LOCUS       sequence-482861-       10749 bp    DNA     circular SYN 11-AUG-2025
DEFINITION  Multiplexable FUCCI sensor (FUCCIplex). The CMV promoter drives the 
            expression of the fluorescent sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Di Sante M, Pezzotti M, Zimmermann J, Enrico A, Deschamps J, Balmas 
            E, Becca S, Reali A, Bertero A, Jug F, Pasqualini FS
  TITLE     CALIPERS: Cell cycle-aware live imaging for phenotyping experiments 
            and regeneration studies
  JOURNAL   bioRxiv 2024.12.19.629259
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.19.629259"
COMMENT     Sequence Label: pLV[Exp]-Hygro-CMV-FUCCIplex
FEATURES             Location/Qualifiers
     source          1..10749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2572..2581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2578..2580,2581..2583,2584..3294)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2623..2644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2884..2903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3231..3252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3349..3621
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             3628..3681
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3688..4632
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             4663..4992
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     protein_bind    complement(4996..5020)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5038..5626
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5091..5111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5509..5520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5657..6156
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5692..5715)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6089..6108
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6173..7198
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6173..7198
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7270..7503
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7575..7696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7612..7631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7666..7685
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7736..7871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7891..7910)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7892..7910)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7920..7937)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7920..7936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7929..7951)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8078..8533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8165..8184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8375..8396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8559..8663
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8664..8732,8733..9524)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8882..8901)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9695..10283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10184..10203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10437..10454
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10571..10592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10607..10612,10613..10630,10631..10637)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10645..10661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10650..10672
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10669..10685
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10669..10685
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10704..10724
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10706..10724
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2641 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2701 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2761 gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     2821 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2881 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2941 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3001 ctggagtaca actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     3061 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     3121 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3181 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3241 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtacagc
     3301 atgctgccta gcctcggcgg ctaccctagc cattggaggc ccctggaacc ctctcctgcc
     3361 agacctgccc tgagagcccc tgcctccgcc acctccggca gcagaaagag agccagacct
     3421 cctgccgccc ctggcagaga tcaggccagg ccccctgccc gcagacggct ccggctgtcc
     3481 gtggatgagg tgtccagccc tagcaccccc gaggcccccg atatccctgc ctgtcccagc
     3541 cctggccaga aaatcaagaa gagcacacct gccgccggac agcctcctca cctgaccagc
     3601 gcccaggacc aggacaccat cgtcgacgag ggcaggggca gcctgctgac ctgcggcgac
     3661 gtggaggaga accccggccc cagatccatg gtagcaggtc atgcctctgg cagccccgca
     3721 ttcgggaccg cctctcattc gaattgcgaa catgaagaga tccacctcgc cggctcgatc
     3781 cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc atcgcgtcat ccaggccagc
     3841 gccaacgccg cggaatttct gaatctcgga agcgtactcg gcgttccgct cgccgagatc
     3901 gacggcgatc tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg
     3961 gtcgcggtgc gctgccggat cggcaatccc tctacggagt actgcggtct gatgcatcgg
     4021 cctccggaag gcgggctgat catcgaactc gaacgtgccg gcccgtcgat cgatctgtca
     4081 ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg gttcactgcg cgcgctgtgc
     4141 gatgacaccg tgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgt
     4201 ttcgatgagc aaggccacgg cctggtattc tccgagtgcc atgtgcctgg gctcgaatcc
     4261 tatttcggca accgctatcc gtcgtcgact gtcccgcaga tggcgcggca gctgtacgtg
     4321 cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc cggtgccgct ggagccgcgg
     4381 ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg
     4441 ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg ccaccctggc ggtgtcgctg
     4501 gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc attatctgcc gcgcttcatc
     4561 cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa ggatcgcgac gcggatcacc
     4621 gcgcttgaga gcggctaccc cagccactgg cggcctctgg aaatgaaccc cagcatgaag
     4681 cagaagcagg aagagatcaa agagaacatc aagaacagca gcgtgcccag gcggaccctg
     4741 aagatgatcc agcccagcgc cagcggatct ctggtgggcc gggagaatga gctgtctgcc
     4801 ggcctgagca agcggaagca ccggaacgac cacctgacca gcaccacctc tagcccaggc
     4861 gtgatcgtgc ccgagagcag cgagaacaag aacctgggcg gcgtgaccca agaaagcttc
     4921 gacctgatga tcaaagaaaa ccccagcagc cagtactgga aagaggtggc cgagaagcgg
     4981 cggaaggccc tgtaaaccca gctttcttgt acaaagtggt gataatcgaa ttccgataat
     5041 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5101 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5161 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5221 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5281 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5341 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5401 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5461 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5521 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5581 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcccg
     5641 cggttcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     5701 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     5761 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     5821 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5881 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5941 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     6001 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     6061 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     6121 gcgctgttct cctcttcctc atctccgggc ctttcgacct cacgtggcca ccatgaaaaa
     6181 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     6241 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     6301 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     6361 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     6421 tagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     6481 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     6541 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     6601 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     6661 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     6721 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     6781 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     6841 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     6901 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     6961 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     7021 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     7081 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     7141 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataggg
     7201 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7261 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7321 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7501 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7561 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7681 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7741 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7861 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7921 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7981 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8041 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8281 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8341 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8401 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8521 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8581 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8641 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8701 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8761 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8821 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8881 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8941 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9001 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9061 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9121 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9181 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9241 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9301 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9361 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9421 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9481 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9541 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9601 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9661 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9721 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9781 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9841 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9901 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9961 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10021 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10081 gtgagctatg agaaagcgcc acgcttcccg aagagagaaa ggcggacagg tatccggtaa
    10141 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10201 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10261 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10321 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10381 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10441 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10501 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10561 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10621 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10681 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10741 tgcaagctt
//
