LOCUS       sequence-482774-        3771 bp    DNA     circular SYN 11-AUG-2025
DEFINITION  Plasmid insert contains a gene sequence for Human serum albumin for 
            use in Combinatorial Golden Gate Assembly (BsaI restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: HSA_3b*_AP
FEATURES             Location/Qualifiers
     source          1..3771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(199..216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(199..215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(208..230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        317..419
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             420..1079
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(486..505)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1100..1194
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1302..1847
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1748..1767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2008..2030
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2027..2043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2027..2043
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aacggacatt gttgtagata ttgcgcaaac gctatcaaaa caagtgcctt gaaattttct
       61 tcacctaaat ccttgaaacg atgtgcaact tctgatttat gggcatccct cctgaataca
      121 ccagggtgag accgatgaga cgatgcaggt caggcggaat ggcacttcct tattccagat
      181 gcgtgcggat tatgcatgac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgcttgtggt ggatcacagt tattggagac ttccttggcc gtacgatggg ttttgtttca
      301 ggtatcaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
      361 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      421 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      481 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      541 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      601 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
      661 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      721 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      781 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      841 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      901 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      961 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1021 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1081 ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
     1141 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     1201 actagtaaca acttatatcg tatgggggga cgatttgaag ccccctaccc atggacggcg
     1261 gctttgttga atagagcgtc agacttaata agatgatctt cttgagatcg ttttggtctg
     1321 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
     1381 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
     1441 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
     1501 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
     1561 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     1621 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     1681 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     1741 cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
     1801 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg
     1861 cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg
     1921 ttcgtaagcc atttccgctc gccgaactga actccaccta atgtcttgga gattgtcgga
     1981 tagcgcggaa taggattgtg ccctctgagc ggataacaat ttcacacagg aaacagctat
     2041 gaccatgaca catgcatcca ccatcgcaga cttatcatca ctgagcctcc acctagccta
     2101 tgccgtctca ggtctcagga tccgaggccc agggcagctt gtgaagcagc taccaatttc
     2161 ttaccttcct cagcgaagca agtttctttg tcgtcggctt tacaacactt ctcgacgaaa
     2221 gcagcaaaat catccatcac tgcctttaac tgttcttttg tggcttttgg tttgtgtttg
     2281 accaattcaa ctaaagcggt ctgctttttg atctgtcttt ctttctccga taaggtgcaa
     2341 atatcggcat ggaaggtaaa agtctcagca ttaaactctt taggcacata tgtctcgtcg
     2401 acttccagtg cagaaaagca tggacgccgg ttcaccaaac tctccgtgca gcattttgta
     2461 actctgtcac tcacaggagt cttttcatgt agtacgcata actgattcaa taccacagac
     2521 aaataatctt ccgcacaggg cattctttta gcttctggat gcttacaaca ctttgacccc
     2581 acttttccca agttacgaga aacttcaact aacgtgggtg tagaaacttg tggaactttc
     2641 ttagtatagc gaactaacaa ggcattttga aatttgtatt cacccagttg ttcaaatagt
     2701 tcacagttct gctttataag gttttgaggt tcctcaacaa gtggcttaaa ttcgtcaaag
     2761 actttggcgt agcattcgtg aggatcggca gctgcacaac atttctccag cgttgtttcg
     2821 tatgtttttg ccaatcgaag caacagtaca acagagtagt cgggatgcct tctagcgtac
     2881 tcgtacaaga acatacccaa aaatacatct tttgcttctg cataattctt acagacgtct
     2941 ttactttcca caaaatcagc ggccaagctc ggaagatccg ctggcatctc atcattttcg
     3001 acctcagcga tgcagtggga tttctctagt aaaggtttct cacaacattc cttcaacttt
     3061 gaacttatag aatcctgatt ctcacaaatg tatttagcaa ggtccgctct gtcatcggca
     3121 cattccagta aatccccgtg gcagcattca gtatgaactt tggttagatc agtaacgagt
     3181 ttcgagactt cggcaaattc cgctttgggg aatctctgcg acaatctcgc gactgcccaa
     3241 gccttaaagg ctctttcgcc gaatttttgt aaagacgcac acttcagtct ttgtttagct
     3301 gaggatgctt tgccttcgtc ccttaattca tccaactttg gtaatagaca ggcagccttg
     3361 tctgcggctt ggcaacattc cgtgaatgct gctttatagc gtttggcaaa aaacaatagt
     3421 tctggagcgt aaaaataagg gtgtcgccta gcaatttcat aaagatattt ttttagaaaa
     3481 gtttcttcat tatcatgaaa tgctgtgcac attacatcca cttcaggacg gaccaatctt
     3541 ggtaaattag ggttatcatc cttatgttgt aaaaaacatt cgtttctctc cggttcttgc
     3601 tttgcgcaac agtcagccat ttctccataa gtctcacgta aagtagcaac tgtacataac
     3661 ttatcaccga aaagggtatg caatgactta tcacagttct ctgcggactc gtctgccaca
     3721 caagtcttag cgaattctgt aacttcattt actaatttta catgatcttc g
//
