LOCUS       sequence-483368-        1329 bp    DNA     linear   UNA 14-AUG-2025
DEFINITION  expresses TAPBPR-S104F-K211L-R270Q in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Sun Y, Papadaki GF, Devlin CA, Danon JN, Young MC, Winters TJ, 
            Burslem GM, Procko E, Sgourakis NG
  TITLE     Xeno interactions between MHC-I proteins and molecular chaperones 
            enable ligand exchange on a broad repertoire of HLA allotypes.
  JOURNAL   Sci Adv. 2023 Feb 24;9(8):eade7151. doi: 10.1126/sciadv.ade7151. 
            Epub 2023 Feb 24.
  PUBMED    36827371
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-24"; volume: "9(8):eade7151. doi"; pages: " 
            10.1126/sciadv.ade7151. Epub 2023 Feb 24"
COMMENT     Sequence Label: TAPBPR S104F K211L R270Q
FEATURES             Location/Qualifiers
     source          1..1329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             351..359
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1228..1251
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1258..1302
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1309..1326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtactc aggagggatg gtgcctgttg ctgtgcttgg ccttaagcgg agctgctgag
       61 accaaaccgc atcccgcgga gggtcagtgg cgcgcggtcg atgtggtgct cgactgcttc
      121 ctagtcaagg atggagccca ccgaggcgcc ctcgcatcca gtgaggatcg agctcgtgca
      181 tccttggtac tgaagcaagt gccggtactg gacgacggat cgctcgaaga cttcaccgac
      241 tttcagggcg gaacgctggc gcaggatgat ccaccaatta tattcgaagc tagtgtggat
      301 ctggtacaga tcccgcaggc cgaagctctg ctgcacgccg atagctccgg taaggaggtt
      361 acctgcgaga tcttccgcta ctttctgcag atgaccgaaa cgacggtgaa aaccgccgct
      421 tggttcatgg ccaatgtgca agtttctggc ggtggaccgt ccattagcct ggtcatgaaa
      481 acgccacggg tggccaagaa cgaagtgctg tggcatccga cgttgaatct gcctctctcc
      541 ccccaaggaa ctgtccgcac tgccgttgag ttccaggtga tgacacagac ccagagccta
      601 tcctttttac taggctcatc agcgagcttg gattgcggct tcagcatggc cccagggctc
      661 gacctcatat cggtggagtg gcggctccag cacctgggca gaggccagtt ggtctattcg
      721 tggacggccg gtcaaggtca agcggtgcgc aagggcgcaa cattggaacc ggcgcaactt
      781 ggcatggcca gggatgccag cctaaccttg cccggcctga ctattcagga cgagggcacc
      841 tatatttgtc aaatcacaac atctctctac caggctcagc agattataca actgaacatc
      901 caggcgtcgc ccaaggtgag gctgtcgctg gccaacgagg cccttcttcc cacactgatc
      961 tgtgatattg ccggctacta ccccttagac gtcgttgtca cctggactcg cgaggagctg
     1021 ggtggctcac ccgctcaggt tagcggggcg agtttttcgt ctcttcgtca atccgttgca
     1081 ggaacctact ccatctcgag ttcactaact gcagaacctg gctccgcagg agccacctat
     1141 acatgccagg tgacccacat ctctctggag gaacctctgg gggcctcgac gcaggttgtg
     1201 ccgccagagc gaagattgga aggtggcctg gaggtgctct tccagggacc cgggggtgga
     1261 ctgaacgata tttttgaagc ccaaaagatc gagtggcacg agggcggcca tcaccaccac
     1321 catcattaa
//
