LOCUS       sequence-482782-        1708 bp    DNA     circular SYN 11-AUG-2025
DEFINITION  Plasmid insert contains the C-myc C-terminal tag sequence for use in
            Combinatorial Golden Gate Assembly (BsaI restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: C-term_C-myc (4a)
FEATURES             Location/Qualifiers
     source          1..1708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..758)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     673..692
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      940..1528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1429..1448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1652..1681
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(join(1702..1708,1..28))
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
       61 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      121 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      181 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      241 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      301 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
      361 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      421 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
      481 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
      541 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
      601 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
      661 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
      721 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
      781 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      841 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
      901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1621 taaccgtagt cggtctcaat ccgccgcagc tgaacaaaaa ttgatttctg aggaagattt
     1681 ataatggctg agaccagacc aataaaaa
//
