LOCUS       sequence-483320-        9835 bp    DNA     circular SYN 14-AUG-2025
DEFINITION  H3K9ac FRET biosensor for real-time, single-cell monitoring of 
            H3K9ac levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Li S, Sun C, Harrison RES, Cheng L, Gao L, Liu L, Wang Y, Peng Q
  TITLE     Engineering FRET biosensor for H3K9 acetylation imaging in single 
            living cells
  JOURNAL   Med-X. 2024; 2, 26. doi: 10.1007/s44258-024-00032-4.
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Med-X."; 
            date: "2024"; volume: " 2, 26. doi"; pages: " 
            10.1007/s44258-024-00032-4"
COMMENT     Sequence Label: pSIn-H3K9ac BS
FEATURES             Location/Qualifiers
     source          1..9835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             118..751
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    798..923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1416..1649
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1834..1878
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2165..2282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2411..3589
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2642..3580
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3537..3557
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4761..4763,4764..4766,4767..5279)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(4806..4827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5067..5086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5414..5435
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5952..6526,6527..6529,6530..6538)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6119..6136)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6346..6365
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6558..7157
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6558..6577)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7054..7074
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7360..7593
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7744..7761)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7750..7974
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      8017..8026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        8070..8174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8175..8243,8244..9035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8393..8412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9206..9794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9695..9714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaccgcc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
       61 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
      121 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
      181 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      241 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      301 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      361 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      421 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      481 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      541 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      841 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      901 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      961 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1021 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1081 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1141 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1201 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1261 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1321 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1381 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1441 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1501 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1561 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1621 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1681 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1741 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1801 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1861 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1921 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     1981 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2041 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2101 agatccattc gattagtgaa cggatctcga cggtatcgcc acaaatggca gtattcatcc
     2161 acaattttaa aagaaagggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2221 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2281 ttcgggttta ttacagggac agcagagatc cactttggat cgataagctt tgcaaagatg
     2341 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     2401 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2461 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2521 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2581 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2641 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2701 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     2761 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     2821 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     2881 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     2941 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     3001 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     3061 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     3121 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     3181 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     3241 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     3301 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     3361 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     3421 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     3481 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     3541 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc
     3601 ttcgaactag tgccaccatg tccaacccta acaagcccaa gaggcagacc aaccaactgc
     3661 aatacctgct cagagtggtg ctcaagacac tatggaaaca ccagtttgca tggcctttcc
     3721 agcagcctgt ggatgccgtc aagctgaacc tccctgatta ctataagatc attaaaacgc
     3781 ctatggatat gggaacaata aagaagcgct tggaaaacaa ctattactgg aatgctcagg
     3841 aatgtatcca ggacttcaac actatgttta caaattgtta catctacaac aagcctggag
     3901 atgacatagt cttaatggca gaagctctgg aaaagctctt cttgcaaaaa ataaatgagc
     3961 tacccacaga agaaaccatg tctaaaggtg aagaattatt cactggtgtt gtcccaattt
     4021 tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag
     4081 gtgatgctac gtacggtaaa ttgaccttaa aattactctg tactactggt aaattgccag
     4141 ttccatggcc aaccttagtc actactttag gttatggtgt tcaatgtttt gctagatacc
     4201 cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag
     4261 aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg
     4321 aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta
     4381 acattttagg tcacaaattg gaatacaact ataactctca caatgtttac atcactgctg
     4441 acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt gaagatggtg
     4501 gtgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt
     4561 taccagacaa ccattactta tcctatcaat ctgccttatt caaagatcca aacgaaaaga
     4621 gagatcacat ggtcttgtta gaatttttga ctgctgctgg ttcaacttct ggctctggga
     4681 aaccaggaag cggcgaaggg tccaccaagg gaagcaccag tggttcaggt aagcctggtt
     4741 ctggtgaagg tagcactaaa atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4801 ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg tccggcgagg
     4861 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4921 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
     4981 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5041 tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5101 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5161 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
     5221 ccgccgacaa gcagaagaac ggcatcaagg cccacttcaa gatccgccac aacatcgagg
     5281 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5341 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5401 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgcacga accaagcaga
     5461 ctgcccgcaa atccaccggc ggtaaggcac cacggaagca gctggctacc aaagctgcta
     5521 gaaagagtgc tcctgcaaca gggggagtga aaaagcctca ccgatatcgc cctgggactg
     5581 tggcccttag ggagatcaga agataccaaa aaagcacaga gttgctgatc cggaagctgc
     5641 catttcaacg actggtgcgg gagatagccc aggactttaa gaccgatctt aggttccaat
     5701 cctcagctgt tatggcactc caggaggcca gtgaagctta cctcgttggg ttgtttgaag
     5761 acaccaacct gtgcgccatc cacgccaaaa gggtgacaat catgccgaag gatattcagc
     5821 tcgccagaag aatcagaggg gagagggctt agtaggaatt cgaagcttga gctcgagatc
     5881 tgagtccggt agcgctagtt cgaaggatcc gcatgcatct agggcggcca attccgcccc
     5941 tctccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     6001 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     6061 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     6121 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     6181 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     6241 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     6301 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     6361 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     6421 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6481 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca
     6541 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6601 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6661 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6721 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6781 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6841 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6901 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6961 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7021 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7081 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7141 gcaagcccgg tgcctgataa taggcggccg ctcgagacct agaaaaacat ggagcaatca
     7201 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7261 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7321 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7381 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7441 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7501 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7561 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7621 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattataata
     7681 gatttagcag gaattgaact aggagtggag cacacaggca aagttctaga gctcgctgat
     7741 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7801 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7861 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7921 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7981 aggcggaaag aaccagctgg gggcgcgccc ctcgaggccg ccatggtcat agctgtttga
     8041 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8101 tacattcaaa tatgtatccg ctcacgagac aataaccctg ataaatgctt caataatatt
     8161 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8221 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8281 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8341 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8401 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8461 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8521 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8581 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8641 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8701 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8761 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8821 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8881 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8941 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9001 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9061 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9121 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9181 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9241 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9301 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9361 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9421 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9481 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9541 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9601 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9661 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9721 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9781 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttctggcct tttgc
//
