LOCUS       sequence-483363-       11558 bp    DNA     circular SYN 14-AUG-2025
DEFINITION  FHA2-mutated FRET biosensor R605A; FRET biosensor for live cell 
            visualization of pSer22 Lamin A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Liu J, Li Q, Wang J, Qiu J, Zhou J, Peng Q
  TITLE     Genetically Encoded Fluorescence Resonance Energy Transfer Biosensor
            for Live-Cell Visualization of Lamin A Phosphorylation at Serine 22.
  JOURNAL   Biomater Res. 2024 Oct 22;28:0091. doi: 10.34133/bmr.0091. 
            eCollection 2024.
  PUBMED    39440272
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biomater 
            Res."; date: "2024-10-22"; volume: "28:0091. doi"; pages: " 
            10.34133/bmr.0091. eCollection 2024"
COMMENT     Sequence Label: pSIN-FHA2 R605A-Ypet-34-ECFP-hLaminA
FEATURES             Location/Qualifiers
     source          1..11558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             118..751
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    798..923
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1416..1649
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1834..1878
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2165..2282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2411..3589
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2642..3580
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3537..3557
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4882..4884,4885..4887,4888..5400)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(4927..4948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5188..5207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5535..5556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(7675..8249,8250..8252,8253..8261)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7842..7859)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8069..8088
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8281..8880
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8281..8300)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8777..8797
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9083..9316
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9467..9484)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9473..9697
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      9740..9749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        9793..9897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9898..9966,9967..10758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10116..10135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10929..11517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11418..11437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaccgcc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
       61 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
      121 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
      181 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
      241 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
      301 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
      361 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
      421 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
      481 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
      541 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      841 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      901 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      961 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1021 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1081 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1141 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1201 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1261 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1321 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1381 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1441 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1501 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1561 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1621 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1681 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1741 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1801 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1861 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1921 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     1981 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2041 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2101 agatccattc gattagtgaa cggatctcga cggtatcgcc acaaatggca gtattcatcc
     2161 acaattttaa aagaaagggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2221 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2281 ttcgggttta ttacagggac agcagagatc cactttggat cgataagctt tgcaaagatg
     2341 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     2401 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2461 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2521 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2581 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2641 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2701 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     2761 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     2821 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     2881 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     2941 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     3001 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     3061 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     3121 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     3181 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     3241 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     3301 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     3361 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     3421 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     3481 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     3541 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc
     3601 ttcgaagcta gcgccaccat gaagggtaat ggtaggtttt taactttaaa accattgcct
     3661 gacagcatta ttcaagaaag cctggagatt cagcaaggtg tgaatccatt tttcattggt
     3721 gcatccgagg attgcaattg taaaattgaa gacaataggt tgtctcgagt tcattgcttc
     3781 attttcaaaa agaggcatgc tgtaggcaaa agcatgtatg aatctccggc acaaggttta
     3841 gatgatattt ggtattgcca caccggaact aacgtgagct atttaaataa taaccgcatg
     3901 atacagggta cgaaattcct tttacaagac ggagatgaaa tcaagatcat ttgggataaa
     3961 aacaataaat ttgtcattgg ctttaaagtg gaaattaacg atactacagg tctgtttaac
     4021 gagggattag gtatgttaca agaacaaaga gtagtactta agcaaacagc cgaagaaaaa
     4081 gatttggtga aaaagttaat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt
     4141 ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa
     4201 ggtgatgcta cgtacggtaa attgacctta aaattactct gtactactgg taaattgcca
     4261 gttccatggc caaccttagt cactacttta ggttatggtg ttcaatgttt tgctagatac
     4321 ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa
     4381 gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt
     4441 gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt
     4501 aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta catcactgct
     4561 gacaaacaaa agaatggtat caaagctaac ttcaaaatta gacacaacat tgaagatggt
     4621 ggtgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg
     4681 ttaccagaca accattactt atcctatcaa tctgccttat tcaaagatcc aaacgaaaag
     4741 agagatcaca tggtcttgtt agaatttttg actgctgctg gttcaacttc tggctctggg
     4801 aaaccaggaa gcggcgaagg gtccaccaag ggaagcacca gtggttcagg taagcctggt
     4861 tctggtgaag gtagcactaa aatggtgagc aagggcgagg agctgttcac cggggtggtg
     4921 cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag
     4981 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5041 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
     5101 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5161 gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5221 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5281 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
     5341 accgccgaca agcagaagaa cggcatcaag gcccacttca agatccgcca caacatcgag
     5401 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5461 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5521 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccggggg aagcggcggg
     5581 accatggaga ccccgtccca gcggcgcgcc acccgcagcg gggcgcaggc cagctccact
     5641 ccgctgtcgc ccacccgcat cacccggctg caggagaagg aggacctgca ggagctcaat
     5701 gatcgcttgg cggtctacat cgaccgtgtg cgctcgctgg aaacggagaa cgcagggctg
     5761 cgccttcgca tcaccgagtc tgaagaggtg gtcagccgcg aggtgtccgg catcaaggcc
     5821 gcctacgagg ccgagctcgg ggatgcccgc aagacccttg actcagtagc caaggagcgc
     5881 gcccgcctgc agctggagct gagcaaagtg cgtgaggagt ttaaggagct gaaagcgcgc
     5941 aataccaaga aggagggtga cctgatagct gctcaggctc ggctgaagga cctggaggct
     6001 ctgctgaact ccaaggaggc cgcactgagc actgctctca gtgagaagcg cacgctggag
     6061 ggcgagctgc atgatctgcg gggccaggtg gccaagcttg aggcagccct aggtgaggcc
     6121 aagaagcaac ttcaggatga gatgctgcgg cgggtggatg ctgagaacag gctgcagacc
     6181 atgaaggagg aactggactt ccagaagaac atctacagtg aggagctgcg tgagaccaag
     6241 cgccgtcatg agacccgact ggtggagatt gacaatggga agcagcgtga gtttgagagc
     6301 cggctggcgg atgcgctgca ggaactgcgg gcccagcatg aggaccaggt ggagcagtat
     6361 aagaaggagc tggagaagac ttattctgcc aagctggaca atgccaggca gtctgctgag
     6421 aggaacagca acctggtggg ggccgcccac gaggagctgc agcagtcgcg catccgcatc
     6481 gacagcctct ctgcccagct cagccagctc cagaagcagc tggcagccaa ggaggcgaag
     6541 cttcgagacc tggaggactc actggcccgt gagcgggaca ccagccggcg gctgctggcg
     6601 gaaaaggagc gggagatggc cgagatgcgg gcaaggatgc agcagcagct ggacgagtac
     6661 caggagcttc tggacatcaa gctggccctg gacatggaga tccacgccta ccgcaagctc
     6721 ttggagggcg aggaggagag gctacgcctg tcccccagcc ctacctcgca gcgcagccgt
     6781 ggccgtgctt cctctcactc atcccagaca cagggtgggg gcagcgtcac caaaaagcgc
     6841 aaactggagt ccactgagag ccgcagcagc ttctcacagc acgcacgcac tagcgggcgc
     6901 gtggccgtgg aggaggtgga cgaggagggc aagtttgtcc ggctgcgcaa caagtccaat
     6961 gaggaccagt ccatgggcaa ttggcagatc aagcgccaga atggagatga tcccttgctg
     7021 acttaccggt tcccaccaaa gttcaccctg aaggctgggc aggtggtgac gatctgggct
     7081 gcaggagctg gggccaccca cagcccccct accgacctgg tgtggaaggc acagaacacc
     7141 tggggctgcg ggaacagcct gcgtacggct ctcatcaact ccactgggga agaagtggcc
     7201 atgcgcaagc tggtgcgctc agtgactgtg gttgaggacg acgaggatga ggatggagat
     7261 gacctgctcc atcaccacca cggctcccac tgcagcagct cgggggaccc cgctgagtac
     7321 aacctgcgct cgcgcaccgt gctgtgcggg acctgcgggc agcctgccga caaggcatct
     7381 gccagcggct caggagccca ggtgggcgga cccatctcct ctggctcttc tgcctccagt
     7441 gtcacggtca ctcgcagcta ccgcagtgtg gggggcagtg ggggtggcag cttcggggac
     7501 aatctggtca cccgctccta cctcctgggc aactccagcc cccgaaccca gagcccccag
     7561 aactgcagca tcatgtagga attcgaagct tgagctcgag atctgagtcc ggtagcgcta
     7621 gttcgaagga tccgcatgca tctagggcgg ccaattccgc ccctctcccc cccccccctc
     7681 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     7741 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     7801 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     7861 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     7921 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     7981 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     8041 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     8101 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     8161 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     8221 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag gcgaccttcc
     8281 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     8341 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     8401 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     8461 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     8521 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     8581 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     8641 cccgcgtggt tcctggccac cgtcggcgtt tcgcccgacc accagggcaa gggtctgggc
     8701 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     8761 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     8821 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     8881 taataggcgg ccgctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     8941 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     9001 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9061 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     9121 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9301 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     9361 aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag caggaattga
     9421 actaggagtg gagcacacag gcaaagttct agagctcgct gatcagcctc gactgtgcct
     9481 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     9541 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     9601 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     9661 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     9721 tgggggcgcg cccctcgagg ccgccatggt catagctgtt tgacgtcagg tggcactttt
     9781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9841 ccgctcacga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10141 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10981 ccgctaccag cggtggtttt tttgccggat caagagctac caactctttt tccgaaggta
    11041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    11101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11521 gccagcaacg cggccttttt acggttctgg ccttttgc
//
