LOCUS       sequence-483026-        9439 bp    DNA     circular SYN 12-AUG-2025
DEFINITION  Displays Fc-fusion hST3Gal4 WT on mammalian cell surface via 
            transmembrane domain of DPP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Hombu R, Neelamegham S
  TITLE     Mammalian Cell Surface Display for High-Throughput Protein 
            Engineering of Glycosyltransferases.
  JOURNAL   Small. 2025 May 26:e2502318. doi: 10.1002/smll.202502318.
  PUBMED    40417887
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Small. 2025
            May 26:e2502318. doi: 10.1002/smll.202502318."
COMMENT     Sequence Label: pCSCG-TM(DPP4)-Fc-hST3Gal4 WT
FEATURES             Location/Qualifiers
     source          1..9439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2463..2766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2767..2970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4690..4710
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4711..4728
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             5241..5421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5447..5464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5453..5677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5723..6151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5810..5829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6020..6041
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6160..6180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6165..6494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6345..6480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6407..6426
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6542..6589
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6608..6982
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7112..7233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7149..7168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7203..7222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7282..7298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7282..7298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7295..7317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7306..7322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7330..7336,7337..7354,7355..7360))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7375..7396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7513..7530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7684..8272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7764..7783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8443..9234,9235..9303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9066..9085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9304..9408)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2461 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2521 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2581 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2641 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2701 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2761 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2821 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2881 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2941 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     3001 cgccaccatg aagacaccgt ggaagaccgg tgttcttctg ggactgctgg gtgctgctgc
     3061 gcttgtcacc atcatcaccg tgcccgtggt tctgctgtct agaacatgcc caccgtgccc
     3121 agcacctgaa ctcgcggggg caccgtcagt cttcctcttc cccccaaaac ccaaggacac
     3181 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga
     3241 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa
     3301 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca
     3361 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc
     3421 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac
     3481 cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa
     3541 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa
     3601 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct
     3661 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga
     3721 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta aaggtagcgg
     3781 ctctggtagc ggttccggct ctaaggagcc gtgcctccag ggtgaggcag agagcaaggc
     3841 ctctaagctc tttggcaact actcccggga tcagcccatc ttcctgcggc ttgaggatta
     3901 tttctgggtc aagacgccat ctgcttacga gctgccctat gggaccaagg ggagtgagga
     3961 tctgctcctc cgggtgctag ccatcaccag ctcctccatc cccaagaaca tccagagcct
     4021 caggtgccgc cgctgtgtgg tcgtggggaa cgggcaccgg ctgcggaaca gctcactggg
     4081 agatgccatc aacaagtacg atgtggtcat cagattgaac aatgccccag tggctggcta
     4141 tgagggtgac gtgggctcca agaccaccat gcgtctcttc taccctgaat ctgcccactt
     4201 cgaccccaaa gtagaaaaca acccagacac actcctcgtc ctggtagctt tcaaggcaat
     4261 ggacttccac tggattgaga ccatcctgag tgataagaag cgggtgcgaa agggtttctg
     4321 gaaacagcct cccctcatct gggatgtcaa tcctaaacag attcggattc tcaacccctt
     4381 cttcatggag attgcagctg acaaactgct gagcctgcca atgcaacagc cacggaagat
     4441 taagcagaag cccaccacgg gcctgttggc catcacgctg gccctccacc tctgtgactt
     4501 ggtgcacatt gccggctttg gctacccaga cgcctacaac aagaagcaga ccattcacta
     4561 ctatgagcag atcacgctca agtccatggc ggggtcaggc cataatgtct cccaagaggc
     4621 cctggccatt aagcggatgc tggagatggg agctatcaag aacctcacgt ccttctgacc
     4681 tgcaggttcg aaaacctgta cttccagggc catcaccatc accatcactg actcgagacc
     4741 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4801 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4861 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4921 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     4981 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     5041 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     5101 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5161 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     5221 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5281 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5341 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5401 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     5461 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5521 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5581 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5641 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5701 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5761 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5821 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5881 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5941 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6001 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6061 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6121 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6181 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6241 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6301 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6361 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6421 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6481 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     6541 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     6601 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     6661 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     6721 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     6781 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     6841 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     6901 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     6961 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7021 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7081 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7141 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7201 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7261 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7321 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7381 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7441 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7501 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7561 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7621 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7681 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7801 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7861 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7921 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7981 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8041 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8101 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8161 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8281 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8341 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8401 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8461 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8521 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8581 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8641 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8701 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8761 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8821 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8881 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8941 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9001 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9061 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9121 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9181 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9241 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9301 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9361 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9421 aaaagtgcca cctgacgtc
//
