LOCUS       sequence-483548-       11917 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Expresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, 
            Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins 
            SE, Conklin BR, Nunez JK
  TITLE     Programmable epigenome editing by transient delivery of CRISPR 
            epigenome editor ribonucleoproteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 
            10.1101/2024.11.26.625496.
  PUBMED    39651312
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 
            10.1101/2024.11.26.625496."
COMMENT     Sequence Label: pCMV-MMLVgag-dCas9-ZIM3
FEATURES             Location/Qualifiers
     source          1..11917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1785..1804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1924..2303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2304..2507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2458..2478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2504..2528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2619..3191
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3289..3705
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3635..3657
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3673..3689
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4918..4944
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4957..4983
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4996..5022
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5056..5079
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5113..5133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5134..5157,5158..5160,5161..7647,7648..7650,
                     7651..9234)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9238..9264
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9244..9270
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             9283..9303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9310..9330
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9370..10065
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             10423..10443
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10449..10468)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10514..10569
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10568..10587)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10927..10943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10927..10943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10940..10962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10951..10967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10975..10981,10982..10999,11000..11005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11020..11041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11158..11175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11329..11917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11409..11428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt ggagctccac cgcggtggcg gccgctctag agagcttggc ccattgcata
     1861 cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat
     1921 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1981 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2041 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2101 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2161 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2221 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2281 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2341 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2401 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2461 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2521 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     2581 gatccagcct ccggtcgacc gatcctgaga acttcagggt gagtttgggg acccttgatt
     2641 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     2701 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     2761 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     2821 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     2881 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     2941 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     3001 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     3061 ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     3121 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     3181 ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     3241 aattcctcga gggccggatc cgaattcctg tatttgtctg aaaacatggg ccagactgtt
     3301 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     3361 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     3421 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     3481 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     3541 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     3601 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     3661 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccaaacc taaacctcaa
     3721 gttctttctg acagtggggg gccgctcatc gacctactta cagaagaccc cccgccttat
     3781 agggacccaa gaccaccccc ttccgacagg gacggaaatg gtggagaagc gacccctgcg
     3841 ggagaggcac cggacccctc cccaatggca tctcgcctac gtgggagacg ggagccccct
     3901 gtggccgact ccactacctc gcaggcattc cccctccgcg caggaggaaa cggacagctt
     3961 caatactggc cgttctcctc ttctgacctt tacaactgga aaaataataa cccttctttt
     4021 tctgaagatc caggtaaact gacagctctg atcgagtctg tcctcatcac ccatcagccc
     4081 acctgggacg actgtcagca gctgttgggg actctgctga ccggagaaga aaaacaacgg
     4141 gtgctcttag aggctagaaa ggcggtgcgg ggcgatgatg ggcgccccac tcaactgccc
     4201 aatgaagtcg atgccgcttt tcccctcgag cgcccagact gggattacac cacccaggca
     4261 ggtaggaacc acctagtcca ctatcgccag ttgctcctag cgggtctcca aaacgcgggc
     4321 agaagcccca ccaatttggc caaggtaaaa ggaataacac aagggcccaa tgagtctccc
     4381 tcggccttcc tagagagact taaggaagcc tatcgcaggt acactcctta tgaccctgag
     4441 gacccagggc aagaaactaa tgtgtctatg tctttcattt ggcagtctgc cccagacatt
     4501 gggagaaagt tagagaggtt agaagattta aaaaacaaga cgcttggaga tttggttaga
     4561 gaggcagaaa agatctttaa taaacgagaa accccggaag aaagagagga acgtatcagg
     4621 agagaaacag aggaaaaaga agaacgccgt aggacagagg atgagcagaa agagaaagaa
     4681 agagatcgta ggagacatag agagatgagc aagctattgg ccactgtcgt tagtggacag
     4741 aaacaggata gacagggagg agaacgaagg aggtcccaac tcgatcgcga ccagtgtgcc
     4801 tactgcaaag aaaaggggca ctgggctaaa gattgtccca agaaaccacg aggacctcgg
     4861 ggaccaagac cccagacctc cctcctgacc ctagatgact ctggcggctc acttcaactg
     4921 cctccacttg aaagactgac actgggatca ttacaattac ctcctttaga acgattaaca
     4981 ctcggttcac tacagcttcc gcctcttgag agattgacat taacgtccac gctgctaatg
     5041 gagaactcgt ctggagatta caaagacgat gacgataaga aacggacagc cgacggaagc
     5101 gagttcgagt caccaaagaa gaagcggaaa gtcgacaaga agtattctat cggactggcc
     5161 atcgggacta atagcgtcgg gtgggccgtg atcactgacg agtacaaggt gccctctaag
     5221 aagttcaagg tgctcgggaa caccgaccgg cattccatca agaaaaatct gatcggagct
     5281 ctcctctttg attcagggga gaccgctgaa gcaacccgcc tcaagcggac tgctagacgg
     5341 cggtacacca ggaggaagaa ccggatttgt taccttcaag agatattctc caacgaaatg
     5401 gcaaaggtcg acgacagctt cttccatagg ctggaagaat cattcctcgt ggaagaggat
     5461 aagaagcatg aacggcatcc catcttcggt aatatcgtcg acgaggtggc ctatcacgag
     5521 aaatacccaa ccatctacca tcttcgcaaa aagctggtgg actcaaccga caaggcagac
     5581 ctccggctta tctacctggc cctggcccac atgatcaagt tcagaggcca cttcctgatc
     5641 gagggcgacc tcaatcctga caatagcgat gtggataaac tgttcatcca gctggtgcag
     5701 acttacaacc agctctttga agagaacccc atcaatgcaa gcggagtcga tgccaaggcc
     5761 attctgtcag cccggctgtc aaagagccgc agacttgaga atcttatcgc tcagctgccg
     5821 ggtgaaaaga aaaatggact gttcgggaac ctgattgctc tttcacttgg gctgactccc
     5881 aatttcaagt ctaatttcga cctggcagag gatgccaagc tgcaactgtc caaggacacc
     5941 tatgatgacg atctcgacaa cctcctggcc cagatcggtg accaatacgc cgaccttttc
     6001 cttgctgcta agaatctttc tgacgccatc ctgctgtctg acattctccg cgtgaacact
     6061 gaaatcacca aggcccctct ttcagcttca atgattaagc ggtatgatga gcaccaccag
     6121 gacctgaccc tgcttaaggc actcgtccgg cagcagcttc cggagaagta caaggaaatc
     6181 ttctttgacc agtcaaagaa tggatacgcc ggctacatcg acggaggtgc ctcccaagag
     6241 gaattttata agtttatcaa acctatcctt gagaagatgg acggcaccga agagctcctc
     6301 gtgaaactga atcgggagga tctgctgcgg aagcagcgca ctttcgacaa tgggagcatt
     6361 ccccaccaga tccatcttgg ggagcttcac gccatccttc ggcgccaaga ggacttctac
     6421 ccctttctta aggacaacag ggagaagatt gagaaaattc tcactttccg catcccctac
     6481 tacgtgggac ccctcgccag aggaaatagc cggtttgctt ggatgaccag aaagtcagaa
     6541 gaaactatca ctccctggaa cttcgaagag gtggtggaca agggagccag cgctcagtca
     6601 ttcatcgaac ggatgactaa cttcgataag aacctcccca atgagaaggt cctgccgaaa
     6661 cattccctgc tctacgagta ctttaccgtg tacaacgagc tgaccaaggt gaaatatgtc
     6721 accgaaggga tgaggaagcc cgcattcctg tcaggcgaac aaaagaaggc aattgtggac
     6781 cttctgttca agaccaatag aaaggtgacc gtgaagcagc tgaaggagga ctatttcaag
     6841 aaaattgaat gcttcgactc tgtggagatt agcggggtcg aagatcggtt caacgcaagc
     6901 ctgggtacct accatgatct gcttaagatc atcaaggaca aggattttct ggacaatgag
     6961 gagaacgagg acatccttga ggacattgtc ctgactctca ctctgttcga ggaccgggaa
     7021 atgatcgagg agaggcttaa gacctacgcc catctgttcg acgataaagt gatgaagcaa
     7081 cttaaacgga gaagatatac cggatgggga cgccttagcc gcaaactcat caacggaatc
     7141 cgggacaaac agagcggaaa gaccattctt gatttcctta agagcgacgg attcgctaat
     7201 cgcaacttca tgcaacttat ccatgatgat tccctgacct ttaaggagga catccagaag
     7261 gcccaagtgt ctggacaagg tgactcactg cacgagcata tcgcaaatct ggctggttca
     7321 cccgctatta agaagggtat tctccagacc gtgaaagtcg tggacgagct ggtcaaggtg
     7381 atgggtcgcc ataaaccaga gaacattgtc atcgagatgg ccagggaaaa ccagactacc
     7441 cagaagggac agaagaacag cagggagcgg atgaaaagaa ttgaggaagg gattaaggag
     7501 ctcgggtcac agatccttaa agagcacccg gtggaaaaca cccagcttca gaatgagaag
     7561 ctctatctgt actaccttca aaatggacgc gatatgtatg tggaccaaga gcttgatatc
     7621 aacaggctct cagactacga cgtggacgcc atcgtccctc agagcttcct caaagacgac
     7681 tcaattgaca ataaggtgct gactcgctca gacaagaacc ggggaaagtc agataacgtg
     7741 ccctcagagg aagtcgtgaa aaagatgaag aactattggc gccagcttct gaacgcaaag
     7801 ctgatcactc agcggaagtt cgacaatctc actaaggctg agaggggcgg actgagcgaa
     7861 ctggacaaag caggattcat taaacggcaa cttgtggaga ctcggcagat tactaaacat
     7921 gtcgcccaaa tccttgactc acgcatgaat accaagtacg acgaaaacga caaacttatc
     7981 cgcgaggtga aggtgattac cctgaagtcc aagctggtca gcgatttcag aaaggacttt
     8041 caattctaca aagtgcggga gatcaataac tatcatcatg ctcatgacgc atatctgaat
     8101 gccgtggtgg gaaccgccct gatcaagaag tacccaaagc tggaaagcga gttcgtgtac
     8161 ggagactaca aggtctacga cgtgcgcaag atgattgcca aatctgagca ggagatcgga
     8221 aaggccaccg caaagtactt cttctacagc aacatcatga atttcttcaa gaccgaaatc
     8281 acccttgcaa acggtgagat ccggaagagg ccgctcatcg agactaatgg ggagactggc
     8341 gaaatcgtgt gggacaaggg cagagatttc gctaccgtgc gcaaagtgct ttctatgcct
     8401 caagtgaaca tcgtgaagaa aaccgaggtg caaaccggag gcttttctaa ggaatcaatc
     8461 ctccccaagc gcaactccga caagctcatt gcaaggaaga aggattggga ccctaagaag
     8521 tacggcggat tcgattcacc aactgtggct tattctgtcc tggtcgtggc taaggtggaa
     8581 aaaggaaagt ctaagaagct caagagcgtg aaggaactgc tgggtatcac cattatggag
     8641 cgcagctcct tcgagaagaa cccaattgac tttctcgaag ccaaaggtta caaggaagtc
     8701 aagaaggacc ttatcatcaa gctcccaaag tatagcctgt tcgaactgga gaatgggcgg
     8761 aagcggatgc tcgcctccgc tggcgaactt cagaagggta atgagctggc tctcccctcc
     8821 aagtacgtga atttcctcta ccttgcaagc cattacgaga agctgaaggg gagccccgag
     8881 gacaacgagc aaaagcaact gtttgtggag cagcataagc attatctgga cgagatcatt
     8941 gagcagattt ccgagttttc taaacgcgtc attctcgctg atgccaacct cgataaagtc
     9001 cttagcgcat acaataagca cagagacaaa ccaattcggg agcaggctga gaatatcatc
     9061 cacctgttca ccctcaccaa tcttggtgcc cctgccgcat tcaagtactt cgacaccacc
     9121 atcgaccgga aacgctatac ctccaccaaa gaagtgctgg acgccaccct catccaccag
     9181 agcatcaccg gactttacga aactcggatt gacctctcac agctcggagg ggatgcctat
     9241 ccctatgacg tgcccgatta tgccagcctg ggcagcggct cccccaagaa aaaacgcaag
     9301 gtggaagatc ctaagaaaaa gcggaaagtg gacggcattg gtagtgggag caacggcagc
     9361 agcggatcca gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
     9421 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     9481 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     9541 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
     9601 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
     9661 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     9721 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
     9781 ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac
     9841 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat
     9901 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
     9961 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
    10021 gccagatact gcgacctccc tagcaaactg gggcacaagc ttaatggcgg tggcggaggg
    10081 atgaacaatt cccagggaag agtgaccttc gaggatgtca ctgtgaactt cacccagggg
    10141 gagtggcagc ggctgaatcc cgaacagaga aacttgtaca gggatgtgat gctggagaat
    10201 tacagcaacc ttgtctctgt gggacaaggg gaaaccacca aacccgatgt gatcttgagg
    10261 ttggaacaag gaaaggagcc atggttggag gaagaggaag tgctgggaag tggccgtgca
    10321 gaaaaaaatg gggacattgg agggcagatt tggaagccaa aggatgtgaa agagagtctc
    10381 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagcggaaa
    10441 gtctgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
    10501 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
    10561 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
    10621 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
    10681 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
    10741 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
    10801 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
    10861 ccagtcatag ctgtccctct tctcttatgg agatccctcg acggatcggc cgcaattcgt
    10921 aaccatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10981 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    11041 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    11101 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11521 tcatagctca cgctgtaggt atctcagttc ggtgtgggtc gttcgctcca agctgggctg
    11581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11761 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11881 caagcagcag attacgcgca gaaaaaaagg atctcaa
//
