LOCUS       sequence-482874-       10060 bp    DNA     circular SYN 11-AUG-2025
DEFINITION  This vector encodes of a synCAM (intb2) and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, 
            McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA
  TITLE     Programming multicellular assembly with synthetic cell adhesion 
            molecules.
  JOURNAL   Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z.
            Epub 2022 Dec 12.
  PUBMED    36509107
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05622-z"; journalName: "Nature"; date: 
            "2023-02"; volume: "614"; issue: "7946"; pages: "144-152"
COMMENT     Sequence Label: pHR-flag-GFP-Intb2
FEATURES             Location/Qualifiers
     source          1..10060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..34
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(72..94)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     194..213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     427..446
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     736..753
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             779..1012
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     1102..1118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1103..1119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1122..1710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1228..1239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1637..1657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2069..2779,2780..2782,2783..2785))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(2792..2815)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2907..3314)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3467..3584
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3869..3913)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4098..4331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4828..4953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5000..5633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5801..5935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5825..5844)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5879..5898
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6360..6380)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6510..6575
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(7709..8038)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7723..7858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8023..8043
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8113..8130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8284..8872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8364..8383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9043..9834,9835..9903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9666..9685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9904..10008)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacac ggaattaatt ctagatgctg
      781 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
      841 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
      901 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
      961 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     1021 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     1081 accgagctcg aattaattcc ctcgaggtcg acggtatcga tgcggggagg cggcccaaag
     1141 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     1201 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     1261 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
     1321 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     1381 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     1441 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     1501 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     1561 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     1621 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     1681 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcaa gcttgcatgc
     1741 ctgcaggtcg actctagagt cgcggcctca gtcagtcacg catgttgcag gtgggagttg
     1801 cggccgcaag tcaaggatcc actgtccacc tgccttgctc cacttgacgc gtttaactct
     1861 ccgcgaattt aggattcatt actgtagtcg tcgcagattt gaaaagcgga ttatcattgt
     1921 tccactggct cttcaatttc tctttttcga acctccgata ctcgcgcaga tcggaaagat
     1981 ggataagagc tttccatata acgaggagaa ggatgccaat gaggacaatc ccagcaaccg
     2041 ttccgccgac tattgcagcg atggatccct tatacagctc atccattccc agggtgatcc
     2101 cagcagccgt cacgaattca agcagaacca tatggtctct tttttcgtta ggatccttgg
     2161 agagggctga ttgcgtggag aggtagtggt tatctggcag caaaaccgga ccgtcgccga
     2221 taggcgtgtt ttgctggtaa tggtcggcca gttgcacaga gccatcctcg atattgtgtc
     2281 ggattttgaa gttcaccttg atgccgttct tctgcttatc agccataata tacacgttgt
     2341 ggctgttgta gttgtactcc agcttgtggc ccaaaatatt gccgtcttct ttgaaatcga
     2401 ttcctttcaa ctcaattcta tttaccaacg tgtccccctc gaacttaact tcagccctgg
     2461 ttttatagtt accgtcgtcc ttgaagaata tggtgcgttc ctgaacatag ccctcaggca
     2521 tcgcggactt gaagaaatcg tgctgtttca tgtggtcggg gtaccgggag aagcactgga
     2581 ctccgtaggt gagggtggtc acgagtgtag gccagggcac tggcagcttt cctgttgtac
     2641 agatgaactt caacgtcagt ttaccgtacg tagcgtcgcc ctctccttcg ccgctcactg
     2701 agaacttgtg tccgttaacg tctccgtcca gctcaaccag gatgggcaca actccggtga
     2761 acaattcttc acccttagaa accatggatc ctttatcatc gtcatcttta taatccgcaa
     2821 aaacaagaca aaaaatgtaa ctgagggcga taattgtctt catggtggcg gcgacgcgtg
     2881 agaattctcg agagctccag atcccccccg ggcgactcag tctgtcggag gactggcgcg
     2941 ccgagtgagg ggttgtgagc tcttttatag agctcgggaa gcagaagcgc gcgaacagaa
     3001 gcgagaagca ggctgattgg ttaattcaaa taaggcgcag ggtcatttca ggtccttggg
     3061 ggagcctgga aacatctgat gggtcttaag aaactgctga gggttgggcc atatctgggg
     3121 accatctgtt cttggcctcg ggccggggcc gaaactgcgg tgaccatctg ttcttggccc
     3181 cgggccgggg ccgaaactgc tcaccgcaga tatcctgttt ggcccaacgt tagctatttt
     3241 catgtacccg cccttgatct gaacttctct attcttggtt tggtattttt ccatgccttg
     3301 caaaatggcg ttactgcagc tagcttgcca aacctacagg tggggtcttt cattcccccc
     3361 tttttctgga gactaaataa aatcttttat tttatcgggg ctgcaggaat tcgatatcaa
     3421 gcttatcgat ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     3481 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     3541 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     3601 ccatttggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     3661 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     3721 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     3781 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     3841 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     3901 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     3961 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     4021 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     4081 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     4141 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     4201 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     4261 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     4321 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     4381 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     4441 aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc
     4501 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     4561 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     4621 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     4681 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     4741 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     4801 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     4861 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     4921 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     4981 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     5041 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     5101 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     5161 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
     5221 ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag
     5281 tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc
     5341 cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc
     5401 atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc
     5461 ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata
     5521 tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta
     5581 gatccacaga tcaaggatat cttgtcttct ttgggagtga attagccctt ccaactacta
     5641 agtttgtagt acatatttaa caaatacaat ttctttaaaa tgaaaataat tcagaggaat
     5701 cacaggttta gagtaaatga aaccacaggt aattggcagt ggtaataggg tatggggtgg
     5761 gaagtttggg atgattttgg ttagcttgag ttatccagtt gatccagaca tgataagata
     5821 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5881 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5941 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     6001 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac
     6061 atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga
     6121 gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta
     6181 tgattataac tgttatgcct acttataaag gttacagaat atttttccat aattttcttg
     6241 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta
     6301 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct
     6361 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca
     6421 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc
     6481 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt
     6541 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt
     6601 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat ccggggccca
     6661 ctcataaatc cagttgccgc cacggtagcc aatcaccgta tcgtataaat catcgtcggt
     6721 acgttcggca tcgctcatca caatacgtgc ctggacgtcg aggatttcgc gtgggtcaat
     6781 gccgcgccag atccacatca gacggttaat catgcgatac cagtgaggga tggttttacc
     6841 atcaagggcc gactgcacag gcggttgtgc gccgtgatta aagcggcgga ctagcgtcga
     6901 ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat
     6961 ggatgcagcc tccagaatac ttactggaaa ctattgtaac ccgcctgaag ttaaaaagaa
     7021 caacgcccgg cagtgccagg cgttgaaaag attagcgacc ggagattggc gggacgaata
     7081 cgacgcccat atcccacggc tgttcaatcc aggtatcttg cgggatatca acaacatagt
     7141 catcaaccag cggacgacca gccggttttg cgaagatggt gacaaagtgc gcttttggat
     7201 acatttcacg aatcgcaacc gcagtaccac cggtatccac caggtcatca ataacgatga
     7261 agccttcgcc atcgccttct gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat
     7321 cgtagctgga aatacaaacg gtatcgacat gacgaatacc cagttcacgc gccagtaacg
     7381 cacccggtac agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat
     7441 cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt
     7501 ttcgctcatg tgaagtgtcc cagcctgttt atctacggct taaaaagtgt tcgaggggaa
     7561 aataggttgc gcgagattat agagatctgg cgcactaaaa accagtattt cacatgagtc
     7621 cgcgtctttt tacgcactgc ctctccctga cgcgggataa agtggtattc tcaaacatat
     7681 ctcgcaagcc tgtcttgtgt ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc
     7741 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     7801 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     7861 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     7921 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     7981 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     8041 tccacagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8101 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8701 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10021 aaaagtgcca cctgacgtct aagaaaccat tattatcatg
//
