LOCUS       sequence-483753-        4173 bp    DNA     circular SYN 18-AUG-2025
DEFINITION  Donor Vector containing ZNF852 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF852_isoform1
FEATURES             Location/Qualifiers
     source          1..4173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1631..1730)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1747..1766)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1748..1766)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1771..1787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1771..1787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1889..1908)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1900..2709
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1967..1986)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2856..3444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3345..3364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3598..3615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3774..3801)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3893..3979)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4028..4050
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4042..4059
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4043..4059
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4075..4173,1)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggtcaggc cccaagacac tgttgcatat gaagacctaa gtgaagatta cacccaaaaa
       61 aaatggaaag gtttggccct cagccagaga gccctccact ggaatatgat gctagaaaat
      121 gacagatcaa tggccagcct tgggagaaac atgatggaga gttcagaatt gaccccaaaa
      181 caagaaattt ttaaaggcag tgaatcatcg aacagcacca gtggtggcct ttttggtgtt
      241 gtgcctggtg gaactgagac tggtgatgta tgtgaagaca cttttaaaga attagaaggc
      301 cagccatcga atgaagaagg gagcaggctt gagagtgatt tcctagagat catagatgag
      361 gataaaaaga agagcacgaa ggacagatat gaagaatata aagaggtaga agaacacccc
      421 cccctcagca gttcccctgt tgaacatgaa ggtgtcctga aaggccagaa atcatacaga
      481 tgtgatgagt gcgggaaagc tttttactgg agcagccacc tgattggcca cagaagaata
      541 cacactggtg agaaaccgta tgaatgtaat gaatgtggaa agactttcag acagacatcc
      601 caattgattg tccacctcag aacccatacc ggtgaaaaac catatgaatg cagtgagtgt
      661 ggtaaagcat acagacattc ttcccaccta atccaacacc agagattaca taacggagag
      721 aagccatata agtgtaatga gtgtgcaaag gcttttaacc agagttcgaa actttttgac
      781 caccaaagaa cccacaccgg agaaaagcca tatgagtgta aagaatgtgg ggcggctttt
      841 tcgaggagta aaaaccttgt gagacaccaa ttcctccata caggaaaaaa gccatataag
      901 tgtaatgaat gcggcagggc tttctgttca aacagaaacc taatagacca ccagaggacc
      961 catactggag aaaaaccgta taagtgtaat gaatgcggaa aggcattcag cagatcaaaa
     1021 tgcctaataa gacaccagag cctacacaca ggtgaaaagc catataagtg ttctgagtgt
     1081 ggaaaagcat ttaaccaaat cagccaactt gtagaacatg agaggatcca cactggagag
     1141 aagcctttta aatgttcaga gtgcggaaaa gcttttggcc tatcgaagtg cctaatcaga
     1201 caccaaagac tccacacatc tgaaaagcca tataagtgta atgaatgcgg gaagagtttt
     1261 aaccaaaatt cgtacctgat aatccaccag agaatccaca ccggtgagaa gccgtatgag
     1321 tgtaatgagt gtgggaaagt tttcagttat aattcatccc taatggtcca ccaaaggacc
     1381 cacacaggag agaaaccata taaatgtaac agctgtggga aggctttcag tgattccagc
     1441 caattgactg tacaccagag agtacatact ggtgaaaaga atttgatgaa tgtcctcagt
     1501 gttggaaagc cccttgtcag tgtgcccctt ttaataacta cttctgaatt aatgctagag
     1561 agatcgcccc aagtctggct tggccacctt ttgaaggcct ggttttctga aacagacagt
     1621 aaagacctat acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1681 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1741 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1801 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1861 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1921 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1981 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2041 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2101 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2161 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2221 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2281 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2341 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2401 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2461 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2521 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2581 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2641 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2701 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2761 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3001 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3481 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3541 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3601 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3661 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3721 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3781 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3841 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3901 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3961 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     4021 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     4081 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     4141 tttataatgc caactttgta caaaaaagtt ggc
//
