LOCUS       sequence-483569-        5796 bp    DNA     circular SYN 15-AUG-2025
DEFINITION  Retroviral expression of human SOX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     Sequence Label: pMXs-hSOX2
FEATURES             Location/Qualifiers
     source          1..5796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    906..930
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1921..1945)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1996..2370
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2042..2058)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(2380..2796)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2396..2412)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2428..2450)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2855..3212
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3275..3868
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3898..4002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4003..4071,4072..4863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4221..4240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5027..5615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5516..5535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5769..5786
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
      781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
      901 atatcaccac tttgtacaag aaagctgggt cggcgcgccc accctttcac atgtgtgaga
      961 ggggcagtgt gccgttaatg gccgtgccgg gcaccgggcc gctctggtag tgctgggaca
     1021 tgtgaagtct gctgggggcg gcgggttccg gcacctcggc gccggggaga tacatgctga
     1081 tcatgtcccg gaggtccccg gcctggcagg gcgccctgga gtgggaggaa gaggtaacca
     1141 caggggggct ggagctggcc tcggacttga ccaccgaacc catggagcca agagccatgc
     1201 caggggtgcc ctgctgcgag taggacatgc tgtaggtggg cgagccgttc atgtaggtct
     1261 gcgagctggt catggagttg tactgcaggg cgctcacgtc gtagcggtgc atgggctgca
     1321 tctgcgctgc gccgtgcgca ttgaggcccg ggtgctgcgg gtagcccagc tggtcctgca
     1381 tcatgctgta gctgccgttg ctccagccgt tcatgtgcgc gtaactgtcc atgcgctggt
     1441 tcacgcccgc gcccaggccg gcgcccaccc cgaccccgct cgccatgcta ttgccgccgg
     1501 gggccagcag cccgccgggc agcgtgtact tatccttctt catgagcgtc ttggttttcc
     1561 gccggggccg gtatttataa tccgggtgct ccttcatgtg cagcgctcgc agccgcttag
     1621 cctcgtcgat gaacggccgc ttctccgtct ccgacaaaag tttccactcg gcgcccaggc
     1681 gcttgctgat ctccgagttg tgcatcttgg ggttctcctg ggccatcttg cgccgctgcc
     1741 cgcgggacca caccatgaag gcattcatgg gccgcttgac gcggtccggg ctgtttttct
     1801 ggttgccgcc ggccgccgcc gcggtggagt tgccgccgcc gccccccgaa gtttgctgcg
     1861 ggcccggcgg cttcagctcc gtctccatca tgttgtacat ggtgaagggg gcggccgcgg
     1921 agcctgcttt tttgtacaaa cttgtgattt cccgtaccac cacactggga tccttaatta
     1981 actagctaga tccggcagtc tagaggatgg tccacccccg gggtcggcag ccttcacgtg
     2041 ggcggcgtgt atccaagctg cgatgccgtc tactttgagg gcggtggggg tggtcagcag
     2101 gactgtgtaa ggtcctttcc agcgaggttc taggttctta gtctggtgtc ggcggaccca
     2161 cactgtgtcg ccgactcggt aagggtgagg taccaccggt cggtccagtt gttcttggta
     2221 ggctgccgcc agaggtctcc agacttcgtg ctggactaag tagagagcct gtaagtgagc
     2281 ttggagagag gggctgttag taactcttgt catgtcaggg tcagggaagt ttacaagggg
     2341 cgggggtgcc ccatataaga tctcatatgg ccatatgggg gcgcctagag aaggagtgag
     2401 ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg ggagagacgg
     2461 ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga cccagggagg
     2521 ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg ggtgtccatg
     2581 cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc
     2641 gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg taacccaacg
     2701 tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt tccagtgacc
     2761 taaggtcaaa cttaagggag tggtaacagt ctggccctaa ttttcagaca aatacagaaa
     2821 cacagtcaga cagagacaac acagaacgat gctgcagcag acaagacgcg cggcgcggct
     2881 tcggtcccaa accgaaagca aaaattcaga cggaggcggg aactgtttta ggttctcgtc
     2941 tcctaccaga accacatatc cctcctatta ggggggtgca ccaaagagtc caaaacgatc
     3001 gggatttttg gactcaggtc gggccacaaa aacggccccc gaagtccctg ggacgtctcc
     3061 cagggttgcg gccgggtgtt ccgaactcgt cagttccacc acgggtccgc cagatacaga
     3121 gctagttagc taactagtac cgacgcaggc gcataaaatc agtcatagac actagacaat
     3181 cggacagaca cagataagtt gctggccagc ttacctcccg gtggtgggtc ggtggtccct
     3241 gggcaggggt ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag
     3301 tcaatcactc agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc
     3361 aagagggttt attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcg
     3421 ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa
     3481 gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg
     3541 gcaccctgga aacatctgat ggttctctag aaactgctga gggctggacc gcatctgggg
     3601 accatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     3661 cccatattca gctgttccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac
     3721 agatatcctg tttggcccat attcagctgt tccatctgtt cctgaccttg atctgaactt
     3781 ttctattctc agttatgtat ttttccatgc cttgcaaaat ggcgttactt aagctagctt
     3841 gccaaaccta caggtggggt ctttcattat gcaggtggca cttttcgggg aaatgtgcgc
     3901 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3961 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4021 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4081 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4141 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4201 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4261 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4321 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4381 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4441 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4501 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4561 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4621 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4681 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4741 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4801 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4861 taactgtcag accaagttta ctcatatata ctttagattg atttgcggcc ggccgcaaaa
     4921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5161 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5761 ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
