LOCUS       sequence-482471-        9112 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  pLAM12 vector backbone containing Csp1 recombitron with MspRecT and 
            a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium 
            smegmatis mc2 155.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  TITLE     Shipman lab plasmids 2025
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRECMYC-Csp1
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4343..4389
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4539..4558)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4550..5365
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4623..4642)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5696..6284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6185..6204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgttcacc gccctcgtac aacgaggtgt agacgtacgg gatgcggtcg cggaccaccg
       61 gggagatgcg gttgcgcacg gccgagatgt gccagccggt cacggcgtcg agaccgtgac
      121 ctcgcaaccg gtcggcgacg gtccgggcga cgtcgtcgcc gggcgctccg ccgtcgagca
      181 cctcgatggt gaccttgcgg ccctgcaggc cgcctcggtc gttgacctcc ttggccgcga
      241 gctcggccac ggcctcgcac gaaggcgcga agattcccgc tggcccttga agcggaatca
      301 ccagcccgac gcggaactca acctcgccgt cctgcactcc agatcaccgt cgatcccgtg
      361 tagtctgcgc ttcaaagctt tctagcagaa ataattcatt ctgaacagac cccgccgtcg
      421 acacgaggag acacccacca tggccgccgg acagcagcgc cgccccaacc tcctgctgcc
      481 gttggtgcgt ctgacccacc tcgcggagtc ggcgatcgaa cgcgtgctcg cggactcgtc
      541 gctcaagatc gaggactggc gcgtgctcga cgagttggcc ggacggcgca ccgtgcccat
      601 gagcgatctc gcgcaggcca cgctgatcac gggtccgact ctcaccagaa ccgtcgatcg
      661 ccttgtgtcg caagggatca tctaccggac tgccgatctg catgaccgcc ggcgggtgct
      721 cgtggcgttg accccgcggg ggcggacgct gcgcaaccgc ctggtggacg cggtagccga
      781 ggccgagtgt gcggcttttg aatcgtgcgg gctggacgtc gaccagttgc gcgaactcgt
      841 cgacaccacc tcgaatttga cttcgtaacc acccgcgccc ggcgcgggcg ttcacccttg
      901 acttttattt tcatctggat atatttcggg tgaatggaaa ggggtgacca tgccgaccta
      961 cacattccgt tgttcccact gcggtccctt cgatctcacc tgcgcgatct ccgagcgcga
     1021 tgcggcggcg acctgtccgg agtgccggac gccggcgcgc cgggtcttcg gttcggtagg
     1081 gctgacgaca ttcaccgcgg gacatcaccg cgcattcgac gcggcgtccg cgagcgccga
     1141 aagtcccacg gtggtgaagt cgattcccgc aggcgcggac cgcccgcggg ccccgcgccg
     1201 caatcccggt ctaccgagtc tgccgaggta ctagcgacat gggtggcgtc gggctcttct
     1261 acgtgggtgc ggtgctcatc atcgacgggc tgatgctgct gggccgcatc agcccacgag
     1321 gcgcaacacc gctgaacttc ttcgtcggcg gactgcaggt ggtgacgcct acggtgctga
     1381 tcctgcagtc cggcggagac gcggccgtga tcttcgcggc ctccgggctc tacctgttcg
     1441 gcttcaccta cctgtgggtg gccatcaaca acgtgaccga ctgggacgga gaaggtctcg
     1501 gatggttctc gctgttcgtc gcgatcgccg cactcggcta ctcgtggcac gcgttcaccg
     1561 ccgaggccga cccggcgttc ggggtgatct ggctgctgtg ggcagtgctg tggttcatgc
     1621 tgttcctgct gctcggcctg gggcacgacg cactggggcc cgccgtcggg ttcgtcgcgg
     1681 tggccgaagg cgtgatcacc gccgccgtgc cggccttcct gatcgtgtcg ggcaactggg
     1741 aaaccggccc gctccccgcc gcggtcatcg ccgtgatcgg ttttgccgca gttgttctcg
     1801 cataccccat cgggcgccgt ctcgcagcgc cgtcagtcac caaccctcca ccggccgcgc
     1861 tcgcggccac cacccgataa gagaaaggga gtccacatat gtcgaactcc caactctgga
     1921 agtactgcac caaagaacaa tgccaggact tcatcttgtc cttggacctc gtcccgcatt
     1981 ccgcggggat gtcgcgcgag aagcagctcg cctgtctgta tatgttgagc aaccgcgggg
     2041 cggaaaacta ccacaaggaa attctcacga aaaaggatgg cggtacccgc accctcatgg
     2101 tgccctgttc gctcctcaag gccgtccagc gcaacatctt gcaccacgtc ttggaacagc
     2161 ggccagtgag cgactgtgcc cgtgcctatc ggaagggtac ggacattctg gcgaacgccg
     2221 ggccccatac ggggcgcaaa caggtcttga aattggatat ttcgcacttc ttcgattcga
     2281 ttttgttccc gcgtgtcatt tccgccgcct ttccagacac cttgtttccg ccggcggccg
     2341 cggcgctgtt ggcgaatctg tgttgctgtc gggagcgcct gccacagggt tccccggcga
     2401 gcccagccat tagcaacttg gtcatgcggc cgttcgacga aagcatgcgg cggtggtgtg
     2461 aagcgcggca gattgtctat acgcgctact gcgatgacat gaccttttcc ggtgattttg
     2521 atcccgccgc ggtctaccat aaggtcaagt cctatttgga ggcgatgggc ttttccctca
     2581 acgagaaaaa gaccaaactc ctgaaggatg gggtccgtca gagcgtgacc gggattgtgg
     2641 tgaacgagaa accgcaggtg tcgcgcgatt atcgcaagaa gttgcgccaa gagatttatt
     2701 attgcatgaa gtacggcgtg accgggcatt tggaacgtct ccgggaagag acgggcaatg
     2761 cggtcgagga acaacgttac atttgttcgg tcctggggcg cattcaattt gtgttgcagg
     2821 tgaatccgga agaccgcgag tttgccgagt actgtcgcat ctggaaagag cgtttcgtca
     2881 aataataccg ttcatatgag gctagagcag gtgataagcc ggccggcagc atatccggga
     2941 aaatattttc cggcctgacg gggagtcccc gccgggagtt agaaacagtg ggcatgcaga
     3001 atactgcacc cgtaatccgc tccaaagtac atccgcacac ttccgtgtgc acaccgcctt
     3061 cggctgttgc gccgtcgtgc gtcacgcccg gcggggcccg tgtgagccgc tggacaccat
     3121 caccccggcg ccagagggca gcttacggta tcggtgtatt gtgccgtccg gcttgccacc
     3181 tgcatgatcc cggcgggaga aaggagaaag ccaatcatgg ctgaaaatgc tgtcaccaag
     3241 caggattcgc ccaaggcacc cgagacgatc tcgcaagtgc tgcaggtgct cgtgccgcag
     3301 ctggcgcgtg cagtgcccaa gggtatggac cccgaccgca tcgcgcggat cgtgcaaacc
     3361 gagatccgca agtcgcgcaa cgcgaaagct gctggcatcg ctaagcagtc cctcgacgac
     3421 tgcacgcaag agtcatttgc cggtgcgctg ctgacctcgg ccgcgctcgg tctcgagccc
     3481 ggtgtcaacg gcgagtgcta cctcgtgccc taccgcgaca cccggcgcgg cgtggtcgag
     3541 tgccagctga tcatcggcta ccagggcatc gtcaaactgt tctggcagca cccgcgcgcc
     3601 tcccggatcg acgcgcagtg ggtcggcgcg aacgacgaat tccattacac catgggcctc
     3661 aatccgacgc tgaaacacgt gaaggccaag ggtgatcggg gtaatccggt ctacttctac
     3721 gcgatcgtcg aggtgaccgg cgctgagccg ctgtgggacg tgttcaccgc cgacgagatc
     3781 agggaattgc gtcgcggcaa ggtcggatcc tcgggcgaca tcaaggaccc gcagcgctgg
     3841 atggagcgga agaccgcgct caaacaggtg ctgaagctgg caccgaagac gacgcggctc
     3901 gacgcggcga tccgcgccga cgatcgcccg ggcaccgacc tgtcacagtc gcaggcgctc
     3961 gcgctgccgt cgaccgtcaa gccgacggcc gactacatcg acggcgagat cgccgagccg
     4021 cacgaggtcg atacgccacc gaagtcgtcg cgcgcacaac gcgcgcagcg cgccaccgcg
     4081 ccggcgcccg acgtgcagat ggccaatccc gatcagctga agcgcctcgg cgagatccag
     4141 aaggccgaaa agtacaacga cgccgattgg ttcaagttcc tcgccgattc ggccggcgtc
     4201 aaagccacgc gcgccgccga cctcacgttc gacgaggcga aggctgtcat cgacatgttc
     4261 gacgggccca acgcatgagc gccccggcga attccgacgc ggtggttgat ctgcaactag
     4321 cgtacgatcg actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     4381 tcgttttatg ccatcatggc cgcggtgatc agctagccac ctgacgtcgg ggggggggga
     4441 aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     4501 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     4561 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata
     4621 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     4681 tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     4741 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     4801 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa
     4861 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     4921 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     4981 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     5041 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     5101 tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     5161 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     5221 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     5281 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     5341 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     5401 cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt tgctgagttg
     5461 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     5521 aaaatcacca actggtccac ctacaacaaa gctctcacca accgtggctc cctcactttc
     5581 tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc
     5641 agacctcact agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5701 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5761 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5821 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5881 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5941 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6001 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6061 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa
     6121 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6181 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6241 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6301 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6361 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6421 gccgaacgac cgagcgcaac gcgtgagccc accagctccg taagttcggg cgctgtgtgg
     6481 ctcgtacccg cgcattcagg cggcaggggg tctaacgggt ctaaggcggc gtgtacggcc
     6541 gccacagcgg ctctcagcgg cccggaaacg tcctcgaaac gacgcatgtg ttcctcctgg
     6601 ttggtacagg tggttggggg tgctcggctg tcgctggtgt tccaccacca gggctcgacg
     6661 ggagagcggg ggagtgtgca gttgtggggt ggcccctcag cgaaatatct gacttggagc
     6721 tcgtgtcgga ccatacaccg gtgattaatc gtggtctact accaagcgtg agccacgtcg
     6781 ccgacgaatt tgagcagctc tggctgccgt actggccgct ggcaagcgac gatctgctcg
     6841 aggggatcta ccgccaaagc cgcgcgtcgg ccctaggccg ccggtacatc gaggcgaacc
     6901 caacagcgct ggcaaacctg ctggtcgtgg acgtagacca tccagacgca gcgctccgag
     6961 cgctcagcgc ccgggggtcc catccgctgc ccaacgcgat cgtgggcaat cgcgccaacg
     7021 gccacgcaca cgcagtgtgg gcactcaacg cccctgttcc acgcaccgaa tacgcgcggc
     7081 gtaagccgct cgcatacatg gcggcgtgcg ccgaaggcct tcggcgcgcc gtcgatggcg
     7141 accgcagtta ctcaggcctc atgaccaaaa accccggcca catcgcctgg gaaacggaat
     7201 ggctccactc agatctctac acactcagcc acatcgaggc cgagctcggc gcgaacatgc
     7261 caccgccgcg ctggcgtcag cagaccacgt acaaagcggc tccgacgccg ctagggcgga
     7321 attgcgcact gttcgattcc gtcaggttgt gggcctatcg tcccgccctc atgcggatct
     7381 acctgccgac ccggaacgtg gacggactcg gccgcgcgat ctatgccgag tgccacgcgc
     7441 gaaacgccga atttccgtgc aacgacgtgt gtcccggacc gctaccggac agcgaggtcc
     7501 gcgccatcgc caacagcatt tggcgttgga tcacaaccaa gtcgcgcatt tgggcggacg
     7561 ggatcgtggt ctacgaggcc acactcagtg cgcgccagtc ggccatctcg cggaagggcg
     7621 cagcagcgcg cacggcggcg agcacagttg cgcggcgcgc aaagtccgcg tcagccatgg
     7681 aggcattgct atgagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa
     7741 aaagcggcgc gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt
     7801 cgtccggctc gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga
     7861 acgcatccgc gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt
     7921 cgggctgcat ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc
     7981 agaacaggaa gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg
     8041 caattgggga gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca
     8101 ggtaaaagtc ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt
     8161 ttcgttgcgc ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga
     8221 taccaagcca tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga
     8281 tgtacgtggc gaactccgtt gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc
     8341 agatctttaa atctagataa agaagtgacg cggtctcaag cgtcgagcgt cgccagcgtg
     8401 tcgaggatgt cgaagtcgta gccgtcggcg ctggcgatgt agacctgctg gtcgaattga
     8461 ctgtcgcgca tacacatcgg gccccggggc ccgtcgaacc cgacatcgtg cgcggatgcc
     8521 atcaggtccg gtatctcggg ggagtgggcc cgctggaaga tggcctcgag cgcaagcaga
     8581 ccctcgtaac aggattcggc catcgcgttg agcggtggcg cgtcggcgcc gtagcgggcg
     8641 acgtagctgc ccatcaggtc catggcaccc gcggtggcca gtgaactgaa gtacgccgcg
     8701 gcgacataga ggttttcggt ggagccggcg ccgctggcca gcagcatgtt ctcctccatc
     8761 agcgggctga accgcgccat gcggtcgtgc ccgccggcgc gcgcgaactc gcggttgaac
     8821 aacacggcgt cctggccgac gagcagcatc aacacggcct gcgcccccga cgcgatggcc
     8881 ttgcggacag gtgcgcggaa atcgtcggtg ccgtacggga cgtagatctc ccgtctgagc
     8941 tcgaggtcca gatctcggca gtacgcgcgg gcggccgcgg cggaacggcg cggccagatg
     9001 tagtcatcgc cgaccaggca ccaggaccgg atgccgaagt ggtcgcgcag ccaggcgagc
     9061 gcgggcgcga tctggatctg cggtgtctcg cctgtgcaga acacgcccgg tg
//
