LOCUS       sequence-483460-       10804 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Retroviral vector encoding C-terminally FLAG tagged mouse GCN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S
  TITLE     Integrated stress response of vertebrates is regulated by four 
            eIF2alpha kinases.
  JOURNAL   Sci Rep. 2016 Sep 16;6:32886. doi: 10.1038/srep32886.
  PUBMED    27633668
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-16"; volume: "6:32886. doi"; pages: " 
            10.1038/srep32886"
COMMENT     Sequence Label: pMXs-mGCN2-FLAG-IP
FEATURES             Location/Qualifiers
     source          1..10804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1185..1250
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(1292..1866,1867..1869,1870..1878)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1459..1476)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1686..1705
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1879..2481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2378..2398
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2652..3121
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3251..3268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3422..4010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3502..3521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4174..4965,4966..5034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4797..4816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5035..5139)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5170..5686
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    5750..6107
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6166..6582
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6512..6534
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6550..6566
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    6592..6966
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     6904..6920
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      7021..7030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgagcagaa gggggatttg caagacttaa cgccaaccat caactcacta ataaaacaga
       61 aaacaggcgt tgctcagttg gtgaagtata gcttaaaaga cctcgaggac gtcgtcgggc
      121 tgctgaagaa acttggtgtg aagttacagg tctccatcaa cctgggcttg gtctacaagg
      181 tgcagcagca cactggcatc atcttccagt tcctggcatt cagcaaacgc aggcagaggg
      241 ttgtgcctga gatccttgcc gctggtggca gatacgacct gctgattccc aagttcagag
      301 gcccacagac tgtggggcca gtccccactg ctgtcggtgt cagcatagcc atagacaaga
      361 tatttgctgt tgtcctcaac atggaagagc ctgttacagt gagctcctgt gacctcctgg
      421 ttgtcagtgt tggccagatg tccatgtcca gggccatcaa cctaacccag aaactgtgga
      481 cagcgggcat cactgcagaa atcatgtatg actggtccca gtcccaggaa gagttacaag
      541 agtactgcag acaccatgaa atcacctatg tggctctggt ctccgataaa gaaggaagcc
      601 atgtcaaggt caagtccttt gagaaggaga ggcaaacaga aaagcgtgta ttggaatcgg
      661 atcttgtgga tcacgttatg cagaaactaa gaaccaaagt tggtgacgaa agaaatttca
      721 gagacgcttc tgataatctt gccgtacaaa ctctgaaggg gtcgttttct aatgcttcag
      781 gtttgtttga aatccatgga actacagtag tccccaacgt gattgttcta gcaccagaaa
      841 agctgtcagc cagcaccagg aggcgacatg agattcaggt gcaaacccga cttcagacta
      901 cacttgccaa cttacatcag aaaagcagtg aaattgaaat tttggctgtg gacctaccca
      961 aggaaacaat cttacagttt ctatcattag agtgggatgc tgatgaacag gcatttaaca
     1021 caactgtgaa gcagctgctg tcacgcctgc caaagcaaag atacctcaaa ctcgtctgcg
     1081 atgaaattta taacatcaaa gttgaaaaga aggtgtcagt gctgttcctg tacagctaca
     1141 gagatgacta ctacagaatc ctatttgtcc ccgaggccaa ggccgactac aaagaccatg
     1201 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag tagtgactgc
     1261 ggccgccgct acgtaaattc cgcccccccc ccccctctcc ctcccccccc cctaacgtta
     1321 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     1381 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     1441 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     1501 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     1561 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     1621 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     1681 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     1741 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     1801 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     1861 gataatatgg ccacaaccat ggttaccgag tacaagccca cggtgcgcct cgccacccgc
     1921 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     1981 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     2041 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     2101 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     2161 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     2221 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     2281 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     2341 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     2401 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     2461 acccgcaagc ccggtgcctg agtcgacgat aaaataaaag attttattta gtctccagaa
     2521 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     2581 ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac
     2641 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     2701 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     2761 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca
     2821 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     2881 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     2941 ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg
     3001 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     3061 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     3121 acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc
     3181 catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3241 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3301 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3361 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3421 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3541 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3601 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3661 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3721 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3781 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3841 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3901 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3961 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4021 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4081 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat
     4141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5161 acctgcataa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     5221 tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca
     5281 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     5341 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     5401 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     5461 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     5521 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     5581 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     5641 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     5701 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag
     5761 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
     5821 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg
     5881 gaacacccgg ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc
     5941 cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc ctaataggag
     6001 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
     6061 ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg
     6121 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc
     6181 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag
     6241 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt
     6301 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc
     6361 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg
     6421 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg
     6481 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct
     6541 cgatcctccc tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag
     6601 atcttatatg gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt
     6661 actaacagcc cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc
     6721 tggagacctc tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct
     6781 taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc
     6841 tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc
     6901 gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct
     6961 agactgccgg atctagctag ttaattaagg atcccagtgt ggtggtacgg gaattctaga
     7021 gccaccatgg ctgggggccg cggggcctct ggacgcggcc gggctgagcc acaggagagc
     7081 tattcgcagc gacaggacca cgagctgcag gccctggagg ccatttacgg ctcggacttc
     7141 caggatctgc ggccggatgc gcgcggacgg gtgagagagc ctcctgaaat caacttagtt
     7201 ctttaccctc agggcctagc tggtgaagaa gtatacgtgc aagtggaact gcaggttaaa
     7261 tgcccgccaa catacccaga tgtagttccc gaaatagagt taaaaaatgc taaaggcctg
     7321 tcgaatgaaa gtgttaattt gttaaaatca cacctggaag aactggccaa aaagcagtgt
     7381 ggagaggtga tgatatttga actggcacac catgtgcagt cgtttctcag cgagcataac
     7441 aagcccccac caaagtcttt ccatgaagaa atgctggaaa ggcaggctca ggagaagcag
     7501 cagaggttgc tggaggccag gcggaaggag gagcaggagc aacgtgaaat cctccacgag
     7561 attcagagaa ggaaagagga gatcaaggaa gagaagaaaa ggaaggaaat ggctaagcag
     7621 gaacgtttgg aaatcactag cttgacaaac caggactatg cctctaagag agacccagca
     7681 gggcacaggg cagctgccat cctccatgga ggctctcctg actttgtagg aaatggtaaa
     7741 gcccggacat actcctcagg aaggtccagg cgagaacgtc agtattctgt gtgtagtggt
     7801 gaaccctctc ctggctcgtg tgacatcctc catttctctg tgggtagccc tgatcagctc
     7861 atggtgcaca aagggagatg tgttggcagt gatgagcagc tgggaaaggt ggtatacaat
     7921 gctttggaaa cagccacggg cagctttgtc ttgctgcatg agtgggtcct tcagtggcag
     7981 aagatgggcc cttgccttac cagtcaagag aaagagaaga ttgacaagtg caaaaggcag
     8041 attcaaggag cagaaacaga attcagctcc ctagtgaaac tgagccatcc aaatatcgtc
     8101 cgctactttg caatgaactc cagagaggag gaggactcca ttgtgatcga cattctggca
     8161 gagcacgtca gtggcatctc tctggctaca cacctgagcc actcgggccc agtccccgcc
     8221 caccagctgc gcaagtacac agcccagctc ctggcaggcc tggattacct acacagcaac
     8281 tccgtggtgc acaaggttct gagcgcgtcc agtgtcttgg tagatgccga gggcactgtc
     8341 aagataacag actacagcat ctctaagcgt ctggcagaca tttgcaagga ggatgtattt
     8401 gagcaagctc gagttcgttt tagtgacagt gccctgcctt ataaaacagg aaagaaaggg
     8461 gatgtgtggc gtcttggcct cctgctgttg tctctcagcc aaggacagga gtgtggggag
     8521 tatccggtga ccatccccag tgacctgcca gctgacttcc aagacttcct gaagaagtgt
     8581 gtctgcctgg acgacaagga aagatggagc cctcagcagc tgctgaaaca cagctttata
     8641 aaccctcaac caaagctgcc tttggtggag cagagtcctg aagactctgg gggacaagac
     8701 tacattgaga ccgtcattcc cagcaaccag ctgcccagcg ctgcgttctt cagtgagaca
     8761 cagaaacagt tctcccggta cttcattgag tttgaagaac tacaactcct agggaaagga
     8821 gcttttggag ctgtcatcaa ggtgcaaaac aagcttgatg gctgctgcta tgctgtgaag
     8881 cgcatcccta tcaaccctgc aagcagacac ttccgccgta tcaagggcga ggtgacactg
     8941 ctatcgcgcc tgcaccatga gaacattgtg cgctactaca acgcctggat cgagcggcat
     9001 gagcgcccag cggtgccagg gacaccgccc ccagactgca caccccaggc ccaggacagc
     9061 ccagccacct acgggaaaac atcaggcgac actgaagaac tgggcagcgt ggaggctgca
     9121 gcaccgccac ccatcctcag cagctcggtg gagtggagca catctgcaga gcgttctacc
     9181 agcacccgct tcccagtcac gggccaggat tccagcagcg atgaggagga cgaggacgag
     9241 cgcgatggcg tcttctccca gtcctttcta cctgcttcag attctgacag tgacatcatc
     9301 tttgacaatg aagatgaaaa cagtaaaagt caaaatcagg atgaagactg caatcaaaag
     9361 gacggtagcc atgaggttga gccttcagtg acagctgaag ctgtgcacta cctttatatc
     9421 cagatggagt actgcgagaa gagcacgctg cgggacacca ttgaccaggg gctgttccga
     9481 gacaccagcc ggctctggag gcttttccga gagattctgg atggattagc ttatatccat
     9541 gagaaaggaa tgattcatcg cgacttgaag cctgtcaata tttttttgga ttctgatgac
     9601 catgtgaaaa taggtgactt tggcctggca acagaccatc tggccttcac tgctgaaggt
     9661 aaacaggacg accaggcagg tgatcgagtg attaagtcag acccttcagg ccatttgact
     9721 ggcatggttg gtactgctct gtatgtaagc cctgaggtcc aaggaagcac caagtctgca
     9781 tacaaccaga aagtggatct cttcagcctg ggaattatct tctttgagat gtcctatcac
     9841 ccgatggtga ctgcctcaga aagaattttc gttctcaacc aacttcgaga tcccacatcg
     9901 cccaagtttc cagacgactt cgatgatgga gaacatacaa agcagaaatc tgtcatctcc
     9961 tggctgttga accatgatcc agctaaacgg cccacggcca tggaactgct caagagtgag
    10021 ctgctccccc caccgcagat ggaggaatcc gagctgcatg aagtgctgca ccacacgctg
    10081 gccaacatag atgggaaggc ctaccgcacc atgatgagtc agatcttctg ccagcacatc
    10141 tcccccgcta tagactacac ctacgacagt gacatcctga agggcaactt cttgattcgt
    10201 acagccaaga tccagcagct tgtatgtgaa accatcgtcc gcgtctttaa aaggcatggc
    10261 gctgtccagc tgtgcacccc actgctgctt ccccgaaaca ggcaaatata tgagcacaac
    10321 gaagcggctt tgttcatgga tcacagcggc atgctggtga tgcttccgtt cgacctgcgg
    10381 gtcccttttg caagatatgt ggcaagaaat aacatactga atttaaaacg atactgcata
    10441 gagcgggtgt tcagacctcg gaagttagac cgatttcatc ccaaagaact cctggaatgt
    10501 gcatttgata tcgtcacttc taccaccaac agctctctgc ccactgccga aaccatctac
    10561 accatctatg aaataatcca ggagtttcct gcacttcagg aaaggaatta cagcatttac
    10621 ttgaaccata cgatgcttct gaaggcaata ctcctacact gtgggatccc ggaggacaag
    10681 ctcagccaag tctacgtcat tctgtatgat gctgtgacag agaagctgac taggagagaa
    10741 gtggaagcta aattctgtaa cctgtccttg tcttctaata gcctgtgtag actctacaag
    10801 ttca
//
