LOCUS       sequence-483536-        5645 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Mammalian neurons HyPer7 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Kelmanson IV, Shokhina AG, Kotova DA, Pochechuev MS, Ivanova AD, 
            Kostyuk AI, Panova AS, Borodinova AA, Solotenkov MA, Stepanov EA, 
            Raevskii RI, Moshchenko AA, Pak VV, Ermakova YG, van Belle GJC, 
            Tarabykin V, Balaban PM, Fedotov IV, Fedotov AB, Conrad M, Bogeski 
            I, Katschinski DM, Doeppner TR, Bahr M, Zheltikov AM, Belousov VV, 
            Bilan DS
  TITLE     In vivo dynamics of acidosis and oxidative stress in the acute phase
            of an ischemic stroke in a rodent model.
  JOURNAL   Redox Biol. 2021 Nov 3;48:102178. doi: 10.1016/j.redox.2021.102178.
  PUBMED    34773835
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Redox 
            Biol."; date: "2021-11-3"; volume: "48:102178. doi"; pages: " 
            10.1016/j.redox.2021.102178"
COMMENT     Sequence Label: pAAV_hSyn1_HyPer7_cyto
FEATURES             Location/Qualifiers
     source          1..5645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        145..448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        456..903
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     1527..1548
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1657..1678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1918..1937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2392..2868
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2591..2610)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2908..3048
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2908..3037
                     /label=AAV2 ITR
     rep_origin      3123..3578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3210..3229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3420..3441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3595..3614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3714..3736
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3774..3792)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3860..3964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3965..4033,4034..4825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4183..4202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4996..5584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5485..5504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      421 ctacgtatta gtcatcgcta ttaccatggc tcgagagtgc aagtgggttt taggaccagg
      481 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
      541 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
      601 cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
      661 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
      721 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
      781 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
      841 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
      901 caggaattcg ccaccatgca cctggctaat gaggagcaaa acgaattgga aggtgctttt
      961 aagctgggct tgatctttac cgtcgccccg tacttgctgc ctaagttgat cgtcagcctg
     1021 cgccgcaccg ccccgaagat gcctttgatg ttggaggaga attacaccca cacccttacc
     1081 gagtcactga aacgcggcga cgttgacgcc atcattgtgg cagagccgtt tcaagagccg
     1141 ggtattgtga ctgaaccttt gtatgacgag ccgtttttcg ttatcgttcc gaagggccat
     1201 agcttcgagg agttggatgc cgttagccct cgcatgttgg gcgaagaaca agttctgctt
     1261 ctgaccgagg gcaactgtat gcgtgaccag gtgcttagct ccgccggctt caacagcaac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggccaactt caagatccgc
     1381 cacaacgtcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcttccagtc cgtcctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaac gtggatggcg gtagcggtgg caccggcagc
     1621 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1681 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1741 accctgaagc tgatctgcac cactggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1801 accctcagct acggcctgaa gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1861 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1921 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1981 atcgagctga agggcatcgg cttcaaggag gacggcaaca tcctggggca caagctggag
     2041 tacaacggcc ccagttgcag cgagcttgcc gccaaacagc gcattcaggg tctcaccaac
     2101 acccttcaag gctcaagcat caataccatc cgccacatgg ttgccagtgg cttggcaatc
     2161 agcgtcttgc ctgccactgc ccttaccgaa aacgaccaca tgctgtttag cattatcccg
     2221 tttgaaggca ccccgcctag ccgccgcgtt gtcctggcct atcgccgcaa ctttgtccgc
     2281 cctaaggccc tttcagccat gaaagccgca attatgcaaa gccaactgca tggtgttagc
     2341 ttcatctgcg attgaaagct tgcctcgagc agcgctgctc gagagatcta cgggtggcat
     2401 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2461 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2521 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2581 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2641 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2701 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2761 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2821 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag
     2881 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     2941 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     3001 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     3061 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     3121 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3181 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3241 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3301 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     3361 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3421 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     3481 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     3541 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     3601 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3661 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3721 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     3781 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5641 catgt
//
