LOCUS       sequence-483615-        9957 bp    DNA     circular SYN 18-AUG-2025
DEFINITION  Lentiviral vector driving a p53 isoform delta133p53alpha. Stable 
            expression in cultured human cells and possibly cells of other 
            species..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Ungerleider K, Beck JA, Lissa D, Joruiz S, Horikawa I, Harris CC
  TITLE     Delta133p53alpha Protects Human Astrocytes from Amyloid-beta Induced
            Senescence and Neurotoxicity.
  JOURNAL   Neuroscience. 2022 Aug 21;498:190-202. doi: 
            10.1016/j.neuroscience.2022.06.004. Epub 2022 Jun 15.
  PUBMED    35716965
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2022-08-21"; volume: "498:190-202. doi"; 
            pages: " 10.1016/j.neuroscience.2022.06.004. Epub 2022 Jun 15"
COMMENT     Sequence Label: pLOC-D133p53a
FEATURES             Location/Qualifiers
     source          1..9957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3683..3724
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3730..4302
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3897..3914)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4124..4143
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4308..4328
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4329..5021
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="ESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMK
                     STKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLH
                     VSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRT
                     FSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAFK
                     TPDADAGEE"
     misc_feature    5571..6159
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5624..5644)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6042..6053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6366..6599
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6700..6717)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6706..6930
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6976..7404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7063..7082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7273..7294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7413..7433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7418..7747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7598..7733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7660..7679
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     rep_origin      complement(7884..8472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7964..7983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8643..9434,9435..9503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9266..9285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9504..9608)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9656..9790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9693..9712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9747..9766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagatta attaagttta aacttaagct tggtaccgag
     2821 ctcggatcca ctagtccagt gtggtggaat tgatcccttc accatgtttt gccaactggc
     2881 caagacctgc cctgtgcagc tgtgggttga ttccacaccc ccgcccggca cccgcgtccg
     2941 cgccatggcc atctacaagc agtcacagca catgacggag gttgtgaggc gctgccccca
     3001 ccatgagcgc tgctcagata gcgatggtct ggcccctcct cagcatctta tccgagtgga
     3061 aggaaatttg cgtgtggagt atttggatga cagaaacact tttcgacata gtgtggtggt
     3121 gccctatgag ccgcctgagg ttggctctga ctgtaccacc atccactaca actacatgtg
     3181 taacagttcc tgcatgggcg gcatgaaccg gaggcccatc ctcaccatca tcacactgga
     3241 agactccagt ggtaatctac tgggacggaa cagctttgag gtgcgtgttt gtgcctgtcc
     3301 tgggagagac cggcgcacag aggaagagaa tctccgcaag aaaggggagc ctcaccacga
     3361 gctgccccca gggagcacta agcgagcact gcccaacaac accagctcct ctccccagcc
     3421 aaagaagaaa ccactggatg gagaatattt cacccttcag atccgtgggc gtgagcgctt
     3481 cgagatgttc cgagagctga atgaggcctt ggaactcaag gatgcccagg ctgggaagga
     3541 gccagggggg agcagggctc actccagcca cctgaagtcc aaaaagggtc agtctacctc
     3601 ccgccataaa aaactcatgt tcaagacaga agggcctgac tcagactaaa agggctcgag
     3661 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     3721 tacgcgccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     3781 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3841 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3901 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     3961 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4021 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4081 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4141 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4201 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4261 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatgccc aagaagaaga
     4321 gaaaggtgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg
     4381 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccgagcagg
     4441 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc
     4501 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga
     4561 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg
     4621 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg
     4681 gcgacttcaa ggtgatgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga
     4741 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gatctggatg
     4801 gcagcttcac ccgcaccttc agcctgcgcg acggcggcta ctacagctcc gtggtggaca
     4861 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt
     4921 tcgccttccg ccgcgtggag gaggatcaca gcaacaccga gctgggcatc gtggagtacc
     4981 agcacgcctt caagaccccg gatgcagatg ccggtgaaga aagatctcga gctcaagctt
     5041 cgaattctgc agtcgagcag ctgttgaatt ttgaccttct taagcttgcg ggagacgtcg
     5101 agtccaaccc tgggcccggt accaggaggg ccaccatgaa gaccttcaac atctctcagc
     5161 aggatctgga gctggtggag gtcgccactg agaagatcac catgctctat gaggacaaca
     5221 agcaccatgt cggggcggcc atcaggacca agactgggga gatcatctct gctgtccaca
     5281 ttgaggccta cattggcagg gtcactgtct gtgctgaagc cattgccatt gggtctgctg
     5341 tgagcaacgg gcagaaggac tttgacacca ttgtggctgt caggcacccc tactctgatg
     5401 aggtggacag atccatcagg gtggtcagcc cctgtggcat gtgcagagag ctgatctctg
     5461 actatgctcc tgactgcttt gtgctcattg agatgaatgg caagctggtc aaaaccacca
     5521 ttgaggaact catccccctc aagtacacca ggaactaata ataagttaac aatcaacctc
     5581 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5641 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5701 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5761 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5821 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5881 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5941 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6001 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6061 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6121 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     6181 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     6241 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     6301 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     6361 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6421 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6481 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6541 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6601 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6661 gagtgagagg ccttgacatt gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6721 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6781 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6841 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6901 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6961 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7021 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7081 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7141 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7201 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7261 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7321 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7381 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7501 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7561 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7621 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7681 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7741 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa
     7801 aaagcctgaa ctcaccgcga cgtcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9601 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     9661 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9721 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9781 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     9841 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     9901 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
