LOCUS       sequence-482723-       11641 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Bennett RD, Strehler EE
  TITLE     Calmodulin-like protein enhances myosin-10 translation.
  JOURNAL   Biochem Biophys Res Commun. 2008 May 2;369(2):654-9. doi: 
            10.1016/j.bbrc.2008.02.056. Epub 2008 Feb 22.
  PUBMED    18295593
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2008.02.056"; journalName: "Biochem Biophys Res 
            Commun"; date: "2008-05-2- 2"; volume: "369"; issue: "2"; pages: 
            "654-9"
COMMENT     Sequence Label: pcDNA3.1-6His-MyoX
FEATURES             Location/Qualifiers
     source          1..11641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     766..785
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        766..784
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             835..852
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             856..888
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(892..900,901..915)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      1142..1151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1266..1275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6038..6047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7135..7152)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7141..7365
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7411..7839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7498..7517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7708..7729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7848..7868)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7853..8182
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8033..8168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8095..8114
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8249..9043
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8303..8322)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8913..8932
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9217..9338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9254..9273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9308..9327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9387..9403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9387..9403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9400..9422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9411..9427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9435..9441,9442..9459,9460..9465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9480..9501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9618..9635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9789..10377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9869..9888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10548..11339,11340..11408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11171..11190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11409..11513)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11588..11607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
       61 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      121 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      661 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
      781 tagggagacc caagctggct agcgtttaaa cttaagctta ccatgggggg ttctcatcat
      841 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      901 gacgatgacg ataaggtacc atggataact tcttcaccga gggaacacgg gtctggctga
      961 gagaaaatgg ccagcatttt ccaagtactg taaattcctg tgcagaaggc atcgtcgtct
     1021 tccggacaga ctatggtcag gtattcactt acaagcagag cacaattacc caccagaagg
     1081 tgactgctat gcaccccacg aacgaggagg gcgtggatga catggcgtcc ttgacagagc
     1141 tccatggcgg ctccatcatg tataacttat tccagcggta taagagaaat caaatatata
     1201 cctacatcgg ctccatcctg gcctccgtga acccctacca gcccatcgcc gggctgtacg
     1261 agcctgccac catggagcag tacagccggc gccacctggg cgagctgccc ccgcacatct
     1321 tcgccatcgc caacgagtgc taccgctgcc tgtggaagcg ctacgacaac cagtgcatcc
     1381 tcatcagtgg tgaaagtggg gcaggtaaaa ccgaaagcac taaattgatc ctcaagtttc
     1441 tgtcagtcat cagtcaacag tctttggaat tgtccttaaa ggagaagaca tcctgtgttg
     1501 aacgagctat tcttgaaagc agccccatca tggaagcttt cggcaatgcg aagaccgtgt
     1561 acaacaacaa ctctagtcgc tttgggaagt ttgttcagct gaacatctgt cagaaaggaa
     1621 atattcaggg cgggagaatt gtagattatt tattagaaaa aaaccgagta gtaaggcaaa
     1681 atcccgggga aaggaattat cacatatttt atgcactgct ggcagggctg gaacatgaag
     1741 aaagagaaga attttattta tctacgccag aaaactacca ctacttgaat cagtctggat
     1801 gtgtagaaga caagacaatc agtgaccagg aatcctttag ggaagttatt acggcaatgg
     1861 acgtgatgca gttcagcaag gaggaagttc gggaagtgtc gaggctgctt gctggtatac
     1921 tgcatcttgg gaacatagaa tttatcactg ctggtggggc acaggtttcc ttcaaaacag
     1981 ctttgggcag atctgcggag ttacttgggc tggacccaac acagctcaca gatgctttga
     2041 cccagagatc aatgttcctc aggggagaag agatcctcac gcctctcaat gttcaacagg
     2101 cagtagacag cagggactcc ctggccatgg ctctgtatgc gtgctgcttt gagtgggtaa
     2161 tcaagaagat caacagcagg atcaaaggca atgaggactt caagtctatt ggcatcctcg
     2221 acatctttgg atttgaaaac tttgaggtta atcactttga acagttcaat ataaactatg
     2281 caaacgagaa acttcaggag tacttcaaca agcatatttt ttctttagaa caactagaat
     2341 atagccggga aggattagtg tgggaagata ttgactggat agacaatgga gaatgcctgg
     2401 acttgattga gaagaaactt ggcctcctag cccttatcaa tgaagaaagc cattttcctc
     2461 aagccacaga cagcacctta ttggagaagc tacacagtca gcatgcgaat aaccactttt
     2521 atgtgaagcc cagagttgca gttaacaatt ttggagtgaa gcactatgct ggagaggtgc
     2581 aatatgatgt ccgaggtatc ttggagaaga acagagatac atttcgagat gaccttctca
     2641 atttgctaag agaaagccga tttgacttta tctacgatct ttttgaacat gtttcaagcc
     2701 gcaacaacca ggataccttg aaatgtggaa gcaaacatcg gcggcctaca gtcagctcac
     2761 agttcaagga ctcactgcat tccttaatgg caacgctaag ctcctctaat cctttctttg
     2821 ttcgctgtat caagccaaac atgcagaaga tgccagacca gtttgaccag gcggttgtgc
     2881 tgaaccagct gcggtactca gggatgctgg agactgtgag aatccgcaaa gctgggtatg
     2941 cggtccgaag accctttcgg gacttttaca aaaggtataa agtgctgatg aggaatctgg
     3001 ctctgcctga ggacgtccga gggaagtgca cgagcctgct gcagctctat gatgcctcca
     3061 acagcgagtg gcagctgggg aagaccaagg tctttcttcg agaatccttg gaacagaaac
     3121 tggagaagcg gagggaagag gaagtgagcc acgcggccat ggtgattcgg gcccatgtct
     3181 tgggcttctt agcacgaaaa caatacagaa aggtccttta ttgtgtggtg ataatacaga
     3241 agaattacag agcattcctt ctgaggagga gatttttgca cctgaaaaag gcagccatag
     3301 ttttccagaa gcaactcaga ggtcagattg ctcggagagt ttacagacaa ttgctggcag
     3361 agaaaaggga gcaagaagaa aagaagaaac aggaagagga agaaaagaag aaacgggagg
     3421 aagaagaaag agaaagagag agagagcgaa gagaagccga gctccgcgcc cagcaggaag
     3481 aagaaacgag gaagcagcaa gaactcgaag ccttgcagaa gagccagaag gaagctgaac
     3541 tgacccgtga actggagaaa cagaaggaaa ataagcaggt ggaagagatc ctccgtctgg
     3601 agaaagaaat cgaggacctg cagcgcatga aggagcagca ggagctgtcg ctgaccgagg
     3661 cttccctgca gaagctgcag gagcggcggg accaggagct ccgcaggctg gaggaggaag
     3721 cgtgcagggc ggcccaggag ttcctcgagt ccctcaattt cgacgagatc gacgagtgtg
     3781 tccggaatat cgagcggtcc ctgtcggtgg gaagcgaatt ttccagcgag ctggctgaga
     3841 gcgcatgcga ggagaagccc aacttcaact tcagccagcc ctacccagag gaggaggtcg
     3901 atgagggctt cgaagccgac gacgacgcct tcaaggactc ccccaacccc agcgagcacg
     3961 gccactcaga ccagcgaaca agtggcatcc ggaccagcga tgactcttca gaggaggacc
     4021 catacatgaa cgacacggtg gtgcccacca gccccagtgc ggacagcacg gtgctgctcg
     4081 ccccatcagt gcaggactcc gggagcctac acaactcctc cagcggcgag tccacctact
     4141 gcatgcccca gaacgctggg gacttgccct ccccagacgg cgactacgac tacgaccagg
     4201 atgactatga ggacggtgcc atcacttccg gcagcagcgt gaccttctcc aactcctacg
     4261 gcagccagtg gtcccccgac taccgctgct ctgtggggac ctacaacagc tcgggtgcct
     4321 accggttcag ctctgagggg gcgcagtcct cgtttgaaga tagtgaagag gactttgatt
     4381 ccaggtttga tacagatgat gagctttcat accggcgtga ctctgtgtac agctgtgtca
     4441 ctctgccgta tttccacagc tttctgtaca tgaaaggtgg cctgatgaac tcttggaaac
     4501 gccgctggtg cgtcctcaag gatgaaacct tcttgtggtt ccgctccaag caggaggccc
     4561 tcaagcaagg ctggctccac aaaaaagggg ggggctcctc cacgctgtcc aggagaaatt
     4621 ggaagaagcg ctggtttgtc ctccgccagt ccaagctgat gtactttgaa aacgacagcg
     4681 aggagaagct caagggcacc gtagaagtgc gaacggcaaa agagatcata gataacacca
     4741 ccaaggagaa tgggatcgac atcattatgg ccgataggac tttccacctg attgcagagt
     4801 ccccagaaga tgccagccag tggttcagcg tgctgagtca ggtccacgcg tccacggacc
     4861 aggagatcca ggagatgcat gatgagcagg caaacccaca gaatgctgtg ggcaccttgg
     4921 atgtggggct gattgattct gtgtgtgcct ctgacagccc tgatagaccc aactcgtttg
     4981 tgatcatcac ggccaaccgg gtgctgcact gcaacgccga cacgccggag gagatgcacc
     5041 actggataac cctgctgcag aggtccaaag gggacaccag agtggagggc caggaattca
     5101 tcgtgagagg atggttgcac aaagaggtga agaacagtcc gaagatgtct tcactgaaac
     5161 tgaagaaacg gtggtttgta ctcacccaca attccctgga ttactacaag agttcagaga
     5221 agaacgcgct caaactgggg accctggtcc tcaacagcct ctgctctgtc gtccccccag
     5281 atgagaagat attcaaagag acaggctact ggaacgtcac cgtgtacggg cgcaagcact
     5341 gttaccggct ctacaccaag ctgctcaacg aggccacccg gtggtccagt gccattcaaa
     5401 acgtgactga caccaaggcc ccgatcgaca cccccaccca gcagctgatt caagatatca
     5461 aggagaactg cctgaactcg gatgtggtgg aacagattta caagcggaac ccgatccttc
     5521 gatacaccca tcaccccttg cactccccgc tcctgcccct tccgtatggg gacataaatc
     5581 tcaacttgct caaagacaaa ggctatacca cccttcagga tgaggccatc aagatattca
     5641 attccctgca gcaactggag tccatgtctg acccaattcc aataatccag ggcatcctac
     5701 agacagggca tgacctgcga cctctgcggg acgagctgta ctgccagctt atcaaacaga
     5761 ccaacaaagt gccccacccc ggcagtgtgg gcaacctgta cagctggcag atcctgacat
     5821 gcctgagctg caccttcctg ccgagtcgag ggattctcaa gtatctcaag ttccatctga
     5881 aaaggatacg ggaacagttt ccaggaaccg agatggaaaa atacgctctc ttcacttacg
     5941 aatctcttaa gaaaaccaaa tgccgagagt ttgtgccttc ccgagatgaa atagaagctc
     6001 tgatccacag gcaggaaatg acatccacgg tctattgcca tggcggcggc tcctgcaaga
     6061 tcaccatcaa ctcccacacc actgctgggg aggtggtgga gaagctgatc cgaggcctgg
     6121 ccatggagga cagcaggaac atgtttgctt tgtttgaata caacggccac gtcgacaaag
     6181 ccattgaaag tcgaaccgtc gtagctgatg tcttagccaa gtttgaaaag ctggctgcca
     6241 catccgaggt tggggacctg ccatggaaat tctacttcaa actttactgc ttcctggaca
     6301 cagacaacgt gccaaaagac agtgtggagt ttgcatttat gtttgaacag gcccacgaag
     6361 cggttatcca tggccaccat ccagccccgg aagaaaacct ccaggttctt gctgccctgc
     6421 gactccagta tctgcagggg gattatactc tgcacgctgc catcccacct ctcgaagagg
     6481 tttattccct gcagagactc aaggcccgca tcagccagtc aaccaaaacc ttcacccctt
     6541 gtgaacggct ggagaagagg cggacgagct tcctagaggg gaccctgagg cggagcttcc
     6601 ggacaggatc cgtggtccgg cagaaggtcg aggaggagca gatgctggac atgtggatta
     6661 aggaagaagt ctcctctgct cgagccagta tcattgacaa gtggaggaaa tttcagggaa
     6721 tgaaccagga acaggccatg gccaagtaca tggccttgat caaggagtgg cctggctatg
     6781 gctcgacgct gtttgatgtg gagtgcaagg aaggtggctt ccctcaggaa ctctggttgg
     6841 gtgtcagcgc ggacgccgtc tccgtctaca agcgtggaga gggaagacca ctggaagtct
     6901 tccagtatga acacatcctc tcttttgggg cacccctggc gaatacgtat aagatcgtgg
     6961 tcgatgagag ggagctgctc tttgaaacca gtgaggtggt ggatgtggcc aagctcatga
     7021 aagcctacat cagcatgatc gtgaagaagc gctacagcac gacacgctcc gccagcagcc
     7081 agggcagctc caggtgaagg cggtctagag ggcccgttta aacccgctga tcagcctcga
     7141 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7201 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7261 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7321 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7381 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     7441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7621 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7741 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7801 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     7861 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7921 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7981 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8041 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8101 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     8161 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     8221 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     8281 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     8341 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     8401 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     8461 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     8521 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     8581 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     8641 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     8701 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     8761 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     8821 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     8881 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     8941 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     9001 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     9061 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     9121 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     9181 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     9241 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     9361 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    10021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    10081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    10141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    10201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    10261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    10321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10501 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10561 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10621 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10681 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10741 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10801 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10861 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10921 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10981 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    11041 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    11101 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    11161 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    11221 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    11281 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11341 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11401 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11461 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11521 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    11581 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    11641 t
//
