LOCUS       sequence-482719-       10191 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  Constitutive lentiviral expression of V5-mScarlet-FAM98B fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush 
            JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I,
            Bartel DP, Sabatini DM, Chivukula RR
  TITLE     Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing 
            in GGC repeat disorders.
  JOURNAL   Science. 2025 Jul 17;389(6757):eado2403. doi: 
            10.1126/science.ado2403. Epub 2025 Jul 17.
  PUBMED    40674500
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-07-17"; volume: "389(6757):eado2403. doi"; pages: " 
            10.1126/science.ado2403. Epub 2025 Jul 17"
COMMENT     Sequence Label: pLJC5 mScarlet-FAM98B
FEATURES             Location/Qualifiers
     source          1..10191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3056..3065
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3065..3106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3113..3808
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3246..3264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3514..3533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(5076..5102)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     misc_feature    5285..5402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5451..5961
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5880..5898
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5983..6582
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5983..6002)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6479..6499
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6710..6943
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7015..7136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7052..7071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7106..7125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7176..7311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7331..7350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7332..7350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7360..7377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7360..7376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7369..7391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7518..7973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7605..7624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7815..7836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7999..8103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8104..8172,8173..8964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8322..8341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9135..9723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9624..9643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9877..9894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10011..10032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10047..10052,10053..10070,10071..10077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10085..10101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10090..10112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10109..10125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10109..10125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10144..10164
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10146..10164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtgccac
     3061 catgggtaag cctatcccta accctctcct cggtctcgat tctacgggct ctatggtgag
     3121 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     3181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3241 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     3301 ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca tccccgacta
     3361 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3421 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     3481 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     3541 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     3601 gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga ccacctacaa
     3661 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtg gaccgcaagt tggacatcac
     3721 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     3781 caccggcggc atggacgagc tgtacaaggg tggaggtggg tcgacgatga gagggccgga
     3841 gccgggtccc caaccgacga tggagggaga cgtgctggac acactggagg cgctggggta
     3901 taaaggacca ttgttagaag agcaagccct tacaaaggcg gcagagggtg gattatcttc
     3961 acctgaattt tcagagctct gtatttggtt aggctctcaa ataaaatcat tatgcaactt
     4021 ggaagaaagt atcacgtctg ctggaagaga tgatctagag agcttccagc ttgagataag
     4081 tggcttttta aaagaaatgg catgtccata ttctgtactc atatcaggag atattaaaga
     4141 tcgtttaaaa aagaaggagg actgtttgaa acttctatta tttttaagta cagaacttca
     4201 agcttcacag atattacaga acaagaaaca taaaaattct caattagata aaaatagtga
     4261 agtttatcag gaagttcaag ctatgtttga tacacttggt atacccaagt caacaacttc
     4321 tgacattccg catatgctaa accaagtgga atcaaaggtg aaagatattc tctcaaaggt
     4381 ccagaaaaat catgtgggaa aaccactgct gaaaatggat ttaaattcag aacaggcgga
     4441 acaactggaa agaatcaatg atgctctttc ctgtgaatat gagtgtcgcc gacgaatgtt
     4501 aatgaaacga ttagatgtga ctgtacagtc ctttggatgg tctgatagag caaaggtaaa
     4561 aacagatgat atagcaagaa tttatcagcc taagcgttat gctttgtcac ccaagacaac
     4621 gattacaatg gcacatctac ttgctgctcg tgaagatcta tccaagatca ttaggacaag
     4681 tagtggcacc agccgggaga agaccgcatg tgccattaat aaggtgctga tgggaagggt
     4741 gcctgacagg ggaggccggc cgaatgaaat tgaaccacca cctccagaaa tgcccccttg
     4801 gcaaaagaga caagaaggcg gcggtggaag gggtggttgg ggtggtggtg gtggaggtgg
     4861 tggtagagga ggtggtgggg gtgggggtgg gagaggtggc tgggggggtg gaggaggagg
     4921 ttggggaggt ggtgggggag ggggaggagg gtggggggga ggaggaggag gtggtagagg
     4981 aggtttccaa ggcaggggag attatggtgg aagagggggt tatggtggaa gagggggcta
     5041 tggtggaaga ggctatggag atccatatgg aggaggtggt ggtggtggtg gtggtggtgg
     5101 tggaggaggt ggatatagaa gatactaaaa actataaaaa ttagatagca gtgcttgctt
     5161 ctttatagat acattagaaa tagtgttcca aataacatac ttgaatatca tctttgaagt
     5221 aaacaccatg ttagactccc tgggaattct cgacctcgag acaaatggca gtattcatcc
     5281 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     5341 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     5401 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     5461 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     5521 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     5581 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     5641 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     5701 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     5761 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     5821 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     5881 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     5941 atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc
     6001 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6061 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6121 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6181 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6241 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6301 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6361 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6421 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6481 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6541 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     6601 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     6661 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6721 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6781 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6841 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6901 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6961 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7021 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7081 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7141 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7201 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7261 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     7321 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     7381 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7441 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7501 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8341 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8641 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9781 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9841 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9901 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    10141 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta a
//
