LOCUS       sequence-483496-        6767 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Mammalian astrocytes SypHer3s expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Lanin AA, Chebotarev AS, Pochechuev MS, Kelmanson IV, Kotova DA, 
            Bilan DS, Ermakova YG, Fedotov AB, Ivanov AA, Belousov VV, Zheltikov
            AM
  TITLE     Two- and three-photon absorption cross-section characterization for 
            high-brightness, cell-specific multiphoton fluorescence brain 
            imaging.
  JOURNAL   J Biophotonics. 2020 Mar;13(3):e201900243. doi: 
            10.1002/jbio.201900243. Epub 2020 Jan 9.
  PUBMED    31568649
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biophotonics."; date: "2020-03"; volume: "13(3):e201900243. doi"; 
            pages: " 10.1002/jbio.201900243. Epub 2020 Jan 9"
COMMENT     Sequence Label: pAAV_GFAP_SypHer3s_cyto
FEATURES             Location/Qualifiers
     source          1..6767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   673..802
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     complement(3451..3472)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3712..3731)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4186..4662
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4385..4404)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4702..4842
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4702..4831
                     /label=AAV2 ITR
     rep_origin      4917..5372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5004..5023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5214..5235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5389..5408)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5508..5530
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5568..5586)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5654..5758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5759..5827,5828..6619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
      721 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      781 aactccatca ctaggggttc ctgcggccgc acgcgtctta aggctgggag gttgaggctg
      841 cagtgagtcg tggttgcgcc actgcactcc agcctgggca acagtgagac cctgtctcaa
      901 aagacaaaaa aaaaaaaaaa aaaagaacat atcctggtgt ggagtagggg acgctgctct
      961 gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag acagccaggc
     1021 cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc tcctcttcat
     1081 gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac agtgcctgct
     1141 tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag cagggggctt
     1201 gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca gccccctagg
     1261 ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt cagcctgtgc
     1321 ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg ggggagagga
     1381 gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggactg ggcagggttc
     1441 tgaccctgtg ggaccagagt ggagggcgta gatggacctg aagtctccag ggacaacagg
     1501 gcccaggtct caggctccta gttgggccca gtggctccag cgtttccaaa cccatccatc
     1561 cccagaggtt cttcccatct ctccaggctg atgtgtggga actcgaggaa ataaatctcc
     1621 agtgggagac ggaggggtgg ccagggaaac ggggcgctgc aggaataaag acgagccagc
     1681 acagccagct catgtgtaac ggctttgtgg agctgtcaag gcctggtctc tgggagagag
     1741 gcacagggag gccagacaag gaaggggtga cctggaggga cagatccagg ggctaaagtc
     1801 ctgataaggc aagagagtgc cggccccctc ttgccctatc aggacctcca ctgccacata
     1861 gaggccatga ttgaccctta gacaaagggc tggtgtccaa tcccagcccc cagccccaga
     1921 actccaggga atgaatgggc agagagcagg aatgtgggac atctgtgttc aagggaagga
     1981 ctccaggagt ctgctggaaa tgaggcctag taggaaatga ggtggccctt gagggtacag
     2041 aacaggttca ttcttcgcca aattcccagc accttgcagg cacttacagc tgagtgagat
     2101 aatgcctggg ttatgaaatc aaaaagttgg aaagcaggtc agaggtcatc tggtacagcc
     2161 cttccttccc tttttttttt ttttttttgt gagacaaggt ctctctctgt tgcccaggct
     2221 ggagtggcgc aaacacagct cactgcagcc tcaacctact gggctcaagc aatcctccag
     2281 cctcagcctc ccaaagtgct gggattacaa gcatgagcca ccccactcag ccctttcctt
     2341 cctttttaat tgatgcataa taattgtaag tattcatcat ggtccaacca accctttctt
     2401 gacccacctt cctagagaga gggtcctctt gcttcagcgg tcagggcccc agacccatgg
     2461 tctggctcca ggtaccacct gcctcatgca ggagttggcg tgcccaggaa gctctgcctc
     2521 tgggcacagt gacctcagtg gggtgagggg agctctcccc atagctgggc tgcggcccaa
     2581 ccccaccccc tcaggctatg ccagggggtg ttgccagggg cacccgggca tcgccagtct
     2641 agcccactcc ttcataaagc cctcgcatcc caggagcgag cagagccaga gcaggttgga
     2701 gaggagacgc atcacctccg ctgctcgcgg gaattcgcca ccatgtccgg accgctgcac
     2761 attggtttga ttcccacagt tggaccgtac ctgctaccgc atattatccc tatgctgcac
     2821 cagacctttc caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg
     2881 caactggaca gcggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca
     2941 ttcattgaag tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg
     3001 tgggcgaacc gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg
     3061 gaagatggtc actctttgcg cgatcaggca atgtccgccg gcttcaacag cgacaacgtc
     3121 tatatcatgg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3181 gtcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3241 ggccccgtgc tgctgcccga caaccactac ctgagcttcc agtccgtcct gagcaaagac
     3301 cccaacgaga agcgcgatca catggtcctg caggagttcg tgaccgccgc cgggatcact
     3361 ctcggcatgg acgagctgta caacgtggat ggcggtagcg gtggcaccgg cagcaagggc
     3421 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3481 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3541 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctc
     3601 ggctacggcc tgaagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     3661 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3721 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3781 ctgaagggca tcggcttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3841 ggcaccggtt tctgttttga agccggggcg gatgaagata cacactttcg cgcgaccagc
     3901 ctggaaactc tgcgcaacat ggtggcggca ggtagcggga tcactttact gccagcgctg
     3961 gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc tgccgtgcat taagccggaa
     4021 ccacgccgca ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag
     4081 cagctggcag aggccatccg cgcaagaatg gatggccatt tcgataaagt tttaaaacag
     4141 gcggtttaaa agcttgcctc gagcagcgct gctcgagaga tctacgggtg gcatccctgt
     4201 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     4261 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     4321 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     4381 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     4441 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     4501 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     4561 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     4621 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
     4681 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4741 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4801 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4861 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4921 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4981 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5041 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5101 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     5161 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5221 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     5281 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5341 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     5401 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5461 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5521 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccg
//
