LOCUS       sequence-483550-        5611 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Mammalian neurons SypHer3s expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Lanin AA, Chebotarev AS, Pochechuev MS, Kelmanson IV, Kotova DA, 
            Bilan DS, Ermakova YG, Fedotov AB, Ivanov AA, Belousov VV, Zheltikov
            AM
  TITLE     Two- and three-photon absorption cross-section characterization for 
            high-brightness, cell-specific multiphoton fluorescence brain 
            imaging.
  JOURNAL   J Biophotonics. 2020 Mar;13(3):e201900243. doi: 
            10.1002/jbio.201900243. Epub 2020 Jan 9.
  PUBMED    31568649
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biophotonics."; date: "2020-03"; volume: "13(3):e201900243. doi"; 
            pages: " 10.1002/jbio.201900243. Epub 2020 Jan 9"
COMMENT     Sequence Label: pAAV_hSyn1_SypHer3s_cyto
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        145..448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        456..903
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(1623..1644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1884..1903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2358..2834
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2557..2576)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2874..3014
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2874..3003
                     /label=AAV2 ITR
     rep_origin      3089..3544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3176..3195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3386..3407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3561..3580)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3680..3702
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3740..3758)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3826..3930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3931..3999,4000..4791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4149..4168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4962..5550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5451..5470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      421 ctacgtatta gtcatcgcta ttaccatggc tcgagagtgc aagtgggttt taggaccagg
      481 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
      541 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
      601 cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
      661 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
      721 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
      781 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
      841 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
      901 caggaattcg ccccatgtcc ggaccgctgc acattggttt gattcccaca gttggaccgt
      961 acctgctacc gcatattatc cctatgctgc accagacctt tccaaagctg gaaatgtatc
     1021 tgcatgaagc acagacccac cagttactgg cgcaactgga cagcggcaaa ctcgattgcg
     1081 tgatcctcgc gctggtgaaa gagagcgaag cattcattga agtgccgttg tttgatgagc
     1141 caatgttgct ggctatctat gaagatcacc cgtgggcgaa ccgcgaatgc gtaccgatgg
     1201 ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg tcactctttg cgcgatcagg
     1261 caatgtccgc cggcttcaac agcgacaacg tctatatcat ggccgacaag cagaagaacg
     1321 gcatcaaggc caacttcaag atccgccaca acgtcgagga cggcagcgtg cagctcgccg
     1381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1441 acctgagctt ccagtccgtc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1501 tgcaggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaacgtgg
     1561 atggcggtag cggtggcacc ggcagcaagg gcgaggagct gttcaccggg gtggtgccca
     1621 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1681 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     1741 ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg cctgaagtgc ttcgcccgct
     1801 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1861 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1921 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcggcttc aaggaggacg
     1981 gcaacatcct ggggcacaag ctggagtaca acggcaccgg tttctgtttt gaagccgggg
     2041 cggatgaaga tacacacttc cgcgcgacca gcctggaaac tctgcgcaac atggtggcgg
     2101 caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc aaacgcgatg
     2161 gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc ctggtttatc
     2221 gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa
     2281 tggatggcca tttcgataaa gttttaaaac aggcggttta aaagcttgcc tcgagcagcg
     2341 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     2401 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     2461 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     2521 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     2581 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     2641 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     2701 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     2761 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     2821 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     2881 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2941 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3001 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3061 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3301 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3421 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     3481 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3541 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3601 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3661 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3721 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3781 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4141 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5581 ctggcctttt gctggccttt tgctcacatg t
//
