LOCUS       sequence-483212-       11352 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  For expressing fusion proteins with N terminal TurboID for proximity
            labeling in plants .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Greenhalgh R, Horner W, Klimpel KA, Chatterjee S, Scarpin MR, 
            Brunkard JO
  TITLE     Seamless, modular binary vectors for plant cell and molecular 
            biology.
  JOURNAL   Plant Biotechnol J. 2025 Jun 26. doi: 10.1111/pbi.70233.
  PUBMED    40574375
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Jun 26. doi: 10.1111/pbi.70233."
COMMENT     Sequence Label: pAVEC0_N-TurboID
FEATURES             Location/Qualifiers
     source          1..11352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    661..685
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1583..1594
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2734..2753
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2734..2752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2777..3029)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3056..3847)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3167..3186)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3777..3796
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    5703..5727
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6160..6975
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6160..6975
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6233..6252)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7063..7651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7552..7571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7805..7822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7837..7977
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7903..7922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8063..8085
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8321..8515
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8581..9654)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10083..10712)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
       61 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
      121 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
      181 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
      241 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
      301 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
      361 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
      421 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
      481 tgacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
      541 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
      601 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
      661 gtttacccgc caatatatcc tgtcaagctt cgtctcaagc gatatacatg taaaaataac
      721 gtatataaaa cgacataatg caaaacttgc tgagcaagtt tcctgcgggg cccagttaaa
      781 acttgctgag caagtttcct gtggggccca cttaaaactt gctgagcaag tttcctgtgg
      841 ggcccactaa ctttactttt aataaagtaa agttacttta ataaagttgt ccccttctat
      901 tgaccagtca acaaatctta tctgaaagga atcttcttca ggaagtttcc cgctcaaaaa
      961 cgtcacatca ttctacagta cccttccacg tgtcacgttt tgattggttg cccatttttt
     1021 tcgggccatc cggtaatatt ataacggatg gcccttatga gagttgtact actatatata
     1081 gtactccaat actccaatag atatgttaat atgctaagcc acgtcatcgg agtccaatgc
     1141 ctcctactaa aagatttcgt attcaagcaa aaaacatatt tcttacatat cctcagtgtt
     1201 ctctttcaaa agaagaagct cttgagcaaa ttcaaggaat acaactttca tctaataaaa
     1261 aatatattaa aattgccaga gagctacacg aagatgggca acctcatctc cacgtcctgc
     1321 ttcaactcga aggaaaagtt cagatcacaa atatcagatt attcgacctg gtatccccaa
     1381 ccaggtcagc acatttccat ccgaacattc agggagctaa atccagctcc gacgtcaagt
     1441 cctacgtcga caaggacgga gacacaattg aatgggggga attccagatc gacggtagaa
     1501 gtgctagagg aggtcaacag acagctaacg actcatatgc caaggcgtta aacgcaactt
     1561 ctcttgaaca agcacttcaa atattgaagg aagaacaacc aaaggattac ttccttcaac
     1621 atcacaatct tttgaacaat gctcaaaaga tatttcagag ggcacctgat ccatggactc
     1681 cactatttcc tctgtcctca ttcacaaacg ttcctgagga aatgcaagaa tgggctgatg
     1741 catattttgg ggttgatgcc gctgcgcggc ctttaagata taatagtatc atagtagagg
     1801 gtgattcaag aacagggaag actatgtggg ctagatcttt aggggcccac aattacatca
     1861 cagggcattt agattttagc cttagaacat attatgatga agtggaatac aacgtcattg
     1921 atgacgtaga tcccacttac ttaaagatga aacactggaa gcaccttatt ggagcacaaa
     1981 aggagtggca gacaaactta aagtatggaa aaccacgtgt cattaaaggt ggtatcccct
     2041 gcattatatt atgcaatcca ggacctgaga gctcatacca acaatttctt gaaaaaccag
     2101 aaaatgaagc ccttaagtcc tggacattac ataattcaac cttctgcaaa ctccaaggtc
     2161 cgctctttaa taaccaagca gaagcatcct cgcaaggtga ctctaccctg taactgccac
     2221 ttcacaatac accatgagtg taatagagga ttttcgcaca ggagaaccta ttactctcca
     2281 tcaggcaaca aattccgtgg aattcgagag tgtaccgaat ccactgtata tgaaactcct
     2341 atggttcgag agatacgggc caatctatca actgaagata caaatccgat tcaactacaa
     2401 cctccggaga gcgttgaatc ttcacaagtg ctggatagag ctaacgataa ccggatcgaa
     2461 caggatattg actggacccc gtttcttgaa ggtcttgaaa aagagactag agatatactt
     2521 ggataatcta ggtttaattt gtattaataa tgtaattaga ggtttaaatc atgtcctgta
     2581 tgaagaattt aattttgtat caagtgtaat tcagaaccag agtgttgcaa tgaacttgta
     2641 ctaatttcat tattaataat aaatattata ccctctcccg ggattaccct gttatcccta
     2701 ccccgcgcgt tggccgattc attaatgcag gtttaatacg actcactata gggcgaatgg
     2761 aactggacgg gcccacgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     2821 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     2881 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     2941 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     3001 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctctccgc ggttatcaga
     3061 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     3121 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     3181 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     3241 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     3301 gatcctcgcc gtcgggcatt ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     3361 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     3421 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     3481 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     3541 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     3601 caacgtcgag cacagcagcg caaggtacgc ccgtcgtggc cagccacgat agccgcgctg
     3661 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccggtc
     3721 taccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     3781 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     3841 gttcaatcca ggaggaagcc attgttatat ctccttggat cttgctcgag ctaggtagca
     3901 attcccgagg ctgtagccga cgatgacgcg tcaggagagt tgtgatatca ttgtttgcct
     3961 ccctgcgttg tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt
     4021 tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc cctttcttgt
     4081 taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt
     4141 caccgacgac ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact
     4201 gatactcttc actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc
     4261 cgtattcggt gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt
     4321 ccagtacctt ctctgccgtt tccaaatcgc cgctttggac ataccatccg taataacggt
     4381 tcaggcacag cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta
     4441 cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg
     4501 cgaaatattc ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca
     4561 ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgctt
     4621 gctgagtttc cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt
     4681 cgaaaccaat gcctaaagag aggttaaagc cgacagcagc agtttcatca atcaccacga
     4741 tgccatgttc atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt
     4801 aggagttggc cccaatccag tccattaatg cgtggtcgtg caccatcagc acgttatcga
     4861 atcctttgcc acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt
     4921 tgtggttaat caggaactgt tcgcccttca ctgccactga ccggatgccg acgcgaagcg
     4981 ggtagatatc acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt
     5041 cacccggttg ccagaggtgc ggattcacca cttgcaaagt cccgctagtg ccttgtccag
     5101 ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct gacatcacca ttggccacca
     5161 cctgccagtc aacagacgcg tggttacagt cttgcgcgac atgcgtcacc acggtgatat
     5221 cgtccaccca ggtgttcggc gtggtgtaga gcattacgct gcgatggatt ccggcatagt
     5281 taaagaaatc atggaagtaa gactgctatt accctgttat ccctaattta aatactagtc
     5341 tgcaggcacg ccatggttat aagtacatat acatgtaaaa aaaatgtata taaaacgaca
     5401 taatgcaaaa cttgctgagc aagtttcctg cggggcccag ttaaaacttg ctgagcaagt
     5461 ttcctgtggg gcccactaac tttactttta ataaagtaaa gttactttaa taaagttgtc
     5521 cccttctatt gaccagtcaa caaatcttat ctgaaaggaa tcttcttcag gaagtttccc
     5581 gctcaaaaac gtcacatcat tctacagtac ccttccacgt gtcacgtttt gattggttgc
     5641 ccattttttt cgggccatcc ggtaatatta taacggctcc tgactgagac gaagcttgtt
     5701 atgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     5761 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     5821 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     5881 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     5941 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
     6001 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     6061 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     6121 aatactgtag aaaagaggaa ggaaataata agttctgtta tgagccatat tcaacgggaa
     6181 acgtcttgct caaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     6241 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct accgattgta tgggaagccc
     6301 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     6361 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     6421 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
     6481 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     6541 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     6601 cgactcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     6661 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     6721 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     6781 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     6841 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     6901 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     6961 gatgagtttt tctaatcacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt
     7021 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7081 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7141 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7201 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7261 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7321 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7381 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7441 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7501 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7561 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7621 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7681 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7741 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7801 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     7861 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     7921 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     7981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8041 tacagacaag ctgtgaccgt caccgggagc tgcatgtgtc agaggttttc accgtcatca
     8101 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     8161 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     8221 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     8281 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     8341 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     8401 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     8461 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     8521 gagtcctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     8581 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     8641 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     8701 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     8761 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     8821 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     8881 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     8941 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     9001 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     9061 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     9121 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     9181 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     9241 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     9301 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     9361 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     9421 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     9481 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     9541 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     9601 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     9661 tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca gccgcttacg
     9721 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     9781 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     9841 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     9901 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     9961 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
    10021 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
    10081 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
    10141 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
    10201 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
    10261 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
    10321 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
    10381 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
    10441 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
    10501 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
    10561 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
    10621 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
    10681 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    10741 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    10801 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    10861 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag aggtgcgcgg
    10921 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    10981 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    11041 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    11101 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    11161 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    11221 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    11281 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    11341 aatgccggcg aa
//
