LOCUS       sequence-483814-        8850 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Sleeping Beauty XPRESSO vector for eGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8850)
  TITLE     XPRESSO: Rapid Genetic Engineering of Human Pluripotent Stem Cells 
            for Durable Overexpression Using a Modular Anti-silencing Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSB-XPRESSO-EGFP
FEATURES             Location/Qualifiers
     source          1..8850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    324..548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     537..554
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    587..1175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             693..704
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1102..1122
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1206..1919,1920..1922,1923..1925))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1209..1919,1920..1922,1923..1925))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1251..1272)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1600..1619
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1859..1880
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1922..1931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1996..2015)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2023..3039
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2077..2099)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3040..3316)
                     /label=chicken beta-actin promoter
     enhancer        3318..3697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             4868..5569
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5001..5019)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5269..5288
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5463..5486
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5579..5635
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5636..6235
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5636..5655)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6132..6152
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6335..6354)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6400..6455
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6454..6473)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6847..6864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7018..7606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7098..7117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7777..8568,8569..8637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8400..8419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8638..8742)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca
      301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
      361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
      421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
      481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
      541 aggcacagtc gaggctgatc agcgagctca ggtcgacggt atcgatgcgg ggaggcggcc
      601 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
      661 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
      721 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
      781 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
      841 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
      901 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
      961 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     1021 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     1081 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     1141 aatctttcac aaattttgta atccagaggt tgattatcga aagcttggcc tgacaggccg
     1201 tctcattact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     1261 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     1321 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     1381 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     1441 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     1501 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     1561 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     1621 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     1681 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     1741 acgagggtgg gccagggcac gggcagcttg ccggtggtgc aaatgaactt cagggtcagc
     1801 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     1861 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     1921 accatggtgg cggcctcaga ggccaacagc ggccgcgata tcctcgagga attctttgcc
     1981 aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa
     2041 ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc
     2101 cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag
     2161 agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct
     2221 gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga
     2281 tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc acgattacca
     2341 taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc
     2401 gcgcccctcc cccgagcccy ccccggcccg aggcggcccc gccccgcccg gcacccccmc
     2461 ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac ggagccccgc
     2521 acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg gttgcagccc
     2581 gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca cgcagccttt
     2641 gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg
     2701 cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag
     2761 cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc ccgagccgct
     2821 ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga
     2881 aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
     2941 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc
     3001 ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt
     3061 tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct
     3121 ctgattggct gccgccscac ctctccscct csccccsccc cscccctcsc cccccccccc
     3181 cccccctggg cgcgcccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa
     3241 ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac
     3301 gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     3361 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     3421 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     3481 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     3541 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     3601 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     3661 attgattact attaataact agtcaataat caatgtcgac tcgtattccc gggctgccaa
     3721 gcagaaaagc caccgctgag gagactccgg tcactgtcct cgccccgcct cccccttccc
     3781 tccccttggg gaccaccggg cgccacgccg cgaacggtaa gtgccgcggt cgtcggcgcc
     3841 tccgccctcc ccctagggcc ccaattccca gcgggcgcgg cgccggcccc tccccccgcc
     3901 gcgcgcgcgc ccgctgcccc gcccttcgtg gccgcccggc gtgggcggtg ccacccctcc
     3961 ccccggcggc cccgcgcgca gctcccggct ccctccccct tcggatgtgg cttgagctgt
     4021 aggcgcggag ggccggagac gctgcagacc cgcgacccgg agcagctcgg aggcggtgaa
     4081 gtcggtggct ttccttctct ctagctctcg ctcgctggtg gtgcttcaga tgccacacgc
     4141 gtagcttata ataccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg
     4201 cgatgtgcgc tctgcccact gacgggcacc ggagcctcta ggcgagaccc tgtctcacaa
     4261 aataaagtaa gcccggactg agtgcggaaa ggcgggcctg gcgggtctgg tctccccatg
     4321 cgggccacca gaggccctgc agccttcagt cgcttgaagg ggtaatggcg cttccactca
     4381 caaacatggc ggacagagcg tgtgaacgag atgaacagcc cctcaaaaat atggccgccg
     4441 aggctggacg gccgtgcccc agcagcaccg cctccgcgcc ccacgtgatc tctcgccggg
     4501 cacagcgctg accgcggagg tccaaccgga agaatgtccg gattggacat tcggaagagg
     4561 gcccgccttc cctggggaat ctctgcgcac gcgcagaacg cttcgaccaa tgaaaacaca
     4621 ggaagccgtc cgcgcaaccg cgttgcgtca cttctgccgc ccctgtttca aggtatatag
     4681 ccgtagacgg aacttcgcct ttctctcggc cttagcgcca tttttttggg tgagtgtttt
     4741 ttggttcctg cgttgggatt ccgtgtacaa tccatagaca tctgacctcg gcacttagca
     4801 tcatcacagc aaactaactg tagcctttct ctctttccct gtagaaacct ctgcacctga
     4861 ggccaccatg ttgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
     4921 catggagggc tccatgaacg gccatgagtt cgagatcgag ggcgagggcg agggccgccc
     4981 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     5041 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     5101 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5161 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     5221 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     5281 gaagaagaca atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     5341 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     5401 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     5461 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     5521 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagg gcagtggagc
     5581 tactaacttc agcctgctga agcaggctgg tgacgtcgag gagaatcctg gccccatgac
     5641 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     5701 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     5761 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5821 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     5881 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     5941 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6001 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6061 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6121 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6181 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgattcga
     6241 aggcctgtcg tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     6301 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     6361 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     6421 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     6481 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct
     6541 ggctgccatg aactagctac tcgggacccc ttaccgaaac atcgccgcat tctgcagagg
     6601 agtcgagtgt atgtaaactt ctgacccact gggaatgtga tgaaagaaat aaaagctgaa
     6661 atgaatcatt ctctctacta ttattctgat atttcacatt cttaaaataa agtggtgatc
     6721 ctaactgacc taagacaggg aatttttact aggattaaat gtcaggaatt gtgaaaaagt
     6781 gagtttaaat gtatttggct aaggtgtatg taaacttccg acttcaactg tatagggatc
     6841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8761 gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     8821 aattgtaagc gttaatattt tgttaaaatt
//
