LOCUS       sequence-483546-       11833 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Expresses MMLVgag–dCas9-KOX1 for producing RENDER-dCas9-KOX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, 
            Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins 
            SE, Conklin BR, Nunez JK
  TITLE     Programmable epigenome editing by transient delivery of CRISPR 
            epigenome editor ribonucleoproteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 
            10.1101/2024.11.26.625496.
  PUBMED    39651312
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 
            10.1101/2024.11.26.625496."
COMMENT     Sequence Label: pCMV-MMLVgag-dCas9-KOX1
FEATURES             Location/Qualifiers
     source          1..11833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1161..1616)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1298..1319)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1510..1529
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1743..1765
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1757..1774
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1758..1774
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1784..1803
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1784..1802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1923..2302
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2303..2506
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2457..2477
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2503..2527
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2618..3190
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3288..3704
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3634..3656
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3672..3688
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4917..4943
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4956..4982
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4995..5021
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5055..5078
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5112..5132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5133..5156,5157..5159,5160..7646,7647..7649,
                     7650..9233)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9237..9263
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9243..9269
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             9282..9302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9309..9329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9369..10064
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             10110..10295
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             10338..10358
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10364..10383)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10429..10484
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10483..10502)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10842..10858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10842..10858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10855..10877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10866..10882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10890..10896,10897..10914,10915..10920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10935..10956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11073..11090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11244..11832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11324..11343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1201 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1261 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1321 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1381 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1441 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1501 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1561 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     1621 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1681 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1741 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
     1801 gggcgaattg gagctccacc gcggtggcgg ccgctctaga gagcttggcc cattgcatac
     1861 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
     1921 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1981 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2041 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2101 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2161 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2221 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2281 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2341 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2401 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2461 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2521 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     2581 atccagcctc cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg
     2641 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     2701 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     2761 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     2821 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     2881 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     2941 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     3001 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     3061 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     3121 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     3181 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga
     3241 attcctcgag ggccggatcc gaattcctgt atttgtctga aaacatgggc cagactgtta
     3301 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     3361 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     3421 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     3481 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     3541 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     3601 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     3661 ctcgatcctc cctttatcca gccctcactc cttctctagg cgccaaacct aaacctcaag
     3721 ttctttctga cagtgggggg ccgctcatcg acctacttac agaagacccc ccgccttata
     3781 gggacccaag accaccccct tccgacaggg acggaaatgg tggagaagcg acccctgcgg
     3841 gagaggcacc ggacccctcc ccaatggcat ctcgcctacg tgggagacgg gagccccctg
     3901 tggccgactc cactacctcg caggcattcc ccctccgcgc aggaggaaac ggacagcttc
     3961 aatactggcc gttctcctct tctgaccttt acaactggaa aaataataac ccttcttttt
     4021 ctgaagatcc aggtaaactg acagctctga tcgagtctgt cctcatcacc catcagccca
     4081 cctgggacga ctgtcagcag ctgttgggga ctctgctgac cggagaagaa aaacaacggg
     4141 tgctcttaga ggctagaaag gcggtgcggg gcgatgatgg gcgccccact caactgccca
     4201 atgaagtcga tgccgctttt cccctcgagc gcccagactg ggattacacc acccaggcag
     4261 gtaggaacca cctagtccac tatcgccagt tgctcctagc gggtctccaa aacgcgggca
     4321 gaagccccac caatttggcc aaggtaaaag gaataacaca agggcccaat gagtctccct
     4381 cggccttcct agagagactt aaggaagcct atcgcaggta cactccttat gaccctgagg
     4441 acccagggca agaaactaat gtgtctatgt ctttcatttg gcagtctgcc ccagacattg
     4501 ggagaaagtt agagaggtta gaagatttaa aaaacaagac gcttggagat ttggttagag
     4561 aggcagaaaa gatctttaat aaacgagaaa ccccggaaga aagagaggaa cgtatcagga
     4621 gagaaacaga ggaaaaagaa gaacgccgta ggacagagga tgagcagaaa gagaaagaaa
     4681 gagatcgtag gagacataga gagatgagca agctattggc cactgtcgtt agtggacaga
     4741 aacaggatag acagggagga gaacgaagga ggtcccaact cgatcgcgac cagtgtgcct
     4801 actgcaaaga aaaggggcac tgggctaaag attgtcccaa gaaaccacga ggacctcggg
     4861 gaccaagacc ccagacctcc ctcctgaccc tagatgactc tggcggctca cttcaactgc
     4921 ctccacttga aagactgaca ctgggatcat tacaattacc tcctttagaa cgattaacac
     4981 tcggttcact acagcttccg cctcttgaga gattgacatt aacgtccacg ctgctaatgg
     5041 agaactcgtc tggagattac aaagacgatg acgataagaa acggacagcc gacggaagcg
     5101 agttcgagtc accaaagaag aagcggaaag tcgacaagaa gtattctatc ggactggcca
     5161 tcgggactaa tagcgtcggg tgggccgtga tcactgacga gtacaaggtg ccctctaaga
     5221 agttcaaggt gctcgggaac accgaccggc attccatcaa gaaaaatctg atcggagctc
     5281 tcctctttga ttcaggggag accgctgaag caacccgcct caagcggact gctagacggc
     5341 ggtacaccag gaggaagaac cggatttgtt accttcaaga gatattctcc aacgaaatgg
     5401 caaaggtcga cgacagcttc ttccataggc tggaagaatc attcctcgtg gaagaggata
     5461 agaagcatga acggcatccc atcttcggta atatcgtcga cgaggtggcc tatcacgaga
     5521 aatacccaac catctaccat cttcgcaaaa agctggtgga ctcaaccgac aaggcagacc
     5581 tccggcttat ctacctggcc ctggcccaca tgatcaagtt cagaggccac ttcctgatcg
     5641 agggcgacct caatcctgac aatagcgatg tggataaact gttcatccag ctggtgcaga
     5701 cttacaacca gctctttgaa gagaacccca tcaatgcaag cggagtcgat gccaaggcca
     5761 ttctgtcagc ccggctgtca aagagccgca gacttgagaa tcttatcgct cagctgccgg
     5821 gtgaaaagaa aaatggactg ttcgggaacc tgattgctct ttcacttggg ctgactccca
     5881 atttcaagtc taatttcgac ctggcagagg atgccaagct gcaactgtcc aaggacacct
     5941 atgatgacga tctcgacaac ctcctggccc agatcggtga ccaatacgcc gaccttttcc
     6001 ttgctgctaa gaatctttct gacgccatcc tgctgtctga cattctccgc gtgaacactg
     6061 aaatcaccaa ggcccctctt tcagcttcaa tgattaagcg gtatgatgag caccaccagg
     6121 acctgaccct gcttaaggca ctcgtccggc agcagcttcc ggagaagtac aaggaaatct
     6181 tctttgacca gtcaaagaat ggatacgccg gctacatcga cggaggtgcc tcccaagagg
     6241 aattttataa gtttatcaaa cctatccttg agaagatgga cggcaccgaa gagctcctcg
     6301 tgaaactgaa tcgggaggat ctgctgcgga agcagcgcac tttcgacaat gggagcattc
     6361 cccaccagat ccatcttggg gagcttcacg ccatccttcg gcgccaagag gacttctacc
     6421 cctttcttaa ggacaacagg gagaagattg agaaaattct cactttccgc atcccctact
     6481 acgtgggacc cctcgccaga ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag
     6541 aaactatcac tccctggaac ttcgaagagg tggtggacaa gggagccagc gctcagtcat
     6601 tcatcgaacg gatgactaac ttcgataaga acctccccaa tgagaaggtc ctgccgaaac
     6661 attccctgct ctacgagtac tttaccgtgt acaacgagct gaccaaggtg aaatatgtca
     6721 ccgaagggat gaggaagccc gcattcctgt caggcgaaca aaagaaggca attgtggacc
     6781 ttctgttcaa gaccaataga aaggtgaccg tgaagcagct gaaggaggac tatttcaaga
     6841 aaattgaatg cttcgactct gtggagatta gcggggtcga agatcggttc aacgcaagcc
     6901 tgggtaccta ccatgatctg cttaagatca tcaaggacaa ggattttctg gacaatgagg
     6961 agaacgagga catccttgag gacattgtcc tgactctcac tctgttcgag gaccgggaaa
     7021 tgatcgagga gaggcttaag acctacgccc atctgttcga cgataaagtg atgaagcaac
     7081 ttaaacggag aagatatacc ggatggggac gccttagccg caaactcatc aacggaatcc
     7141 gggacaaaca gagcggaaag accattcttg atttccttaa gagcgacgga ttcgctaatc
     7201 gcaacttcat gcaacttatc catgatgatt ccctgacctt taaggaggac atccagaagg
     7261 cccaagtgtc tggacaaggt gactcactgc acgagcatat cgcaaatctg gctggttcac
     7321 ccgctattaa gaagggtatt ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga
     7381 tgggtcgcca taaaccagag aacattgtca tcgagatggc cagggaaaac cagactaccc
     7441 agaagggaca gaagaacagc agggagcgga tgaaaagaat tgaggaaggg attaaggagc
     7501 tcgggtcaca gatccttaaa gagcacccgg tggaaaacac ccagcttcag aatgagaagc
     7561 tctatctgta ctaccttcaa aatggacgcg atatgtatgt ggaccaagag cttgatatca
     7621 acaggctctc agactacgac gtggacgcca tcgtccctca gagcttcctc aaagacgact
     7681 caattgacaa taaggtgctg actcgctcag acaagaaccg gggaaagtca gataacgtgc
     7741 cctcagagga agtcgtgaaa aagatgaaga actattggcg ccagcttctg aacgcaaagc
     7801 tgatcactca gcggaagttc gacaatctca ctaaggctga gaggggcgga ctgagcgaac
     7861 tggacaaagc aggattcatt aaacggcaac ttgtggagac tcggcagatt actaaacatg
     7921 tcgcccaaat ccttgactca cgcatgaata ccaagtacga cgaaaacgac aaacttatcc
     7981 gcgaggtgaa ggtgattacc ctgaagtcca agctggtcag cgatttcaga aaggactttc
     8041 aattctacaa agtgcgggag atcaataact atcatcatgc tcatgacgca tatctgaatg
     8101 ccgtggtggg aaccgccctg atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg
     8161 gagactacaa ggtctacgac gtgcgcaaga tgattgccaa atctgagcag gagatcggaa
     8221 aggccaccgc aaagtacttc ttctacagca acatcatgaa tttcttcaag accgaaatca
     8281 cccttgcaaa cggtgagatc cggaagaggc cgctcatcga gactaatggg gagactggcg
     8341 aaatcgtgtg ggacaagggc agagatttcg ctaccgtgcg caaagtgctt tctatgcctc
     8401 aagtgaacat cgtgaagaaa accgaggtgc aaaccggagg cttttctaag gaatcaatcc
     8461 tccccaagcg caactccgac aagctcattg caaggaagaa ggattgggac cctaagaagt
     8521 acggcggatt cgattcacca actgtggctt attctgtcct ggtcgtggct aaggtggaaa
     8581 aaggaaagtc taagaagctc aagagcgtga aggaactgct gggtatcacc attatggagc
     8641 gcagctcctt cgagaagaac ccaattgact ttctcgaagc caaaggttac aaggaagtca
     8701 agaaggacct tatcatcaag ctcccaaagt atagcctgtt cgaactggag aatgggcgga
     8761 agcggatgct cgcctccgct ggcgaacttc agaagggtaa tgagctggct ctcccctcca
     8821 agtacgtgaa tttcctctac cttgcaagcc attacgagaa gctgaagggg agccccgagg
     8881 acaacgagca aaagcaactg tttgtggagc agcataagca ttatctggac gagatcattg
     8941 agcagatttc cgagttttct aaacgcgtca ttctcgctga tgccaacctc gataaagtcc
     9001 ttagcgcata caataagcac agagacaaac caattcggga gcaggctgag aatatcatcc
     9061 acctgttcac cctcaccaat cttggtgccc ctgccgcatt caagtacttc gacaccacca
     9121 tcgaccggaa acgctatacc tccaccaaag aagtgctgga cgccaccctc atccaccaga
     9181 gcatcaccgg actttacgaa actcggattg acctctcaca gctcggaggg gatgcctatc
     9241 cctatgacgt gcccgattat gccagcctgg gcagcggctc ccccaagaaa aaacgcaagg
     9301 tggaagatcc taagaaaaag cggaaagtgg acggcattgg tagtgggagc aacggcagca
     9361 gcggatccag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     9421 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     9481 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     9541 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     9601 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     9661 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     9721 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     9781 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     9841 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     9901 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     9961 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
    10021 ccagatactg cgacctccct agcaaactgg ggcacaagct taatggcggt ggcggaggga
    10081 tggatgctaa gtcactaact gcctggtccc ggacactggt gaccttcaag gatgtatttg
    10141 tggacttcac cagggaggag tggaagctgc tggacactgc tcagcagatc gtgtacagaa
    10201 atgtgatgct ggagaactat aagaacctgg tttccttggg ttatcagctt actaagccag
    10261 atgtgatcct ccggttggag aagggagaag agccctctgg cggctcaaaa agaaccgccg
    10321 acggcagcga attcgagccc aagaagaagc ggaaagtctg agatcttttt ccctctgcca
    10381 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
    10441 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
    10501 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
    10561 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
    10621 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
    10681 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
    10741 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
    10801 tatggagatc cctcgacgga tcggccgcaa ttcgtaacca tgtcatagct gtttcctgtg
    10861 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10921 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10981 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    11041 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11101 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11161 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11221 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11281 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11341 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11401 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11461 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11521 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11581 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11641 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11701 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11761 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11821 aaaggatctc aag
//
