LOCUS       sequence-483744-        8406 bp    DNA     circular SYN 18-AUG-2025
DEFINITION  Expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Michishita E, Park JY, Burneskis JM, Barrett JC, Horikawa I
  TITLE     Evolutionarily conserved and nonconserved cellular localizations and
            functions of human SIRT proteins.
  JOURNAL   Mol Biol Cell. 2005 Oct;16(10):4623-35. doi: 
            10.1091/mbc.e05-01-0033. Epub 2005 Aug 3.
  PUBMED    16079181
REFERENCE   2  (bases 1 to 8406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.e05-01-0033"; journalName: "Mol Biol Cell"; date: 
            "2005-10"; volume: "16"; issue: "10"; pages: "4623-35"
COMMENT     Sequence Label: pcDNA-DEST47-SIRT1
FEATURES             Location/Qualifiers
     source          1..8406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    914..938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             959..3199
                     /codon_start=1
                     /product="human NAD+-dependent protein deacetylase 
                     sirtuin-1"
                     /label=SIRT1
                     /note="homolog of budding yeast Sir2"
                     /translation="MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLE
                     RSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNG
                     PGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESD
                     EEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQ
                     IVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYA
                     RLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLL
                     RNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCP
                     ADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHE
                     VPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRT
                     QKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCME
                     EKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISR
                     RLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESE
                     IEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS"
     protein_bind    complement(3219..3243)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3254..3256,3257..3259,3260..3973)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3287..3315)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3910..3930
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3995..4012)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4001..4225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4271..4699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4358..4377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4568..4589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4708..4728)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4713..5042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4893..5028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4955..4974
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5109..5903
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5163..5182)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5773..5792
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6079..6200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6116..6135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6170..6189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6249..6265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6249..6265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6262..6284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6273..6289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6297..6303,6304..6321,6322..6327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6342..6363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6480..6497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6651..7239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6731..6750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7410..8201,8202..8270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8033..8052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8271..8375)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttgat caaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat
      961 ggcggacgag gcggccctcg cccttcagcc cggcggctcc ccctcggcgg cgggggccga
     1021 cagggaggcc gcgtcgtccc ccgccgggga gccgctccgc aagaggccgc ggagagatgg
     1081 tcccggcctc gagcggagcc cgggcgagcc cggtggggcg gccccagagc gtgaggtgcc
     1141 ggcggcggcc aggggctgcc cgggtgcggc ggcggcggcg ctgtggcggg aggcggaggc
     1201 agaggcggcg gcggcaggcg gggagcaaga ggcccaggcg actgcggcgg ctggggaagg
     1261 agacaatggg ccgggcctgc agggcccatc tcgggagcca ccgctggccg acaacttgta
     1321 cgacgaagac gacgacgacg agggcgagga ggaggaagag gcggcggcgg cggcgattgg
     1381 gtaccgagat aaccttctgt tcggtgatga aattatcact aatggttttc attcctgtga
     1441 aagtgatgag gaggatagag cctcacatgc aagctctagt gactggactc caaggccacg
     1501 gataggtcca tatacttttg ttcagcaaca tcttatgatt ggcacagatc ctcgaacaat
     1561 tcttaaagat ttattgccgg aaacaatacc tccacctgag ttggatgata tgacactgtg
     1621 gcagattgtt attaatatcc tttcagaacc accaaaaagg aaaaaaagaa aagatattaa
     1681 tacaattgaa gatgctgtga aattactgca agagtgcaaa aaaattatag ttctaactgg
     1741 agctggggtg tctgtttcat gtggaatacc tgacttcagg tcaagggatg gtatttatgc
     1801 tcgccttgct gtagacttcc cagatcttcc agatcctcaa gcgatgtttg atattgaata
     1861 tttcagaaaa gatccaagac cattcttcaa gtttgcaaag gaaatatatc ctggacaatt
     1921 ccagccatct ctctgtcaca aattcatagc cttgtcagat aaggaaggaa aactacttcg
     1981 caactatacc cagaacatag acacgctgga acaggttgcg ggaatccaaa ggataattca
     2041 gtgtcatggt tcctttgcaa cagcatcttg cctgatttgt aaatacaaag ttgactgtga
     2101 agctgtacga ggagatattt ttaatcaggt agttcctcga tgtcctaggt gcccagctga
     2161 tgaaccgctt gctatcatga aaccagagat tgtgtttttt ggtgaaaatt taccagaaca
     2221 gtttcataga gccatgaagt atgacaaaga tgaagttgac ctcctcattg ttattgggtc
     2281 ttccctcaaa gtaagaccag ttgcactaat tccaagttcc ataccccatg aagtgcctca
     2341 gatattaatt aatagagaac ctttgcctca tctgcatttt gatgtagagc ttcttggaga
     2401 ctgtgatgtc ataattaatg aattgtgtca taggttaggt ggtgaatatg ccaaactttg
     2461 ctgtaaccct gtaaagcttt cagaaattac tgaaaaacct ccacgaacac aaaaagaatt
     2521 ggcttatttg tcagagttgc cacccacacc tcttcatgtt tcagaagact caagttcacc
     2581 agaaagaact tcaccaccag attcttcagt gattgtcaca cttttagacc aagcagctaa
     2641 gagtaatgat gatttagatg tgtctgaatc aaaaggttgt atggaagaaa aaccacagga
     2701 agtacaaact tctaggaatg ttgaaagtat tgctgaacag atggaaaatc cggatttgaa
     2761 gaatgttggt tctagtactg gggagaaaaa tgaaagaact tcagtggctg gaacagtgag
     2821 aaaatgctgg cctaatagag tggcaaagga gcagattagt aggcggcttg atggtaatca
     2881 gtatctgttt ttgccaccaa atcgttacat tttccatggc gctgaggtat attcagactc
     2941 tgaagatgac gtcttatcct ctagttcttg tggcagtaac agtgatagtg ggacatgcca
     3001 gagtccaagt ttagaagagc ccatggagga tgaaagtgaa attgaagaat tctacaatgg
     3061 cttagaagat gagcctgatg ttccagagag agctggagga gctggatttg ggactgatgg
     3121 agatgatcaa gaggcaatta atgaagctat atctgtgaaa caggaagtaa cagacatgaa
     3181 ctatccatca aacaaatcat ttaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     3241 ggttcgatct agaatggcta gcaaaggaga agaacttttc actggagttg tcccaattct
     3301 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     3361 tgatgctaca tacggaaagc ttacccttaa atttatttgc actactggaa aactacctgt
     3421 tccatggcca acacttgtca ctactttctc ttatggtgtt caatgctttt cccgttatcc
     3481 ggatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     3541 acgcactata tctttcaaag atgacgggaa ctacaagacg cgtgctgaag tcaagtttga
     3601 aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt gattttaaag aagatggaaa
     3661 cattctcgga cacaaactcg agtacaacta taactcacac aatgtataca tcacggcaga
     3721 caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacattg aagatggatc
     3781 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     3841 accagacaac cattacctgt cgacacaatc tgccctttcg aaagatccca acgaaaagcg
     3901 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
     3961 gctctacaaa taatgaatta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4021 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4081 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4141 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4201 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4261 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4321 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4381 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4441 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4501 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4561 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4621 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4681 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4801 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4861 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4921 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4981 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5041 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5101 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5161 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5221 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5281 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5341 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5401 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5461 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5521 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5581 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5641 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5701 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5761 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5821 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5881 gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg
     5941 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6001 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6061 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6181 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6241 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6301 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6361 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6421 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6481 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6541 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7081 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7261 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7321 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7381 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7441 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7501 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7561 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7621 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7681 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7741 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7801 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7861 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7921 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7981 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8041 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8101 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8161 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8221 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8341 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8401 gacgtc
//
