LOCUS       sequence-483778-        7289 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Expresses the codon harmonized Streptococcus pyogenes cas9 gene and 
            a NT sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Della Valle S, Orsi E, Creutzburg SCA, Jansen LFM, Pentari E, Beisel
            CL, Steel H, Nikel PI, Staals RHJ, Claassens NJ, van der Oost J, 
            Huang WE, Patinios C
  TITLE     Streamlined and efficient genome editing in Cupriavidus necator H16 
            using an optimised SIBR-Cas system
  JOURNAL   bioRxiv 2024.11.24.625072; doi: 10.1101/2024.11.24.625072
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.24.625072; doi: 10.1101/2024.11.24.625072"
COMMENT     Sequence Label: pCas9-NT
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      318..1089
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(1204..1290)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1305..1327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        join(1334..1339,1340..1357,1358..1364)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1432..5535
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1432..5535
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5536..5553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        join(5632..5637,5638..5655,5656..5662)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_RNA        5698..5773
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(6047..6066)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6058..6873
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(6944..7289,1..317))
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 tcattgacca cgtaggccga cacggtgccg gggccgttga gcttcacgac ggagatccag
       61 cgctcggcca ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc
      121 ttggaaagtg ttttctggct gaccaccacg gcgttctggt ggcccatctg cgccacgagg
      181 tgatgcagca gcattgccgc cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc
      241 gctttgcgtt ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat gccgatttct
      301 ctggactgcg tggccatgct tatctccatg cggtaggggt gccgcacggt tgcggcacca
      361 tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg
      421 cagtcaatta cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg
      481 agctgttgcg tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct
      541 atatctagtt ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg
      601 cgcggctccc cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc
      661 ttcggccttg cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc
      721 tcggggggca ggcgggcggg cttcgccctt cgactgcccc cactcgcata ggcttgggtc
      781 gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt
      841 ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca
      901 gagaaaatta aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt
      961 atgtggtcga aggctgggta gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg
     1021 cagcctgtcc atcagcttgt ccagcagggt tgtccacggg ccgagcgaag cgagccagcc
     1081 ggtggccgct cgcggccatc gtccacatat ccacgggctg gcaagggagc gcagcgaccg
     1141 cgcagggcga agcccggaga gcaagcccgt agggggggcg cgcccagctg tctagggcgg
     1201 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     1261 gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaaagcgga taacaatttc
     1321 acacaggagg ccgtttacac tttatgcttc cggctcgtat aatgtgtggg gaaaccacaa
     1381 cggtttccct ctagaaataa ttttgtttaa ctataagaag gagatataca tatggacaaa
     1441 aagtactcga ttgggctcga catcgggacc aactccgttg ggtgggctgt tatcaccgac
     1501 gagtacaaag tcccttcgaa gaaatttaaa gtcttgggga acaccgatcg tcatagcatc
     1561 aagaagaacc ttattggagc gcttctcttc gatagcgggg aaaccgctga ggctacccgg
     1621 ttgaagcgaa ccgcgcggag gaggtacacc cggcgaaaaa accggatctg ctaccttcag
     1681 gaaatcttct cgaacgaaat ggctaaggtt gacgacagct ttttccaccg tcttgaggaa
     1741 tcgttccttg ttgaggagga taaaaaacac gagcggcacc ccatcttcgg gaacattgtt
     1801 gacgaggtcg cgtaccacga aaagtacccc accatctacc acttgcgtaa gaagcttgtt
     1861 gactcgaccg acaaggctga ccttcgtctc atctaccttg cactcgctca catgatcaaa
     1921 ttccgggggc acttccttat cgaaggggac ctcaaccccg acaacagcga cgttgataag
     1981 cttttcatcc agcttgttca gacgtacaac cagctcttcg aggagaaccc catcaacgct
     2041 agcggggttg acgcgaaggc tatcctttcg gctcgtctta gcaagtcgag gcgtctcgag
     2101 aacttgatcg cgcagttgcc tggggaaaaa aagaacgggc tcttcggaaa cttgatcgcg
     2161 ctttcgcttg ggcttacgcc caacttcaag tcgaacttcg accttgctga ggacgcgaag
     2221 ctccagcttt cgaaggacac ctacgacgac gacctcgaca acctccttgc tcagatcggg
     2281 gaccagtacg cggacctttt ccttgctgcg aaaaacctct cggacgcgat cctcctttcg
     2341 gacatcctta gggttaacac cgagattacc aaagcgcctc tttcggcgtc gatgatcaag
     2401 cgttacgacg agcaccacca ggatcttacc cttctcaagg cgctcgtccg tcagcagctt
     2461 cccgagaaat acaaggagat cttcttcgac cagtcgaaga acgggtacgc tgggtacatc
     2521 gacggagggg cgtcccagga ggagttctac aagttcatca agcccatcct cgagaagatg
     2581 gacgggaccg aagagctcct tgttaagctt aaccgggagg accttttgcg taaacagcga
     2641 acgttcgata acgggtcgat ccctcaccag atccatcttg gggaattgca cgcgatcctt
     2701 aggaggcagg aggatttcta ccccttcctc aaggataacc gggaaaaaat cgagaagatc
     2761 cttaccttcc gtatccccta ctacgtcggg ccccttgctc gggggaacag ccggttcgct
     2821 tggatgaccc gaaaatcgga ggagaccatc acgccctgga acttcgagga ggtcgttgac
     2881 aagggggcgt cggcgcagtc gttcatcgag cgtatgacca acttcgacaa gaaccttccc
     2941 aacgagaagg ttcttcccaa gcacagcctt ctttacgaat acttcacggt ctacaacgag
     3001 cttaccaaag ttaagtacgt caccgagggg atgcgtaagc ccgctttcct ttcgggggag
     3061 cagaaaaagg caatcgtcga cctcttgttt aagaccaacc gtaaggttac ggtcaaacag
     3121 ctcaaggagg actactttaa gaagattgag tgcttcgaca gcgtcgagat ctcgggggtc
     3181 gaggacaggt tcaacgcgtc gctcgggacg taccacgacc ttcttaagat catcaaggac
     3241 aaggacttcc ttgacaacga ggagaacgag gacatcctcg aagacatcgt cctcaccctt
     3301 acgctcttcg aggacaggga aatgatcgaa gagaggctta agacctacgc gcatttgttc
     3361 gacgacaaag ttatgaagca gcttaagcgg cgtcggtaca ccgggtgggg gcggctttcg
     3421 cgtaagctta tcaacgggat cagggacaaa cagtcgggga agaccattct cgacttcctt
     3481 aagtcggacg ggttcgcaaa ccgtaacttc atgcagttga tccacgacga cagccttacc
     3541 ttcaaggagg atatccagaa ggctcaggtt tcggggcagg gggacagcct ccacgagcac
     3601 atcgctaacc tcgcggggtc ccccgcgatc aagaagggga tcctccagac cgttaaggtc
     3661 gtcgacgagc ttgttaaggt tatgggacga cacaaacccg agaacatcgt catcgagatg
     3721 gctcgggaga accagaccac ccagaaaggg cagaagaact cccgtgaacg gatgaagcgt
     3781 atcgaggagg ggatcaagga gctcgggagc cagatcctta aggaacaccc cgtcgagaac
     3841 acccagcttc agaacgagaa attgtacttg tactacttgc agaacgggag ggatatgtac
     3901 gttgatcagg agctcgacat caaccggctc agcgactacg acgttgacca tatcgtcccc
     3961 cagagctttc ttaaggatga ctcgattgat aacaaagttc tcacgcggtc ggacaagaac
     4021 cgggggaagt ccgacaacgt ccccagcgag gaggttgtta agaaaatgaa gaactactgg
     4081 aggcagcttc ttaacgcaaa actcatcacc cagcggaaat tcgacaacct cacgaaggcg
     4141 gagcgggggg ggcttagcga gcttgacaag gcggggttca tcaagcgtca gcttgtcgag
     4201 acccgtcaga tcaccaaaca cgttgctcag atccttgaca gccgtatgaa caccaagtac
     4261 gacgagaacg acaagcttat ccgtgaagtc aaggttatca cgctcaagtc gaagctcgtc
     4321 tcggattttc gtaaggactt tcagttttac aaggttcggg aaatcaacaa ctaccaccac
     4381 gcacacgacg cttaccttaa cgcagttgtc gggaccgcgc ttatcaaaaa gtaccccaag
     4441 cttgagtccg aattcgttta cggggactac aaggtctacg acgtccggaa gatgatcgcg
     4501 aaatcggaac aggagattgg gaaggctacg gctaagtact ttttctactc gaacatcatg
     4561 aactttttta agaccgagat cacccttgct aacggggaaa tccgtaagcg tccccttatc
     4621 gagaccaacg gagagaccgg ggagatcgtt tgggacaagg gacgtgactt cgcaaccgtt
     4681 cgtaaggttc tttccatgcc tcaggttaac atcgttaaaa agaccgaggt tcagaccggg
     4741 gggttttcca aagaatcgat cctccccaag aggaactccg ataaacttat cgcgcggaag
     4801 aaggattggg accccaagaa gtacgggggg ttcgacagcc ccacggttgc gtactcggtt
     4861 cttgttgtcg cgaaagttga gaagggaaag tccaaaaaac tcaagtccgt caaggaactc
     4921 cttggaatca ccatcatgga gaggagctcc ttcgagaaga accctatcga tttcctcgag
     4981 gcgaaggggt acaaagaggt caagaaggat ctcatcatca agcttcccaa gtacagcctt
     5041 ttcgaactcg agaacgggcg gaagcgaatg ttggcgagcg caggggagct ccagaagggg
     5101 aacgaattgg cgttgccctc caagtacgtt aacttcctct acctcgcgag ccactacgag
     5161 aaacttaaag ggagccccga ggacaacgag cagaagcagc ttttcgttga acagcacaaa
     5221 cactacctcg acgaaatcat cgaacagatc agcgagttct cgaaacgggt catcctcgct
     5281 gacgcaaacc tcgacaaggt ccttagcgct tacaacaagc acagggataa gcccattcgg
     5341 gagcaggctg agaacatcat ccacctcttc acgcttacga accttggggc gcctgcggcg
     5401 ttcaagtact tcgacaccac catcgaccgg aagcgttaca cgtcgaccaa ggaggtcctc
     5461 gacgcaaccc ttatccacca gtccatcacc gggctttacg agacccgtat cgaccttagc
     5521 cagcttgggg gggatcacca tcaccatcac cattagcggc cgcataatga tgtgttatca
     5581 ttgatgcgag gtcgcctata cctccccgct tcggcggggt tttttcccgg gtttacactt
     5641 tatgcttccg gctcgtataa tggatggcag gtgccccaac cctacccacc tgctagagtt
     5701 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     5761 accgagtcgg tgctttgatc cggtggatga ccttttgaat gacctttaat agattatatt
     5821 actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt ttcacaaaac
     5881 ggtttacaag cataaagctt gctcaatcaa tcaccgtcga cctcgtctcc ccgcttcggc
     5941 ggggtttttt cctaggttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     6001 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     6061 agccatattc agcgtgaaac gagctgtagc cgtccgcgtc tgaacagcaa catggatgcg
     6121 gatctgtatg gctataaatg ggcgcgtgat aacgtgggtc agagcggcgc gaccatttat
     6181 cgtctgtatg gcaaaccgga tgcgccggaa ctgtttctga aacatggcaa aggcagcgtg
     6241 gcgaacgatg tgaccgatga aatggtgcgt ctgaactggc tgaccgaatt tatgccgctg
     6301 ccgaccatta aacattttat tcgcaccccg gatgatgcgt ggctgctgac caccgcgatt
     6361 ccgggcaaaa ccgcgtttca ggtgctggaa gaatatccgg atagcggcga aaacattgtg
     6421 gatgcgctgg ccgtgtttct gcgtcgtctg catagcattc cggtgtgcaa ctgcccgttt
     6481 aacagcgatc gtgtgtttcg tctggcccag gcgcagagcc gtatgaacaa cggcctggtg
     6541 gatgcgagcg attttgatga tgaacgtaac ggctggccgg tggaacaggt gtggaaagaa
     6601 atgcataaac tgctgccgtt tagcccggat agcgtggtga cccacggcga ttttagcctg
     6661 gataacctga ttttcgatga aggcaaactg attggctgca ttgatgtggg ccgtgtgggc
     6721 attgcggatc gttatcagga tctggccatt ctgtggaact gcctgggcga atttagcccg
     6781 agcctgcaaa aacgtctgtt tcagaaatat ggcattgata atccggatat gaacaaactg
     6841 caatttcatc tgatgctgga tgaatttttc taataattaa ttggaccgcg gtccgagaga
     6901 cgcatggcag tatctcagac tgtgtatcgg cacgtctcac tacttaccgg cgcggcagcg
     6961 ttacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca
     7021 tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt
     7081 ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta
     7141 gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg
     7201 attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact
     7261 ggtcgcgggg ctggccccac gccacgcgg
//
