LOCUS       sequence-483426-        6857 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  Archael (Asgard group archaeon SRVP18_Atabeyarchaeia-1) 16S rRNA 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6857)
  TITLE     Archaeal 16S rRNA expression
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Atabey1
FEATURES             Location/Qualifiers
     source          1..6857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        354..372
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2806..2853
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2891..2909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2916..2933)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2916..2932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2925..2947)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            3174..3283
                     /note="incP origin of transfer"
     rep_origin      3473..3901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3560..3579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3770..3791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             4245..5039
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4299..4318)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4909..4928
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(5057..5125,5126..5917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5275..5294)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6088..6676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6577..6596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6830..6847
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta aaccggttga tcctgccgga cccaaccgct atcggagtgg
      421 gactaagcca tgctagtcga gcgcctcgga ctatggcgag gcgcggcgta cggctcagta
      481 acacgtggtt aacctaacct ccggacgggg atacctccgg gaaactggag ctaatacccg
      541 ataggtgaat agttctggaa tggcttttca cccaaacggc tttgacacca tgtttgccaa
      601 cgtcgccaga ggatgggacc gcggccgatt aggctgttgg tggggtaacg gctcaccaaa
      661 ccgacaatcg gtacgggccg tgagagcggt agcccggaga tggggactga gacaaggccc
      721 caggtcctac ggggcgcagc aggcgcgaaa cctccgcaat gctggaaaca gcgacggggc
      781 tactccgagt gccgtccgct aagggcggct tttccacagt ataaaaagct gtgggaataa
      841 ggggagggca aggctggtgc cagccgccgc ggtaaaacca gctcctcgag tggttgggat
      901 gtttattggg cctaaagcgt ccgtagccgg ccttgtaagt ccttggctaa atccgtcagc
      961 ctaactgacg gcgtgctgag gatactgctt ggcttggggg cgagagaggc tgagggtact
     1021 actggggtag gggtgaaatc ctataatccc agtaggacca cctgtggcga aggcgttcag
     1081 ctggagcgcg cccgacggtg agggacgaaa gctaggggag cgaaccggat tagatacccg
     1141 ggtagtccta gctgtaaact atgcgggcta ggtgttggag cggccacgtg ctgctccagt
     1201 gccgaaggga agccgttaag cccgccgcct ggggagtacg gccgcaaggc tgaaacttaa
     1261 aggaattggc gggggagcac cacaaggggt gaagcctgcg gtttaattgg actcaacgcc
     1321 gggaaactta ccgggggcga cagcagaatg aaggccaggt tgacgacctt gcttgacaag
     1381 ctgagaggtg gtgcatgagg ccttcaggtc gtcctaaaac tcttcgcggc ttcctctaac
     1441 ctctctacga tgtgactcat agtcgtctct gtaggaaggc tgttgacaag tgttggcgag
     1501 ggagttcaaa tgaactcgct tgtgcctttc ttaggagggg atcatgacga tcgttgacga
     1561 caagaggaag tatgttctgg tcaaaacgaa gagtcttatc cgtcgtctgc gccaacaaga
     1621 ctgtggggct ctgcaaggag tatacaggca cgttgatgac ttggttgagt ggaatacctt
     1681 tcgagactgg gcgtatagag gctgcttccg gatgcctctc aacgtgttcg caaggttgaa
     1741 gaagacatat gagatcaagg acgtgccgcg agtggtcaca gagactcaat ggtacgcaga
     1801 gacacgcaag agagcagcaa gaataatgca cgaacgccac ccccataagg agtggctatc
     1861 gcgagcagcg aaggctggcg gtgaagctgt aaaagccaag tacgggatca ggtatctgac
     1921 aaaactctct ggatctgcca tacattcatc agccagatac cgatacaagg atggatatgg
     1981 aatagaatac cgtagccgcc tggaactgcg ggttgcggat ttgctgcgca aacatggata
     2041 caaattcgat tatgaaccgg agatagatgg atacatccct gacttcctaa tcaaccacac
     2101 aataatcgag gttagcggat actttcgcga ccacgattat ctttcaagac tccagatcaa
     2161 acttgcagac ctaaccacta agggttggga tatcatcgtt gtatgtgaac cgcgaaacaa
     2221 agctgctctc cagcaggcgc tcgtaggaat acgagtcaag gcaattatga caacggatga
     2281 gttcggacga tggctgtagg tctgcgaagc cgtcgtcagt tcgtgccgtg aggtgtcctg
     2341 tcaagtcagg caacgaacga gacccctgct tacagttgct accaggctta gaaatgggtc
     2401 tgagcacact gtagggactg ccggcgataa gctggaggaa ggagggggcc acggcaggtc
     2461 agtatgcccc gaatcccccg ggccacacgc gggctgcaat ggttgggaca atgggttccg
     2521 accccgaaag gggaaggtaa tcccgaaacc caatctcagt tgggatcgag ggctgcaact
     2581 cgccctcgtg aacatggaat ccctagtaat cgcgcgtcat catcgcgcgg tgaatactgt
     2641 ccctgctcct tgcacacatc gtgtctagga agcgcttagc gcgagtgtag accgcccgtc
     2701 gctccacccg agtcgggtta gggtgagacg tagccttttt ggccacgtcg aacccaggct
     2761 cggcaagggg ggagaagtcg taacaaggta gccgtaagac tccgtctagc ataacccctt
     2821 ggggcctcta aacgggtctt gaggggtttt ttgtgcatgc tcgagcggcc gccagtgtga
     2881 tggaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
     2941 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3001 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3061 aatggcgaat ggacgcgctg gaaggcagta caccttgata ggtgggctgc ccttcctggt
     3121 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     3181 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     3241 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     3301 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     3361 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcg
     3421 ctgcttccct gctgttttgt ggaatatcta ccgactggaa acaggcaaat gcaggaaccc
     3481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3901 tttaacaaaa ttcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc
     3961 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     4021 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     4081 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg
     4141 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     4201 ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4261 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4321 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4381 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     4441 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4501 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc
     4561 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4621 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4681 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4741 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     4801 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4861 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4921 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4981 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa
     5041 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5101 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5161 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5221 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5281 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5341 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5401 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5461 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5521 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5581 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5641 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5701 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5761 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5821 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5881 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5941 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6001 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6781 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6841 gagcgaggaa gcggaag
//
