LOCUS       sequence-483024-        9502 bp    DNA     circular SYN 12-AUG-2025
DEFINITION  Expresses Fc-fusion pST3Gal1 WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Hombu R, Beatty LE, Tomaszewski J, Park S, Neelamegham S
  TITLE     Engineering glycosyltransferases into glycan binding proteins using 
            a mammalian surface display platform.
  JOURNAL   Nat Commun. 2025 Jul 18;16(1):6637. doi: 10.1038/s41467-025-62018-z.
  PUBMED    40681527
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-18"; volume: "16(1):6637. doi"; pages: " 
            10.1038/s41467-025-62018-z"
COMMENT     Sequence Label: pCSCG-Fc-pST3Gal1 WT
FEATURES             Location/Qualifiers
     source          1..9502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2463..2766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2767..2970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3080..3097
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3167..3181
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             4753..4773
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4774..4791
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             5304..5484
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5510..5527)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5516..5740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5786..6214
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5873..5892)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6083..6104
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6223..6243)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6228..6557
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6408..6543
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6470..6489
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6605..6652
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6671..7045
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7175..7296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7212..7231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7266..7285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7345..7361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7345..7361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7358..7380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7369..7385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7393..7399,7400..7417,7418..7423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7438..7459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7576..7593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7747..8335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7827..7846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8506..9297,9298..9366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9129..9148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9367..9471)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2461 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2521 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2581 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2641 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2701 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2761 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2821 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2881 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2941 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     3001 cgccaccatg attcctgcca gatttgccgg ggtgctgctt gctctggccc tcattttgcc
     3061 agggaccctt tgtaccggtc accatcatca tcatcatcag gccaccgaat atgagtacct
     3121 agattatgat ttcctgccag aaacggagcc tccacgacct atgatggatg acgatgacaa
     3181 atctagaaca tgcccaccgt gcccagcacc tgaactcgcg ggggcaccgt cagtcttcct
     3241 cttcccccca aaacccaagg acaccctcat gatctcccgg acccctgagg tcacatgcgt
     3301 ggtggtggac gtgagccacg aagaccctga ggtcaagttc aactggtacg tggacggcgt
     3361 ggaggtgcat aatgccaaga caaagccgcg ggaggagcag tacaacagca cgtaccgtgt
     3421 ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa
     3481 ggtctccaac aaagccctcc cagcccccat cgagaaaacc atctccaaag ccaaagggca
     3541 gccccgagaa ccacaggtgt acaccctgcc cccatcccgg gatgagctga ccaagaacca
     3601 ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc gacatcgccg tggagtggga
     3661 gagcaatggg cagccggaga acaactacaa gaccacgcct cccgtgctgg actccgacgg
     3721 ctccttcttc ctctacagca agctcaccgt ggacaagagc aggtggcagc aggggaacgt
     3781 cttctcatgc tccgtgatgc atgaggctct gcacaaccac tacacgcaga agagcctctc
     3841 cctgtctccg ggtaaaggta gcggctctgg tagcggttcc ggctctcgcc cgtgcacctg
     3901 tacccgctgc atcgaggagc agcgcgttag cgcgtggttt gatgaacgtt tcaatcgcag
     3961 catgcagccg ctgttaaccg cgaaaaatgc gcatctggaa gaagatacct ataaatggtg
     4021 gttacgcctg cagcgtgaga aacagccaaa caacctgaat gatacgatcc gcgagctctt
     4081 ccaagtggtg ccgggcaacg ttgatccgct gctggaaaaa cgcttagtgt cttgtcgccg
     4141 ctgcgccgtg gtgggcaact ctggtaacct gaaagaaagc tactatggcc cgcagattga
     4201 cagccatgac ttcgttctgc gtatgaacaa agcgccgacc gaaggttttg aggcggatgt
     4261 tggtagcaaa accacgcatc attttgttta tccggaaagc ttccgtgaat tagcgcaaga
     4321 agtgagcatg attctggtac cgtttaaaac gaccgatctg gaatgggtga tttctgccac
     4381 gaccacgggc cgtatttctc atacgtatgt tccggtgccg gcgaaaatca aagtgaagaa
     4441 agaaaaaatt ctgatctatc acccggcgtt tattaaatat gtgtttgatc gctggctgca
     4501 gggccacggt cgttatccgt ctacgggcat cctgagcgtg atttttagcc tgcatatttg
     4561 tgacgaggtc gacctgtacg gttttggcgc cgattctaaa ggtaactggc accattattg
     4621 ggaaaacaat ccatctgcgg gtgcgtttcg caagaccggc gttcatgatg gtgatttcga
     4681 gtctaatgtt accaccatcc tggcgagcat taacaaaatt cgtattttca aaggtcgttg
     4741 acctgcaggt tcgaaaacct gtacttccag ggccatcacc atcaccatca ctgactcgag
     4801 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4861 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     4921 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4981 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     5041 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     5101 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     5161 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     5221 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     5281 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5461 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     5521 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5581 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5641 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5701 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5761 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5821 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5881 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5941 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6001 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6061 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6121 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6181 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     6241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6301 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6361 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6421 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6481 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6541 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     6601 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     6661 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     6721 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     6781 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     6841 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     6901 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     6961 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     7021 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     7081 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7141 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7321 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7441 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7501 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9481 ccgaaaagtg ccacctgacg tc
//
