LOCUS       sequence-483025-        9418 bp    DNA     circular SYN 12-AUG-2025
DEFINITION  Displays Fc-fusion pST3Gal1 WT on mammalian cell surface via 
            transmembrane domain of DPP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Hombu R, Neelamegham S
  TITLE     Mammalian Cell Surface Display for High-Throughput Protein 
            Engineering of Glycosyltransferases.
  JOURNAL   Small. 2025 May 26:e2502318. doi: 10.1002/smll.202502318.
  PUBMED    40417887
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small. 2025
            May 26:e2502318. doi: 10.1002/smll.202502318."
COMMENT     Sequence Label: pCSCG-TM(DPP4)-Fc-pST3Gal1 WT
FEATURES             Location/Qualifiers
     source          1..9418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2463..2766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2767..2970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4669..4689
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4690..4707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             5220..5400
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5426..5443)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5432..5656
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5702..6130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5789..5808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5999..6020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6139..6159)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6144..6473
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6324..6459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6386..6405
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6521..6568
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6587..6961
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7091..7212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7128..7147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7182..7201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7261..7277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7261..7277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7274..7296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7285..7301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7309..7315,7316..7333,7334..7339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7354..7375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7492..7509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7663..8251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7743..7762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8422..9213,9214..9282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9045..9064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9283..9387)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2461 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2521 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2581 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2641 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2701 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2761 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2821 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2881 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2941 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     3001 cgccaccatg aagacaccgt ggaagaccgg tgttcttctg ggactgctgg gtgctgctgc
     3061 gcttgtcacc atcatcaccg tgcccgtggt tctgctgtct agaacatgcc caccgtgccc
     3121 agcacctgaa ctcgcggggg caccgtcagt cttcctcttc cccccaaaac ccaaggacac
     3181 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga
     3241 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa
     3301 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca
     3361 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc
     3421 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac
     3481 cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa
     3541 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa
     3601 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct
     3661 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga
     3721 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta aaggtagcgg
     3781 ctctggtagc ggttccggct ctcgcccgtg cacctgtacc cgctgcatcg aggagcagcg
     3841 cgttagcgcg tggtttgatg aacgtttcaa tcgcagcatg cagccgctgt taaccgcgaa
     3901 aaatgcgcat ctggaagaag atacctataa atggtggtta cgcctgcagc gtgagaaaca
     3961 gccaaacaac ctgaatgata cgatccgcga gctcttccaa gtggtgccgg gcaacgttga
     4021 tccgctgctg gaaaaacgct tagtgtcttg tcgccgctgc gccgtggtgg gcaactctgg
     4081 taacctgaaa gaaagctact atggcccgca gattgacagc catgacttcg ttctgcgtat
     4141 gaacaaagcg ccgaccgaag gttttgaggc ggatgttggt agcaaaacca cgcatcattt
     4201 tgtttatccg gaaagcttcc gtgaattagc gcaagaagtg agcatgattc tggtaccgtt
     4261 taaaacgacc gatctggaat gggtgatttc tgccacgacc acgggccgta tttctcatac
     4321 gtatgttccg gtgccggcga aaatcaaagt gaagaaagaa aaaattctga tctatcaccc
     4381 ggcgtttatt aaatatgtgt ttgatcgctg gctgcagggc cacggtcgtt atccgtctac
     4441 gggcatcctg agcgtgattt ttagcctgca tatttgtgac gaggtcgacc tgtacggttt
     4501 tggcgccgat tctaaaggta actggcacca ttattgggaa aacaatccat ctgcgggtgc
     4561 gtttcgcaag accggcgttc atgatggtga tttcgagtct aatgttacca ccatcctggc
     4621 gagcattaac aaaattcgta ttttcaaagg tcgttgacct gcaggttcga aaacctgtac
     4681 ttccagggcc atcaccatca ccatcactga ctcgagacct agaaaaacat ggagcaatca
     4741 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     4801 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     4861 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4921 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     4981 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     5041 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     5101 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     5161 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     5221 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5281 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5341 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5401 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5461 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5521 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5581 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5641 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5701 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5761 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5821 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5881 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5941 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6001 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6061 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6121 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6181 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6241 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6301 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6361 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6421 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6481 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     6541 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     6601 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     6661 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     6721 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     6781 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     6841 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     6901 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     6961 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7021 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7081 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7141 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7201 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7441 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
