LOCUS       sequence-483068-       11246 bp    DNA     circular SYN 13-AUG-2025
DEFINITION  Lentiviral expression vector encoding for multiplex regulation of 
            ACAN/Col2a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11246)
  TITLE     Bowles lab plasmids 2025
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-mU6-ACAN-hU6-Col2a1-hUbC-dsRed
FEATURES             Location/Qualifiers
     source          1..11246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..23
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(142..162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        147..476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      327..462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     389..408
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        524..571
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             590..964
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1094..1215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1131..1150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1185..1204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1264..1280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1264..1280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1277..1299)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1288..1304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1312..1318,1319..1336,1337..1342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1357..1378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1495..1512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1666..2254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1746..1765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2425..3216,3217..3285))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3048..3067
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3286..3390)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3465..3484)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3656..4035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4037..4235
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4190..4210
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4253..4433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4480..4605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5098..5331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5516..5560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5858..5975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(6020..6053)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6058..6371
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     6290..6310
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        6394..6469
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(6499..6574)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(6596..6844)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6654..6673)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6824..6844)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        6874..7088
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     7009..7028
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        7390..8601
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8438..8457
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      8648..8657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8654..9331
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(8834..8854)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9052..9071
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9246..9269
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9356..9944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9409..9429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9827..9838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9947..9963)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9948..9964)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10469..10649
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10675..10692)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10681..10905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10951..11246,1..133)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11038..11057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
       61 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      121 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
      181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
      241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      301 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
      421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      481 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
      541 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
      601 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
      661 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
      721 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
      781 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
      841 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
      901 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
      961 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     1021 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1081 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1201 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     1261 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1321 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1381 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1621 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1681 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1741 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1801 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1861 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1921 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1981 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2041 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2101 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2161 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2221 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2281 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2341 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2401 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2461 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2521 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2581 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2641 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2701 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2761 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2821 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2881 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2941 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3001 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3061 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3121 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3181 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3241 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3301 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3361 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3421 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     3481 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     3541 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     3601 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     3661 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3721 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3781 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3841 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3901 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3961 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4021 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4081 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4141 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4201 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
     4261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     4441 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     4501 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     4561 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4621 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4681 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4741 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4801 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4861 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     4921 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     4981 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     5041 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5101 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     5161 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     5221 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     5281 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     5341 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5401 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5461 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5521 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5581 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5641 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5701 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5761 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     5821 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     5881 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     5941 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     6001 atccagtttg gttaattaaa taacttcgta tagcatacat tatacgaagt tatgatagat
     6061 ccgacgccgc catctctagg cccgcgccgg ccccctcgca cagacttgtg ggagaagctc
     6121 ggctactccc ctgccccggt taatttgcat ataatatttc ctagtaacta tagaggctta
     6181 atgtgcgata aaagacagat aatctgttct ttttaatact agctacattt tacatgatag
     6241 gcttggattt ctataagaga tacaaatact aaattattat tttaaaaaac agcacaaaag
     6301 gaaactcacc ctaactgtaa agtaattgtg tgttttgaga ctataaatat cccttggaga
     6361 aaagccttgt ttggaccgag gggcggccga caggttttag agctagaaat agcaagttaa
     6421 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgtac
     6481 gctcagcgga gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
     6541 ccttatttta acttgctatt tctagctcta aaacccgctg ctcctttcta ccgccggtgt
     6601 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     6661 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     6721 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
     6781 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     6841 cctcttcctg cccgaccttg gatcgatccg cctgaacgct gacgtcatca acccgctcca
     6901 aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg
     6961 aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt
     7021 ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata agttctgtat
     7081 gagaccactc tttcccaggt cttcgagaag acccgaggta cccaagcggc gcacaagcta
     7141 tataaacctg aaggaagtct caactttaca cttaggtcaa gttgcttatc gtactagagc
     7201 ttcagcagga aatttaacta aaatctaatt taaccagcat agcaaatatc atttattccc
     7261 aaaatgctaa agtttgagat aaacggactt gatttccggc tgttttgaca ctatccagaa
     7321 tgccttgcag atgggtgggg catgctaaat actgcagaca attaattaac ccgtgtcggc
     7381 tccagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     7441 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
     7501 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     7561 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     7621 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     7681 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     7741 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc
     7801 tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg
     7861 agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg
     7921 gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc
     7981 gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct
     8041 tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct
     8101 ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg
     8161 ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct
     8221 cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg
     8281 gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct
     8341 ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt
     8401 ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg
     8461 cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca
     8521 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     8581 tttttggctt ttttgttaga cgaagcttgg gctgcaggtc gactctagag gatccccggg
     8641 taccggtgcc accatggata gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt
     8701 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggcaa
     8761 gccctacgag ggcacccaga ccgccaagct gcaggtgacc aagggcggcc ccctgccctt
     8821 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
     8881 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     8941 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     9001 caccttcatc taccacgtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat
     9061 gcagaagaag actctgggct gggagccctc caccgagcgc ctgtaccccc gcgacggcgt
     9121 gctgaagggc gagatccaca aggcgctgaa gctgaagggc ggcggccact acctggtgga
     9181 gttcaagtca atctacatgg ccaagaagcc cgtgaagctg cccggctact actacgtgga
     9241 ctccaagctg gacatcacct cccacaacga ggactacacc gtggtggagc agtacgagcg
     9301 cgccgaggcc cgccaccacc tgttccagta ggaattcgat atcaagctta tcgataatca
     9361 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     9421 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     9481 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     9541 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     9601 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     9661 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     9721 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     9781 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     9841 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     9901 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     9961 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
    10021 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
    10081 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10141 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
    10201 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
    10261 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
    10321 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
    10381 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
    10441 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
    10501 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10561 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10621 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
    10681 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10741 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10801 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10861 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    10921 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    10981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    11041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    11161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    11221 tgacgttgga gtccacgttc tttaat
//
