LOCUS       sequence-483062-       10802 bp    DNA     circular SYN 13-AUG-2025
DEFINITION  Lentiviral SpCas9-gRNA (ZNF865) expression vector with 
            DsRed-Express2-P2A-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  TITLE     LV CRISPR ZNF865-targeting plasmids
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-U6-ZNF865-UbC-DsRed-P2A-Bsr
FEATURES             Location/Qualifiers
     source          1..10802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        112..491
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     646..666
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             709..889
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    936..1061
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1554..1787
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1972..2016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2314..2431
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2475..2508)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(2519..2594)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2616..2864)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2674..2693)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2844..2864)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2897..4108
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3945..3964
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4137..4811
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(4317..4337)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4535..4554
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4729..4752
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4821..4877
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5310..5343)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5368..5956
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5421..5441)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5839..5850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5959..5975)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5960..5976)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6481..6661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6687..6704)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6693..6917
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6963..7391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7050..7069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7260..7281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7400..7420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7405..7734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7585..7720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7647..7666
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7782..7829
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7848..8222
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8352..8473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8389..8408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8443..8462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8522..8538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8522..8538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8535..8557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8546..8562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8570..8576,8577..8594,8595..8600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8615..8636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8753..8770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8924..9512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9004..9023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9683..10474,10475..10543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10306..10325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10544..10648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10723..10742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
       61 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      121 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      181 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      241 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      301 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      361 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      421 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      481 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      541 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      601 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      661 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
      721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      901 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      961 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1021 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1081 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1141 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1201 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1261 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1321 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1381 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1441 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1501 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1561 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1621 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1681 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1741 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1801 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1861 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1921 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1981 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2041 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2101 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2161 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2221 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2281 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2341 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2401 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2461 agtttggtta attaataact tcgtatagca tacattatac gaagttatta aaaaaaaagc
     2521 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
     2581 tctagctcta aaacactcat ccatccttgt gcggcggtgt ttcgtccttt ccacaagata
     2641 tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta
     2701 aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt
     2761 tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct aacagccttg
     2821 tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttaatt aattaacccg
     2881 tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     2941 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     3001 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     3061 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     3121 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     3181 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     3241 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     3301 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     3361 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     3421 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     3481 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     3541 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     3601 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     3661 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     3721 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     3781 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     3841 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     3901 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     3961 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     4021 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     4081 ctggccgttt ttggcttttt tgttagacga agcttgggct gcaggtcgac tctagaatgg
     4141 atagcactga gaacgtcatc aagcccttca tgcgcttcaa ggtgcacatg gagggctccg
     4201 tgaacggcca cgagttcgag atcgagggcg agggcgaggg caagccctac gagggcaccc
     4261 agaccgccaa gctgcaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     4321 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
     4381 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     4441 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcaccttc atctaccacg
     4501 tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactctgg
     4561 gctgggagcc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     4621 acaaggcgct gaagctgaag ggcggcggcc actacctggt ggagttcaag tcaatctaca
     4681 tggccaagaa gcccgtgaag ctgcccggct actactacgt ggactccaag ctggacatca
     4741 cctcccacaa cgaggactac accgtggtgg agcagtacga gcgcgccgag gcccgccacc
     4801 acctgttcca gggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg
     4861 aagagaatcc tggaccgaag accttcaaca tctctcagca ggatctggag ctggtggagg
     4921 tcgccactga gaagatcacc atgctctatg aggacaacaa gcaccatgtc ggggcggcca
     4981 tcaggaccaa gactggggag atcatctctg ctgtccacat tgaggcctac attggcaggg
     5041 tcactgtctg tgctgaagcc attgccattg ggtctgctgt gagcaacggg cagaaggact
     5101 ttgacaccat tgtggctgtc aggcacccct actctgatga ggtggacaga tccatcaggg
     5161 tggtcagccc ctgtggcatg tgcagagagc tcatctctga ctatgctcct gactgctttg
     5221 tgctcattga gatgaatggc aagctggtca aaaccaccat tgaggaactc atccccctca
     5281 agtacaccag gaactaaagt tgataataga taacttcgta tagcatacat tatacgaagt
     5341 tatgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     5401 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5461 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5521 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5581 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5641 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5701 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5761 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5821 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5881 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5941 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     6001 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     6061 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     6121 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6181 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6241 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     6301 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     6361 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     6421 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     6481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6661 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6721 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6781 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6841 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6901 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6961 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7021 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7081 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7141 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7201 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7261 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7321 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7381 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7441 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7561 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7621 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     7681 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7741 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     7801 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     7861 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     7921 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     7981 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     8041 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     8101 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     8161 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     8221 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8281 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8341 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8461 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8521 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8641 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8701 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9361 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9961 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10681 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    10741 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    10801 ga
//
