LOCUS       sequence-483110-        4164 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Allows for rap-1::mCherry entry into Gateway Vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Lamb R, Scales M, Watkins J, Werner M, Cherra SJ
  TITLE     A PDZ-RapGEF promotes synaptic development in C. elegans through a 
            Rap/Rac signaling pathway.
  JOURNAL   Development. 2025 Aug 1:dev.204678. doi: 10.1242/dev.204678.
  PUBMED    40748210
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2025 Aug 1:dev.204678. doi: 10.1242/dev.204678."
COMMENT     Sequence Label: pBD24
FEATURES             Location/Qualifiers
     source          1..4164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2043..2142)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2159..2178)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2160..2178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2183..2199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2183..2199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2630..3421
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3514..4102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4003..4022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttggactagt catatgggta ccatgcggga gtataagatt
      721 gttgtgctcg gatctggagg agtaggaaaa tccgcactga cggtacaatt cgtgcaggga
      781 attttcgtag agaagtatga cccaacaatt gaggatagtt acagaaagca agtggaagtc
      841 gacggccagc aatgtatgct tgagatactg gatacagcag gaacggagca attcacagcg
      901 atgcgtgatt tgtacatgaa gaatggacaa ggatttgttc tcgtttattc aattactgct
      961 caatcgacat tcaatgatct tatggatctt cgtgatcaga ttcttcgtgt caaagatacg
     1021 gacgaggttc caatgatttt ggtaggaaat aagtgtgatt tggaagacga acgagttgtt
     1081 ggaaaagatc agggacagaa tctcgccagg cagtttggat cagcattcct tgaaacctct
     1141 gcaaaagcaa agattaacgt cagcgaggtg ttctacgacc tcgttcgtca aataaaccga
     1201 cgttatcccg aatctggtcg acgccaggga caatcaaata agcagtgctg ctcgtgtgtc
     1261 atcatgggta ccagtggttc cggaggatct ggaggatctg gtatggtctc aaagggtgaa
     1321 gaagataaca tggcaattat taaagagttt atgcgtttca aggtgcatat ggagggatct
     1381 gtcaatgggc atgagtttga aattgaaggt gaaggagaag gccgaccata tgagggaaca
     1441 caaaccgcaa aactaaaggt aactaaaggc ggaccattac cattcgcctg ggacatcctc
     1501 tctccacagt tcatgtatgg aagtaaagct tatgttaaac atccggcaga tataccagat
     1561 tatttgaaac tttcattccc ggagggtttt aagtgggaac gcgtaatgaa ttttgaagac
     1621 ggaggagttg ttacagtgac gcaagactca agcctccaag atggagaatt tatttataaa
     1681 gtcaaacttc gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg
     1741 ggatgggaag cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt
     1801 aaacaacgtc ttaaattgaa agatggagga cattacgatg ctgaggtgaa gacaacttac
     1861 aaagccaaaa aaccagttca gctgccagga gcgtacaatg ttaatattaa actggatatc
     1921 acctcccaca acgaggatta cactatcgtt gagcaatatg aaagagctga agggcggcac
     1981 tcgacaggtg gcatggatga attgtataag tagcatatgg cggccgcgga agggcgaatt
     2041 cgacccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat caatttgttg
     2101 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2161 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2221 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     2281 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
     2341 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     2401 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2461 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2521 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2581 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     2641 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2701 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2761 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2821 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2881 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2941 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     3001 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     3061 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     3121 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3181 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3241 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3301 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3361 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3421 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3661 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3901 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4141 ttgctggcct tttgctcaca tgtt
//
