LOCUS       sequence-483427-        5926 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  Archael (Bathyarchaeota JZ bin_7) 16S rRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5926)
  TITLE     Archaeal 16S rRNA expression
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BathyJZ7
FEATURES             Location/Qualifiers
     source          1..5926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        354..372
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1875..1922
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1960..1978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1985..2002)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1985..2001)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1994..2016)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            2243..2352
                     /note="incP origin of transfer"
     rep_origin      2542..2970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2629..2648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2839..2860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             3314..4108
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3368..3387)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3978..3997
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4126..4194,4195..4986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4344..4363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5157..5745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5646..5665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5899..5916
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta ctccggttga tcctgccgga ccccactgct atcggggtgg
      421 gactaagaca tgctagtcgt gcgcctccaa gccacggtgg aggcgcggcg cacagctcag
      481 taacacgtgg ctaacctacc cttgggacgg ggacaacccc gggaaactgg ggctaatccc
      541 cgataggtga ggactcctgg aatgggtcct cgccgaaagg gcgcctagac catgcttcta
      601 ggtgctgccc aaggatgggg ccgcggccga tcaggtagtt ggtgaggtaa cggctcacca
      661 agcctataac cggtacgggc cgtgggagcg ggagcccgga gatggacact gagacaaggg
      721 tccaggccct acggggcgca gcagtcgcga aacctccgca atacgcgaaa gcgtgacggg
      781 gccaccccga gtgccgtccg ctgaggacgg cttttcccca gtgtagccag ctgggggaat
      841 aaggggaggg caagtcgggt gtcagccgcc gcggtaatac ccgctccccg agtggtgggg
      901 acgcttattg ggcctaaagc atccgtagcc ggctgggtaa gtcccctgtt aaatccagcg
      961 attcaatcgc tggactgcgg gggatactgc ctagctaggg ggcgggagag gccggcggta
     1021 ttcccggggt aggggtgaaa tcttataatc ccgggaggac caccagtggc gaaggctgcc
     1081 ggctggaacg cgcccgacgg tgagggatga aagctggggg agcgaaccgg attagatacc
     1141 cgggtagtcc cagctgtaaa cgatgcgggc taggtgttgg cacggctacg agccgtgcca
     1201 gtgccgcagg gaagccgtta agcccgccgc ctggggagta cggccgcaag gctgaaactt
     1261 aaaggaattg gcgggggagc accacaaggg gtgaagcttg cggtttaatt ggagtcaacg
     1321 ccgggaacct taccggggga gacagcagga tgaaggccag attgaaggtc ttgccagaca
     1381 agctgagagg aggtgcatgg ccgtcgccag ttcgtgccgt gaggtgtcct gttaagtcag
     1441 gcaacgatcg agacccgcgc cccctgttgc taccggccat tgcgccgggc acactggggg
     1501 gactgccgcc gataaggcgg aggaaggagc gggccacggc aggtcagtat gccccgaatc
     1561 ccccgggcca cacgcgagct gcaatggcag ggacaatggg ttccgacccc gaaaggggga
     1621 ggcaatccct aaaccctgcc gtagttggga tcgagggctg caacccgccc tcgtgaacat
     1681 ggaatcccta gtaatcgcgc gtcaccatcg cgcggtgaat acgtccctgc tccttgcaca
     1741 caccgcccgt cgctccaccc gagcggggct tgggtgaggc gtggtcaagt tggctgcgtc
     1801 gaacccaagc ttcgtgaggg gggagaagtc gtaacaaggt ggccgtaggg gaacctgcgg
     1861 ccggatcacc tcctctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     1921 tgtgcatgct cgagcggccg ccagtgtgat ggaattcgcc ctatagtgag tcgtattaca
     1981 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2041 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2101 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgctgg aaggcagtac
     2161 accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc
     2221 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
     2281 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     2341 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     2401 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     2461 ccgaagcagg gttatgcagc ggaaaagcgc tgcttccctg ctgttttgtg gaatatctac
     2521 cgactggaaa caggcaaatg caggaaccct gtagcggcgc attaagcgcg gcgggtgtgg
     2581 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2641 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     2701 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2761 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2821 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2881 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     2941 agctgattta acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca agggctgcta
     3001 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     3061 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     3121 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     3181 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     3241 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     3301 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3361 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3421 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3481 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3541 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3601 agtgccgggg caggatctcc tgtcatccca ccttgctcct gccgagaaag tatccatcat
     3661 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3721 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3781 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3841 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3901 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3961 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4021 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4081 ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat
     4141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
