LOCUS       sequence-483067-        3904 bp    DNA     circular SYN 13-AUG-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Rozario T, Quinn EB, Wang J, Davis RE, Newmark PA
  TITLE     Region-specific regulation of stem cell-driven regeneration in 
            tapeworms.
  JOURNAL   eLife. 2019 Sep 24;8. pii: 48958. doi: 10.7554/eLife.48958.
  PUBMED    31549962
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-09-24"; volume: "8. pii: 48958. doi"; pages: " 
            10.7554/eLife.48958"
COMMENT     Sequence Label: TR8_P3H06_pogzl
FEATURES             Location/Qualifiers
     source          1..3904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..49)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        85..103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(784..802)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(785..802)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(806..824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      838..885
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(897..914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1068..1656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1148..1167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1827..2618,2619..2687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2450..2469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2705..3499)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2816..2835)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3426..3445
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagg cgcgccggat
       61 cctaatacga ctcactatag ggagaattaa ccctcactaa agggagacct tgacgatttt
      121 cgaaaccaac aagactgatg gtactgaagg aacccagttc tcagcatctg gtttctcctg
      181 tcggatctgt ggaaaagagg cagctagtga tctggcactt gcacatcatc tacagtccac
      241 acatcgtcaa cgtgaaatgc cctatgtgtg tcgcatctgc cttttccgtt cctctctcta
      301 tgaggatgct ctggagcatt tcaaaaaagt ccacaatggt tcaaatcatt tactctgttt
      361 ctactgcctt agatacttca ctcctactga cgcacgtgtt cctggtatcc ccttgacctc
      421 ccctactggc acatctggag gtgccgttag caccgcaggt ttaggagctg gtgtgggaca
      481 aacgcaggtg tatctgcagc acttgagaat gcatcaggtt cagcatcaac tccgacgctg
      541 tccatcatgt cgcttgaact ttacaaacaa atcggactac caggtgcatc ggcggttgga
      601 ccataaggcg ggtagcagct gtagtgcagc ggctgcggaa caacacctga aggaacttca
      661 acggcaacag caacaagttg aagagcaggc tcaaatgcat gaacaaggag aggagcagga
      721 acaaattatt gagcatatgg atgaacatgc tcatgagcag ccacagcata acataaggtc
      781 acttctatag tgtcacctaa atctccctat agtgagtcgt attaggatcc ggcgcgccta
      841 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgacgcg tgcttcctcg
      901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1501 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1861 gcgatctgtc tatttcgttc atccatagtt gcctggctcc ccgtcgtgta gataactacg
     1921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2701 caattcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     2761 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     2821 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     2881 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     2941 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     3001 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     3061 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     3121 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     3181 ggtgggatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     3241 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     3301 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     3361 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     3421 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     3481 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
     3541 ccctgcgcca tcagatcatt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     3601 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     3661 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     3721 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
     3781 gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gaattttgtt
     3841 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcga
     3901 cgcg
//
