LOCUS       sequence-483810-       12288 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Express H3-NLS-EGFP in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12288)
  TITLE     A Toolbox of TEF1-Intron-Based Strong Promoter Reporter Systems for 
            Filamentous Fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGY245
FEATURES             Location/Qualifiers
     source          1..12288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1315..1317,1318..2031)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1315..1317,1318..2028)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1357..1378)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1618..1637)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1965..1986
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2854..3879
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3951..3984)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4454..4470)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4569..4586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4740..5328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4820..4839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5450..6241,6242..6310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6073..6092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6311..6402)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6444..6463
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6469..6488
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    6695..6728
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7041..12288
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggcccgca ctaagcagac tgcccgtaag tccactggtg gcaaggctcc ccgtaagcag
     1201 ctcgcctcca aggctgcccg taaggccgct ccctccaccg gaggtgtcaa gaagcctcac
     1261 cgttacaagc ccggtaccgt cgctctgcgt gaaatccgtc gttaccagaa gagcgtgagc
     1321 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1381 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1441 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1501 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1561 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1621 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1681 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1741 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1801 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1861 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1921 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1981 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggacatt
     2041 cgatttatgc cgttatgact tccttaaaaa agcctttacg aatgaaagaa atggaattag
     2101 acttgttatg tagttgattc tacaatggat tatgattcct gaacttcaaa tccgctgttc
     2161 attattaatc tcagctcttc ccgtaaagcc aatgttgaaa ctattcgtaa atgtacctcg
     2221 ttttgcgtgt accttgctta tcacgtgata ttacatgacc tggacagagt tctgcgcgaa
     2281 agtcataacg taaatcccgg gcggtaggtg cgtcccgggc ggaaggtagt tttctcgtcc
     2341 accccaacgc gtttatcaac ctcaactttc aacaaccatc atgccaccaa aagcgcgtaa
     2401 aacaaagcga gatttgattg agcaagaggg caggatccaa tgcgcgattc aagacattaa
     2461 aaatggaaaa tttcaaaaaa ttgcgcccgc agcgcgtgca tacaaaattc atcccaatac
     2521 aagtgtaatg ctagtggagg tcaacacatc aatgcctatt ttggtttagt cgtccaggcg
     2581 gtgagcacaa aatttgtgtc gtttgacaag atggttcatt taggcaactg gtcagatcag
     2641 ccccacttgt agcagtagcg gcggcgctcg aagtgtgact cttattagca gacaggaacg
     2701 aggacattat tatcatctgc tgcttggtgc acgataactt ggtgcgtttg tcaagcaagg
     2761 taagtggacg acccggtcat accttcttaa gttcgccctt cctcccttta tttcagattc
     2821 aatctgactt acctattcta cccaagcatc caaatgaaaa agcctgaact caccgcgacg
     2881 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     2941 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     3001 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     3061 gccgcgctcc cgattccgga agtgcttgac attggggagt tcagcgagag cctgacctat
     3121 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     3181 gctgttctcc agccggtcgc ggaggccatg gatgcgattg ctgcggccga tcttagccag
     3241 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     3301 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     3361 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     3421 gaagtccggc acctcgtgca tgcggatttc ggctccaaca atgtcctgac ggacaatggc
     3481 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     3541 gccaacatcc tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     3601 gagcggaggc atccggagct tgcaggatcg ccgcgcctcc gggcgtatat gctccgcatt
     3661 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     3721 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     3781 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     3841 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgatcccc
     3901 actaccgcat taagacctca gcgcggccgc aaatttaaat aaaatgaagt gaagttccta
     3961 tactttctag agaataggaa cttctatagt gagtcgaata agggcgacac aaaatttatt
     4021 ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt gtattatcgt
     4081 tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc gagatttatt
     4141 ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga
     4201 tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc
     4261 gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc
     4321 gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg
     4381 aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg
     4441 gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa
     4501 aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct
     4561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct
     5161 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5341 atcttttcta cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga
     5401 ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgct taccaatgct
     5461 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga
     5581 tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg
     5641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5761 tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5941 cagcgctaca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6061 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6301 gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa
     6421 ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac
     6481 accccttgtt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     6541 gaagtgaaac gccgtagcgc cgatggtagt gtggggactc cccatgcgag agtagggaac
     6601 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gcccgggcta
     6661 attatggggt gtcgccctta ttcgactcta tagtgaagtt cctattctct agaaagtata
     6721 ggaacttctg aagtggggat ttaaatgcgg ccgcgctgag ggtttaatcg acgaagcagc
     6781 tgacggccag tgccaagctt aacgcgtacc gggcccagta tatgttccgc agatgactgg
     6841 agctctgcca tacgtgccct ctcaagcacc atttgttcca tctacagaga ctagtcacca
     6901 actagtctat caagactcac agggtacatt gctgagacca actgaccaga ggcagggtag
     6961 cggattgacg gctccatctc cttcacttac aaggtctatt gaaagccctt tagcatcacc
     7021 aagcggagaa tagattgtta agcttatttt ttgtatactg ttttgtgata gcacgaagtt
     7081 tttccacggt atcttgttaa aaatatatat ttgtggcggg cttacctaca tcaaattaat
     7141 aagagactaa ttataaacta aacacacaag caagctactt tagggtaaaa gtttataaat
     7201 gcttttgacg tataaacgtt gcttgtattt attattacaa ttaaaggtgg atagaaaacc
     7261 tagagactag ttagaaacta atctcaggtt tgcgttaaac taaatcagag cccgagaggt
     7321 taacagaacc tagaagggga ctagatatcc gggtagggaa acaaaaaaaa aaacaagaca
     7381 gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat aaaagacaat
     7441 gataccacag tctagttgac aactagatag attctagatt gaggccaaag tctctgagat
     7501 ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct gaagctagaa
     7561 taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga ttgcaaggtg
     7621 ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt attaacctac
     7681 catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc tcattttcat
     7741 gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac cacaaaggag
     7801 aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt ctgcaactag
     7861 ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat tgttggagga
     7921 actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca ataactagtt
     7981 ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg
     8041 atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct gagactagtt
     8101 agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt gcgacaggtc
     8161 ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc ctcacggcgg
     8221 atctgcataa agagtggcta gaggttataa attagcactg atcctaggta cggggctgaa
     8281 tgtaacttgc ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag
     8341 tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag ttagcaacta
     8401 gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat ttccagcttc
     8461 accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat gggagaagaa
     8521 actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct gctatatatt
     8581 agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct tcttggtagg
     8641 agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta cttgactagt
     8701 cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat cagttccttc
     8761 atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt atccttcaag
     8821 tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa taactagtca
     8881 gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact cggcagtaga
     8941 cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc agtaactagg
     9001 cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc tccccgtcct
     9061 taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta tcgcgaatgc
     9121 tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga ttggaggagg
     9181 aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct atctctaagc
     9241 agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg actagttatg
     9301 tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa
     9361 aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg ctaaccataa
     9421 acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc gaccaccgag
     9481 gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac aaccccacct
     9541 tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca gaaaggcaag
     9601 gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg ttaactaggt
     9661 caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc caaacataca
     9721 cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga gcccagccat
     9781 ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc aatggaaacg
     9841 gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc taagtcgacc
     9901 atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt acctcttgac
     9961 cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta tatccaaact
    10021 ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga tacttaacct
    10081 tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc actctcacct
    10141 tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc aacgattagt
    10201 atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca ctatctcgag
    10261 gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg acccgactag
    10321 ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga tatccatcca
    10381 tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct actgctatac
    10441 tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga aacatattgg
    10501 tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac aatggacctc
    10561 ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac ttggtgacta
    10621 gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg ttctcccaac
    10681 cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc taactagtgt
    10741 attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt gtcgagaatt
    10801 cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac tttgcagcaa
    10861 cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac agccattcaa
    10921 tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc cgtgcatacc
    10981 cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa aggaagggca
    11041 agttacattc agccccgtac ctaggatcag tgctaattta taacctctag ccactcttta
    11101 tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa ggaaccatac
    11161 cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta gtttgggact
    11221 agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt ttggcgcttt
    11281 ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc agactcagac
    11341 aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc ttgaggccct
    11401 ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt cactaactag
    11461 actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag aaagtggtaa
    11521 gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt aaacaaggaa
    11581 aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca gggtgatggc
    11641 agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc aataactagt
    11701 ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg ataatagtag
    11761 taagttattc tagcttcaga gttataggag actagatact aactagtatt agttgcaact
    11821 aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc aactagactg
    11881 tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag tctccctaat
    11941 atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc cccttctagg
    12001 ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta gtttctaact
    12061 agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa cgtttatacg
    12121 tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta gtttataatt
    12181 agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt aacaagatac
    12241 cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagct
//
