctca aattcgtcgg cgacgtggct
     3421 cacgcttggt agtagaccac gattaatcac cggtgtatgg tccgacacga gctccaagtc
     3481 agatatttcg ctgaggggcc accccacaac tgcacactcc cccgctctcc cgtcgagccc
     3541 tggtggtgga acaccagcga cagccgagca cccccaacca cctgtaccaa ccaggaggaa
     3601 cacatgcgtc gtttcgagga cgtttccggg ccgctgagag ccgctgtggc ggccgtacac
     3661 gccgccttag acccgttaga ccccctgccg cctgaatgcg cgggtacgag ccacacagcg
     3721 cccgaactta cggagctggt gggctcacgc gttgcgctcg gtcgttcggc tgcggcgagc
     3781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccctc
     4081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4321 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4501 tcctttgatc ttttctacgg ggtctgacgc tcatggaact agtgaggtct gcctcgtgaa
     4561 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg
     4621 gagccacggt tggtgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc
     4681 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca
     4741 aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt
     4801 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     4861 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     4921 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     4981 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     5041 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaaga ttatgcattt
     5101 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     5161 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     5221 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     5281 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     5341 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     5401 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     5461 cgctaccttt gccatgtttc agaaacaact ctggcgcatg gggcttccca tacaagcgat
     5521 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     5581 catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca
     5641 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     5701 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tccccccccc
     5761 ccgacgtcag gtggctagct gatcaccgcg gccatgatgg cataaaacga aaggcccagt
     5821 ctttcgactg agcctttcgt tttatttgat gcctggcagt cgatcgtacg ctagttgcag
     5881 atcaaccacc gcgtcggaat tcgccggggc gctcatgcgt tgggcccgtc gaacatgtcg
     5941 atgacagcct tcgcctcgtc gaacgtgagg tcggcggcgc gcgtggcttt gacgccggcc
     6001 gaatcggcga ggaacttgaa ccaatcggcg tcgttgtact tttcggcctt ctggatctcg
     6061 ccgaggcgct tcagctgatc gggattggcc atctgcacgt cgggcgccgg cgcggtggcg
     6121 cgctgcgcgc gttgtgcgcg cgacgacttc ggtggcgtat cgacctcgtg cggctcggcg
     6181 atctcgccgt cgatgtagtc ggccgtcggc ttgacggtcg acggcagcgc gagcgcctgc
     6241 gactgtgaca ggtcggtgcc cgggcgatcg tcggcgcgga tcgccgcgtc gagccgcgtc
     6301 gtcttcggtg ccagcttcag cacctgtttg agcgcggtct tccgctccat ccagcgctgc
     6361 gggtccttga tgtcgcccga ggatccgacc ttgccgcgac gcaattccct gatctcgtcg
     6421 gcggtgaaca cgtcccacag cggctcagcg ccggtcacct cgacgatcgc gtagaagtag
     6481 accggattac cccgatcacc cttggccttc acgtgtttca gcgtcggatt gaggcccatg
     6541 gtgtaatgga attcgtcgtt cgcgccgacc cactgcgcgt cgatccggga ggcgcgcggg
     6601 tgctgccaga acagtttgac gatgccctgg tagccgatga tcagctggca ctcgaccacg
     6661 ccgcgccggg tgtcgcggta gggcacgagg tagcactcgc cgttgacacc gggctcgaga
     6721 ccgagcgcgg ccgaggtcag cagcgcaccg gcaaatgact cttgcgtgca gtcgtcgagg
     6781 gactgcttag cgatgccagc agctttcgcg ttgcgcgact tgcggatctc ggtttgcacg
     6841 atccgcgcga tgcggtcggg gtccataccc ttgggcactg cacgcgccag ctgcggcacg
     6901 agcacctgca gcacttgcga gatcgtctcg ggtgccttgg gcgaatcctg cttggtgaca
     6961 gcattttcag ccatgattgg ctttctcctt tctcccgccg ggatcatgca ggtggcgtgg
     7021 gagcctcata ctaagcaaag cctcaagttg ctttcgcatt gcaccgtgga ggcctctggc
     7081 gccggggtga tggtgtccag cggctcacac gggccccgcc gggcgtgacg cacgacggcg
     7141 cacctccacg gtgcccttct ttggtgggtc ttggcagaac gggcatgata cacaccctcg
     7201 cctgaggctc ccacatcacc tgctctagcc tcatatgaac ggtactatgg cccgaagagg
     7261 ttctccacct caatattatc ggccgccacc cgacgatcga cgtcggcggc ataggacaag
     7321 tgcccgttca ccttgctcag atttttggtc agtttaccgt gggcggccaa gtgacggtag
     7381 gcacggagct tgttgcggta gcccttcgtc aacttgggcg tccccgtatg gaccaagagc
     7441 ccatgcactt tcaggcgggc tggggacacg ctgagtttgg tcttcccggg cgcgagttcc
     7501 cacggcgacg catgaaagac acccgccacg tcatcggtca aacccgatgg cacttcggcc
     7561 gaacccgaga acgtcacatc gtcggcataa cgcgtataat tgcaaccata tttttcggcc
     7621 acgcgcagca gttgcttatc caagtcgtcc gcgcagatgt tgctgagcac cggggaggct
     7681 ggggacccct gcgcgaggcc gccgcgaaaa caacacaacg agctgaccag cgcggcttga
     7741 tcgtggttgt agcccaaccg ttggagacac tctttgacgt agatggatgg ggtcgatgga
     7801 aaaaattctt ggatatcaca cgaaaagacc catttcgcgt ttttgtgttt gatcgccgca
     7861 tcaatatagg tccggccggc cacgaagccg tacacgcagt ccgccggacg ccaggcatcg
     7921 gccaagcgga cgctgagcca tttctgaaag attttcaaga cgacccgtgg cgcatgaatc
     7981 tcgcgttggc cgttcccttt ggggatattg aacgtccggt agtaacggtg gggtttgttg
     8041 ataatggccc acacgatgcc cggattcacg ccaaaaatcg ccgccaccac gctgcccgag
     8101 acgacgggtg ggaggttcaa cgccgccaaa cgattcacct cttgcagctc cgtatcggtg
     8161 atctcacctt ccaacgactt aataaaatcc gacagggtcg ggaagacgac atatgggttg
     8221 gagaagaaat atggtgggag acgcatatgt ggactccctt tctcttatcg ggtggtggcc
     8281 gcgagcgcgg ccggtggagg gttggtgact gacggcgctg cgagacggcg cccgatgggg
     8341 tatgcgagaa caactgcggc aaaaccgatc acggcgatga ccgcggcggg gagcgggccg
     8401 gtttcccagt tgcccgacac gatcaggaag gccggcacgg cggcggtgat cacgccttcg
     8461 gccaccgcga cgaacccgac ggcgggcccc agtgcgtcgt gccccaggcc gagcagcagg
     8521 aacagcatga accacagcac tgcccacagc agccagatca ccccgaacgc cgggtcggcc
     8581 tcggcggtga acgcgtgcca cgagtagccg agtgcggcga tcgcgacgaa cagcgagaac
     8641 catccgagac cttctccgtc ccagtcggtc acgttgttga tggccaccca caggtaggtg
     8701 aagccgaaca ggtagagccc ggaggccgcg aagatcacgg ccgcgtctcc gccggactgc
     8761 aggatcagca ccgtaggcgt caccacctgc agtccgccga cgaagaagtt cagcggtgtt
     8821 gcgcctcgtg ggctgatgcg gcccagcagc atcagcccgt cgatgatgag caccgcaccc
     8881 acgtagaaga gcccgacgcc acccatgtcg ctagtacctc ggcagactcg gtagaccggg
     8941 attgcggcgc ggggcccgcg ggcggtccgc gcctgcggga atcgacttca ccaccgtggg
     9001 actttcggcg ctcgcggacg ccgcgtcgaa tgcgcggtga tgtcccgcgg tgaatgtcgt
     9061 cagccc
//
