LOCUS       sequence-483130-       10315 bp    DNA     circular SYN 13-AUG-2025
DEFINITION  Blue_light_controlled, hTERT_promoter_driven release of the tTA 
            transactivator from cytoplasm to nucleus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10315)
  TITLE     Photoswitchable intein for light control of covalent protein binding
            and cleavage
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: phTERT-cp PS Intein tTA
FEATURES             Location/Qualifiers
     source          1..10315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1702..2637
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3289..3738
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     CDS             join(3826..4443,4444..4827)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3896..3915)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             6205..6258
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6259..6969
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6404..6422)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6672..6691
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7092..7213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7129..7148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7183..7202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7220..7675)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7357..7378)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7569..7588
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7702..7806
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7808..8165
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7808..7999
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7831..7851)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8016..8151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8200..8994
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8200..8994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8254..8273)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8864..8883
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9182..9201)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9226..9273
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9602..10190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10091..10110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcatcagctt ttcaaagaca cactaactgc acccataata ctggggtgtc ttctgggtat
       61 cagcgatctt cattgaatgc cgggaggcgt ttcctcgcca tgcacatggt gttaattact
      121 ccagcataat cttctgcttc catttcttct cttccctctt ttaaaattgt gttttctatg
      181 ttggcttctc tgcagagaac cagtgtaagc tacaacttaa cttttgttgg aacaaatttt
      241 ccaaaccgcc cctttgccct agtggcagag acaattcaca aacacagccc tttaaaaagg
      301 cttagggatc actaagggga tttctagaag agcgacccgt aatcctaagt atttacaaga
      361 cgaggctaac ctccagcgag cgtgacagcc cagggagggt gcgaggcctg ttcaaatgct
      421 agctccataa ataaagcaat ttcctccggc agtttctgaa agtaggaaag gttacattta
      481 aggttgcgtt tgttagcatt tcagtgtttg ccgacctcag ctacagcatc cctgcaaggc
      541 ctcgggagac ccagaagttt ctcgcccctt agatccaaac ttgagcaacc cggagtctgg
      601 attcctggga agtcctcagc tgtcctgcgg ttgtgccggg gccccaggtc tggaggggac
      661 cagtggccgt gtggcttcta ctgctgggct ggaagtcggg cctcctagct ctgcagtccg
      721 aggcttggag ccaggtgcct ggaccccgag gctgccctcc accctgtgcg ggcgggatgt
      781 gaccagatgt tggcctcatc tgccagacag agtgccgggg cccagggtca aggccgttgt
      841 ggctggtgtg aggcgcccgg tgcgcggcca gcaggagcgc ctggctccat ttcccaccct
      901 ttctcgacgg gaccgccccg gtgggtgatt aacagatttg gggtggtttg ctcatggtgg
      961 ggacccctcg ccgcctgaga acctgcaaag agaaatgacg ggcctgtgtc aaggagccca
     1021 agtcgcgggg aagtgttgca gggaggcact ccgggaggtc ccgcgtgccc gtccagggag
     1081 caatgcgtcc tcgggttcgt ccccagccgc gtctacgcgc ctccgtcctc cccttcacgt
     1141 ccggcattcg tggtgcccgg agcccgacgc cccgcgtccg gacctggagg cagccctggg
     1201 tctccggatc aggccagcgg ccaaagggtc gccgcacgca cctgttccca gggcctccac
     1261 atcatggccc ctccctcggg ttaccccaca gcctaggccg attcgacctc tctccgctgg
     1321 ggccctcgct ggcgtccctg caccctggga gcgcgagcgg cgcgcgggcg gggaagcgcg
     1381 gcccagaccc ccgggtccgc ccggagcagc tgcgctgtcg gggccaggcc gggctcccag
     1441 tggattcgcg ggcacagacg cccaggaccg cgcttcccac gtggcggagg gactggggac
     1501 ccgggcaccc gtcctgcccc ttcaccttcc agctccgcct cctccgcgcg gaccccgccc
     1561 cgtcccgacc cctcccgggt ccccggccca gccccctccg ggccctccca gcccctcccc
     1621 ttcctttccg cggccccgcc ctctcctcgc ggcgcgagtt tagagctggt ttagtgaacc
     1681 gtcagatccg ctagcaccat ggctggagac atgagagctg ccaacctttg gccaagcccg
     1741 ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg
     1801 gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga
     1861 cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt
     1921 cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag
     1981 gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc
     2041 atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga
     2101 aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc
     2161 cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat
     2221 tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc
     2281 caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg
     2341 accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg
     2401 cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc
     2461 ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc cactagccgt
     2521 ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca
     2581 tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct
     2641 gggggtggtg gatcaggagg tggtggatca ggaggaggtg gttctggtgg aggaggttct
     2701 accggtgaaa tgatgctgaa aaaaatcctg aaaatcgagg aactggatga acgcggtacc
     2761 ggcagcggag gtcatacctt gtatgcgccg gggggttatg acatcatggg ttacctcata
     2821 cagatcatga ataggccgaa cccacaagtg gaactcggac ccgtcgatac ctcctgcgct
     2881 ctcattctgt gtgaccttaa gcagaaggat acccctatcg tgtacgcctc cgaggcattt
     2941 ctgtacatga cagggtactc gaacgccgaa gtgctgggac ggaactgccg cttcctgcaa
     3001 agcccggatg gaatggtgaa gcctaagtca acccgcaaat acgtggactc caacactatc
     3061 aacaccatgc gcaaggccat tgaccgcaat gctgaggtgc aagtggaagt ggtgaacttc
     3121 aagaagaatg gacagcgctt cgtcaacttc ctgactatga tccccgtgcg cgacgagacc
     3181 ggcgaatatc ggtacagcat ggggtttcag tgtgagacag agggcggctc aggaggcagc
     3241 ggcggttctg gaggttccgg tggcggctca ggaggtagcg gagggtctca cactctttac
     3301 gcccctggag gatacgacat tatgggatat ttgattcaga ttatgaaccg cccaaaccct
     3361 caggtcgaac tggggcctgt ggacacgtca tgtgccctga tcctgtgcga tctgaagcaa
     3421 aaggacactc cgattgtcta cgcctcggaa gccttcttgt atatgaccgg atacagcaat
     3481 gcagaggtgc tcggtaggaa ctgcagattc ctgcagtccc ccgacgggat ggtgaaacca
     3541 aagtccactc gcaaatatgt ggactcgaac acgatcaata caatgcggaa ggccatcgac
     3601 cggaacgccg aggtccaggt ggaggtggtc aactttaaga agaacggcca gcggttcgtg
     3661 aactttctca ccatgattcc ggtccgggat gaaaccggag agtacagata ctccatggga
     3721 ttccagtgcg aaaccgaagg gtcaggatcc gaactgattg atattgaagt tagcggtaac
     3781 cacctgttct atgccaatga tattctgacc cataatagct cgagctctag attagataaa
     3841 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     3901 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     3961 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     4021 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     4081 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     4141 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     4201 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
     4261 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     4321 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     4381 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     4441 tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga gggcctgctc
     4501 gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg cctgtccttt
     4561 ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt cagcctgggg
     4621 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     4681 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     4741 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     4801 gcccttggaa ttgacgagta cggtgggact agtggagggt caggggctgg agggtcgggg
     4861 gcaggcggtt cgggagccgg aggatctgga gggggtggtt cgggtggtgg tggtagcggc
     4921 tactgcctgg acctgaagac ccaggtgcag accccccagg gcatgaagga gatcagcaac
     4981 atccaggtgg gcgacctggt gctgagcaac accggctaca acgaggtgct gaacgtgttc
     5041 cccaagagca agaagaagag ctacaagatc accctggagg acggcaagga gatcatctgc
     5101 agcgaggagc acctgttccc cacccagacc ggcgagatga acatcagcgg cggcctgaag
     5161 gagggcatgt gcctgtacgt gaagggcggc tccggaggag gtagtggcgg agggagtggt
     5221 gggagtggct ctggttcagg cagtggttct gcaggcgata tgcgggccgc taatttgtgg
     5281 ccttctcctt tgatgattaa gagaagtaaa aaaaattcct tggctctgag cttgaccgct
     5341 gatcaaatgg tgtccgctct cctcgacgcc gaacctccta tcttgtacag cgaatacgac
     5401 cccacacggc catttagcga ggcctcaatg atgggactgc ttacaaatct cgctgataga
     5461 gaactcgtgc atatgattaa ttgggccaaa cgggtccccg gattcgtcga cctgactctg
     5521 catgaccaag tgcatctctt ggagtgcgca tggctggaaa ttctcatgat cggcctggtg
     5581 tggcggagca tggaacatcc tgtcaaactg ctcttcgccc ccaatctgct gctggatcgg
     5641 aatcaaggca agtgcgtgga aggaatggtc gaaatttttg atatgctcct cgccaccagc
     5701 agcaggttta gaatgatgaa cctccaaggc gaagagttcg tctgtctgaa gagcatcatc
     5761 ctgctgaaca gcggggtcta taccttccta agtagtacat taaaaagttt ggaggaaaaa
     5821 gatcacattc atcgggtgct cgataaaatt accgataccc taattcatct catggctaaa
     5881 gccggactca cactccaaca acaacatcaa agactcgctc aactgttact gattctgagc
     5941 catattagac atatgagcaa taagggaatg gaacacctct attccatgaa atgtaaaaat
     6001 gtcgtccctc tgtacgatct cctcctcgaa gccgccgatg ctcataggct gcacgcccct
     6061 acctccagag gcggagccag cgtcgaagaa accgatcagt cccatctggc taccgccgga
     6121 agcacctcca gccacagcct tcagaaatac tatattaccg gcgaagccga aggctttccc
     6181 gctaccgccg aattcggtag cggcgagggc agaggaagtc tgctaacatg cggtgatgtc
     6241 gaggagaatc ctggcccaat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     6301 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     6361 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     6421 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     6481 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     6541 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     6601 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     6661 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     6721 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     6781 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     6841 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     6901 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     6961 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     7021 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7081 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7141 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7201 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7261 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7321 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7381 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7441 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7501 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7561 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7621 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     7681 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7741 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7801 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7861 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7921 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7981 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8041 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8101 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8161 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     8221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     8341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     8401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     8461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     8521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     8581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     8641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     8701 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     8761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     8941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     9001 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     9061 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9121 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     9181 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     9241 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     9301 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     9361 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     9421 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     9481 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9541 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10261 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaat
//
