LOCUS       sequence-483064-        4242 bp    DNA     circular SYN 13-AUG-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Rozario T, Quinn EB, Wang J, Davis RE, Newmark PA
  TITLE     Region-specific regulation of stem cell-driven regeneration in 
            tapeworms.
  JOURNAL   eLife. 2019 Sep 24;8. pii: 48958. doi: 10.7554/eLife.48958.
  PUBMED    31549962
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-09-24"; volume: "8. pii: 48958. doi"; pages: " 
            10.7554/eLife.48958"
COMMENT     Sequence Label: TR6_P1H04_hnf4
FEATURES             Location/Qualifiers
     source          1..4242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     246..265
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(394..462,463..1254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(612..631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1425..2013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1914..1933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2167..2184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2196..2243)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2257..2275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2279..2297
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2279..2296
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(3316..3334)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(3338..3356)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3370..3417
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             join(3824..4242,1..376)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3878..3897)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
       61 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
      121 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
      181 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
      241 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
      301 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
      361 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      601 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagccaggc aactatggat
     1201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2161 gagcgcagcg agtcagtgag cgaggaagca cgcgtcaaaa aacccctcaa gacccgttta
     2221 gaggccccaa ggggttatgc taggcgcgcc ggatcctaat acgactcact atagggagat
     2281 ttaggtgaca ctatagaagt gaccttagaa acaccgatcg atcacaacaa tgccttctat
     2341 tatgctcctg gtcttgagca ggttgaattc aactgcagtg aacaaccgat taataatcag
     2401 gaagagtatg acaaaaagct aaattcagaa caaggagtaa ctgactgtgg cataatgaca
     2461 atcgttaatg ggaatagcaa ctcaacactg agtggatatc caggcacgcc ggatgtggcc
     2521 cccaacacaa ggtccaaagg tcgacgagga tcccagaata ccgtgaaaga tgacgattcc
     2581 ttgatgggca actttggtgt gaaagttgat gagagaccac cttctggagc atcgaattta
     2641 ccggtatcta attgtctcat ttgcggtgat aaagcaactg gcaagcatta tggcgcttac
     2701 tcctgcgatg gatgcaaagg gttctttaga cgttcagtgc gtcgaaagca tacttatagt
     2761 tgtcgataca atcgaacttg tacaattgat aaagacatgc ggaatcaatg caggttctgc
     2821 cgtcttaaga aatgcttcag agttggaatg aacagagcag ccgttcaaca tgagagggat
     2881 aaaattagca atcggaggtc cttctatgat gcaccaggat tgaatggcac gttaatcgat
     2941 atcagcacac tattatgtgc ggaagtagag tctaaagaat ctgcagaacc aatcgaagcc
     3001 aaaaagatct cttcaagtgg tccactaacc ttgtcagata tttgttctgc ctttaaagtt
     3061 cacctctttc gtttgattaa ctgggcaaag caactggaat gtttttctag tctcgatatg
     3121 caagatcagt tggcactact gcggggaaat gccggagaat tattgctact aagcatagtg
     3181 tggcaagcaa tttgttcttc ttcacaacat acaaatgact ctggatcctt cgttgggcta
     3241 tttcaacgac tcaatgcggc ttttcttgga gttattaagg ttggaagtgg agagtatcct
     3301 gctgtgcaag gtctcccttt agtgagggtt aattctccct atagtgagtc gtattaggat
     3361 ccggcgcgcc tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgacg
     3421 cgtcgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3481 ttaacaaaat tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
     3541 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
     3601 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
     3661 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
     3721 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaatgatct gatggcgcag
     3781 gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3841 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3901 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3961 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     4021 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4081 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatccca
     4141 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4201 tgatccggct acctgcccat tcgaccacca agcgaaacat cg
//
